Mediator Requirement Downstream of Chromatin Remodeling during Transcriptional Activation of CHA1 in Yeast

He, Qiye; Battistella, Luisa; Morse, Randall H.

doi:10.1074/jbc.m708266200

Cited by 29 publications

(54 citation statements)

References 89 publications

(128 reference statements)

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“…This notion was further supported by recent findings that Mediator can also stimulate basal transcription in vitro (21). Consistent with these findings, Mediator is recruited to the promoters of many inducible genes in both yeast and higher eukaryotes (18,29,40,41). However, it remained possible that Mediator does not function directly at all genes, and that transcriptional defects caused by loss of Mediator could be the result of indirect effects at many genes.…”

Section: Discussionsupporting

confidence: 73%

“…Previously we reported recruitment of the tail module of Mediator to the CHA1 promoter, even in the absence of head and middle module recruitment, in srb4 ts yeast (29). Here we find similar independent recruitment of the tail module to 3 RP genes and to CDC19 in srb4 ts yeast, despite loss of head and middle module recruitment.…”

Section: Discussionsupporting

confidence: 65%

“…Previously we showed that in srb4 ts mutant yeast at nonpermissive temperature, recruitment of Srb5 and Rgr1 from the head and middle modules of Mediator to the induced CHA1 promoter was reduced, while recruitment of Gal11 from the tail module was unimpaired (29). To examine Gal11 association with constitutively transcribed gene promoters, we again used WT and srb4 ts yeast strains expressing a Myc 13 -tagged Gal11 subunit and performed ChIP from yeast after a 45-min shift to 37°C.…”

Section: Independent Recruitment Of the Tail Module Of Mediatormentioning

confidence: 99%

“…We reasoned that by comparing Mediator association at promoter regions in WT and srb4 ts yeast, relative to association with a control nontranscribed region, even low levels of functionally significant association could be detected. To test this hypothesis, we generated isogenic WT and srb4 ts strains from yeast expressing Myc 13 -tagged Med18/Srb5 or Med15/Gal11 or TAP-tagged Med14/Rgr1 (29). The WT and mutant strains were grown in complete synthetic medium (CSM) at 25°C, rapidly shifted to 37°C, and incubated at 37°C for 45 min to inactivate the Srb4 subunit in the srb4 ts mutant before carrying out ChIP.…”

Section: Mediator Associates Specifically With Several Constitutivelymentioning

confidence: 99%

“…The WT and mutant strains were grown in complete synthetic medium (CSM) at 25°C, rapidly shifted to 37°C, and incubated at 37°C for 45 min to inactivate the Srb4 subunit in the srb4 ts mutant before carrying out ChIP. Serine was added to the medium to induce the CHA1 gene to provide a positive control for Mediator recruitment (29). Association of Mediator subunits with both core promoter (Ϫ100 bp to ϩ100 bp) and upstream activator sequence (UAS) regions (spanning Rap1 binding sites from 300 to 650 bp upstream of the starting ATG for the RP genes and CDC19, and between 150 bp and 250 bp upstream of the starting ATG for CHA1) was quantified by real time PCR and was normalized to input DNA and to a nontranscribed region of ChrV.…”

Section: Mediator Associates Specifically With Several Constitutivelymentioning

confidence: 99%

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Mediator complex association with constitutively transcribed genes in yeast

Ansari

Morse

2009

Proc. Natl. Acad. Sci. U.S.A.

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Mediator is a large, multisubunit complex that is essential for transcription of mRNA by RNA Pol II in eukaryotes and is believed to bridge transcriptional activators and the general transcription machinery. However, several recent studies suggest that the requirement for Mediator during transcriptional activation is not universal, but rather activator dependent, and may be indirect for some genes. Here we have investigated Mediator association with several constitutively transcribed genes in yeast by comparing a yeast strain that harbors a temperature-sensitive mutation in an essential Mediator subunit, Srb4, with its wild-type (WT) counterpart. We find modest association of Mediator with constitutively active genes and show that this association is strongly decreased in srb4 ts yeast, whereas association with a nontranscribed region or repressed gene promoters is lower and unaffected in the mutant yeast. The tail module of Mediator remains associated with ribosomal protein (RP) gene promoters in srb4 ts yeast, while subunits from the head and middle modules are lost. Tail module association at Rap1-dependent gene promoters is lost in rap1 ts yeast, indicating that Rap1 is required for Mediator recruitment at these gene promoters and that its recruitment occurs via the tail module. Pol II association is also rapidly and severely affected in srb4 ts yeast, indicating that Mediator is directly required for pol II association at constitutively transcribed genes. Our results are consistent with Mediator functioning as a general transcription factor in yeast. Rap1 ͉ transcription

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Section: Discussionsupporting

confidence: 73%

Section: Discussionsupporting

confidence: 65%

Section: Independent Recruitment Of the Tail Module Of Mediatormentioning

confidence: 99%

Section: Mediator Associates Specifically With Several Constitutivelymentioning

confidence: 99%

Section: Mediator Associates Specifically With Several Constitutivelymentioning

confidence: 99%

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Mediator complex association with constitutively transcribed genes in yeast

Ansari

Morse

2009

Proc. Natl. Acad. Sci. U.S.A.

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Luminescent Organic 1D Nanomaterials Based on Bis(β‐diketone)carbazole Derivatives

Liu

et al. 2011

Chemistry A European J

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A series of new triphenylamine-functionalized bis(β-diketone)s bridged by a carbazole (CnBDKC, n=1, 4, 8, 16) with twisted intramolecular charge-transfer emission in polar solvents has been synthesized. The length of the carbon chains has a significant effect on the self-assembling properties of the compounds. Well-defined 1D nanowires were easily generated from C1BDKC with a methyl group by a reprecipitation approach directed by π-stacking interaction, and the molecules packed into J-aggregates in the nanowires. In addition, 1D nanofibers based on C16BDKC bearing a long hexadecyl chain were prepared through the organogelation process, and H-aggregates were formed driven by the synergistic effect of π-stacking interaction and van der Waals force in the gel phase. C4BDKC and C8BDKC containing butyl and octyl side chains, respectively, cannot arrange into dispersed nanostructures, probably because π-π interaction between conjugated moieties might be disturbed by the interaction between the side chains, which is, however, not strong enough to dominate the self-assembling process. Notably, the nanowires based on C1BDKC and the gel nanofibers from C16BDKC can emit strong green light under irradiation, which suggests that these 1D nanomaterials may have potential applications in emitting materials as well as photonic devices.

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Mediator Requirement Downstream of Chromatin Remodeling during Transcriptional Activation of CHA1 in Yeast

Cited by 29 publications

References 89 publications

Mediator complex association with constitutively transcribed genes in yeast

Mediator complex association with constitutively transcribed genes in yeast

Luminescent Organic 1D Nanomaterials Based on Bis(β‐diketone)carbazole Derivatives

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