Mediator, known as a coactivator, can act in transcription initiation in an activator-independent manner in vivo

Jean-Jacques, Hélène; Poh, Siew Lay; Kuras, Laurent

doi:10.1016/j.bbagrm.2018.07.001

Cited by 5 publications

(2 citation statements)

References 59 publications

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“…Mediator (MED) is a coactivator that connects transcriptional regulators and RNA polymerase (Pol) II and initiates gene transcription. [ 31 32 ] MED is a multiprotein complex consisting of more than 30 subunits and four modules: kinase, head, middle, and tail. [ 33 ] Mediator 17 (MED17), a subunit of the head module of MED, interacts with other subunits and is a key scaffold component.…”

Section: Discussionmentioning

confidence: 99%

Explore the role of long noncoding RNAs and mRNAs in intracranial atherosclerotic stenosis: From the perspective of neutrophils

Wang,

Han

et al. 2023

Brain Circulation

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CONTEXT: Circulating neutrophils and long noncoding RNAs (lncRNAs) play various roles in intracranial atherosclerotic stenosis (ICAS). OBJECTIVE: Our study aimed to detect differentially expressed (DE) lncRNAs and mRNAs in circulating neutrophils and explore the pathogenesis of atherosclerosis from the perspective of neutrophils. METHODS: Nineteen patients with ICAS and 15 healthy controls were enrolled. The peripheral blood of the participants was collected, and neutrophils were separated. The expression profiles of lncRNAs and mRNAs in neutrophils from five patients and five healthy controls were obtained, and DE lncRNAs and mRNAs were selected. Six lncRNAs were selected and validated using quantitative reverse transcription–polymerase chain reaction (qRT-PCR), and ceRNA and lncRNA-RNA binding protein (RBP)-mRNA networks were constructed. Correlation analysis between lncRNAs and mRNAs was performed. Functional enrichment annotations were also performed. RESULTS: Volcano plots and heat maps displayed the expression profiles and DE lncRNAs and mRNAs, respectively. The qRT-PCR results revealed that the four lncRNAs showed a tendency consistent with the expression profile, with statistical significance. The ceRNA network revealed three pairs of regulatory networks: lncRNA RP3-406A7.3-NAGLU, lncRNA HOTAIRM1-MVK/IL-25/GBF1/CNOT4/ANKK1/PLEKHG6, and lncRNA RP11-701H16.4-ZNF416. The lncRNA-RBP-mRNA network showed five pairs of regulatory networks: lncRNA RP11-701H16.4-TEK, lncRNA RP11-701H16.4-MED17, lncRNA SNHG19-NADH-ubiquinone oxidoreductase core subunit V1, lncRNA RP3-406A7.3-Angel1, and lncRNA HOTAIRM1-CARD16. CONCLUSIONS: Our study identified and verified four lncRNAs in neutrophils derived from peripheral blood, which may explain the transcriptional alteration of neutrophils during the pathophysiological process of ICAS. Our results provide insights for research related to the pathogenic mechanisms and drug design of ICAS.

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Section: Discussionmentioning

confidence: 99%

Explore the role of long noncoding RNAs and mRNAs in intracranial atherosclerotic stenosis: From the perspective of neutrophils

Wang,

Han

et al. 2023

Brain Circulation

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“…Indeed, a recent proteomic analysis of Mediator interactions reported a modest interaction between Mediator and Tbf1 (55). Mediator could also be recruited to sn/snoRNA genes independently of activators via interactions with the PIC (34,(56)(57)(58).…”

Section: Discussionmentioning

confidence: 99%

Mediator Is Essential for Small Nuclear and Nucleolar RNA Transcription in Yeast

Tourigny

Saleh

Schumacher

et al. 2018

Molecular and Cellular Biology

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Eukaryotic RNA polymerase II (RNAPII) transcribes mRNA genes and non-protein-coding RNA (ncRNA) genes, including those encoding small nuclear and nucleolar RNAs (sn/snoRNAs). In metazoans, RNAPII transcription of sn/snoRNAs is facilitated by a number of specialized complexes, but no such complexes have been discovered in yeast. It has been proposed that yeast sn/snoRNA and mRNA expression relies on a set of common factors, but the extent to which regulators of mRNA genes function at yeast sn/snoRNA genes is unclear. Here, we investigated a potential role for the Mediator complex, essential for mRNA gene transcription, in sn/ snoRNA gene transcription. We found that Mediator maps to sn/snoRNA gene regulatory regions and that rapid depletion of the essential structural subunit Med14 strongly reduces RNAPII and TFIIB occupancy as well as nascent transcription of sn/ snoRNA genes. Deletion of Med3 and Med15, subunits of the activator-interacting Mediator tail module, does not affect Mediator recruitment to or RNAPII and TFIIB occupancy of sn/snoRNA genes. Our analyses suggest that Mediator promotes PIC formation and transcription at sn/snoRNA genes, expanding the role of this critical regulator beyond its known functions in mRNA gene transcription and demonstrating further mechanistic similarity between the transcription of mRNA and sn/snoRNA genes. E ukaryotic RNA polymerase II (RNAPII), responsible for the transcription of mRNA genes, also transcribes several classes of non-protein-coding RNA (ncRNA) genes. Two prominent classes of RNAPII-transcribed eukaryotic ncRNA genes are small nuclear RNAs (snRNAs) and small nucleolar RNAs (snoRNAs). snRNAs are involved in pre-mRNA splicing (1), while snoRNAs primarily function in rRNA maturation via guiding methylation and pseudouridylation of specific rRNA bases (2).Most sn/snoRNA transcription in eukaryotes is carried out by RNAPII, with a few notable RNAPIII-transcribed exceptions, including the U6 snRNA gene (3). While transcription of both mRNA and the majority of sn/snoRNA genes is carried out by RNAPII, notable differences between their regulation have been described, particularly in metazoans. In addition to the documented dependence of a few sn/snoRNAs on several general transcription factors (GTFs) that are components of the preinitiation complex and generally essential for RNAPII transcription (TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH) (4-6), metazoan sn/snoRNA gene transcription relies on a number of specialized factors. One such factor is the small nuclear RNA activating complex (SNAPc), which contains the TATA-binding protein (TBP) as well as five specific subunits and is essential for

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Crosstalk of promoter and terminator during RNA polymerase II transcription cycle

Al-Husini

Medler

Ansari

2020

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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Mediator, known as a coactivator, can act in transcription initiation in an activator-independent manner in vivo

Cited by 5 publications

References 59 publications

Explore the role of long noncoding RNAs and mRNAs in intracranial atherosclerotic stenosis: From the perspective of neutrophils

Explore the role of long noncoding RNAs and mRNAs in intracranial atherosclerotic stenosis: From the perspective of neutrophils

Mediator Is Essential for Small Nuclear and Nucleolar RNA Transcription in Yeast

Crosstalk of promoter and terminator during RNA polymerase II transcription cycle

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