Mediation of attachment of Burkholderia pseudomallei to human pharyngeal epithelial cells by the asialoganglioside GM1-GM2 receptor complex.

Gori, Ahmad Hamid; Ahmed, Kamruddin; Martinez, Glenda; Masaki, Hironori; Watanabe, Kiwao; Nagatake, Tsuyoshi

doi:10.4269/ajtmh.1999.61.473

Cited by 18 publications

(14 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Galactose, glucose and N-acetylgalactosamine (1 mg ml À1 ) have been demonstrated previously to inhibit attachment, along with asialo-GM1 (12 . 5 ìg ml À1 ) confirming the binding action of this glycolipid (Gori et al, 1999). In contrast to this study, mannose was not shown to inhibit attachment, however the concentration used was 25-fold less.…”

Section: Oligosaccharide Specificity In Relation To Bacterial Adhesinscontrasting

confidence: 52%

“…In contrast to this study, mannose was not shown to inhibit attachment, however the concentration used was 25-fold less. It is well established that asialo-GM1 and asialo-GM2 are host cell receptors for Burkholderia pseudomallei (Kanai et al, 1997;Gori et al, 1999), however, the adhesins they bind have not been characterized. Possible adhesins identified include the capsule, LPS and flagellum (Atkins & Oyston, 2003).…”

Section: Oligosaccharide Specificity In Relation To Bacterial Adhesinsmentioning

confidence: 99%

See 1 more Smart Citation

Common oligosaccharide moieties inhibit the adherence of typical and atypical respiratory pathogens

Thomas

Brooks

2004

Journal of Medical Microbiology

View full text Add to dashboard Cite

Intervention in bacterial adhesion to host cells is a novel method of overcoming current problems associated with antibiotic resistance. Antibiotic-resistant strains of bacteria that cause respiratory tract infections are a problem in hospitals and could be used in bioterrorist attacks. A range of bacterial species was demonstrated to attach to an alveolar epithelial (A549) cell line. In all cases, cell surface oligosaccharides were important in attachment, demonstrated by reduced adhesion when A549 cells were pre-treated with tunicamycin. Bacillus anthracis and Yersinia pestis displayed a restricted tropism for oligosaccharides compared to the environmental, opportunistic pathogens, Pseudomonas aeruginosa, Burkholderia cenocepacia, Burkholderia pseudomallei and Legionella pneumophila. The compound with the greatest anti-adhesion activity was p-nitrophenol. Other generic attachment inhibitors included the polymeric saccharides (dextran and heparin), GalNAcâ1-4Gal, GalNAcâ1-3Gal, Galâ1-4GlcNAc and Galâ1-3GlcNAc. Burkholderia pseudomallei attachment was particularly susceptible to oligosaccharide inhibition. Combinations of such compounds may serve as a novel generic therapeutics for respiratory tract infections. INTRODUCTIONThe development of antibiotic resistance amongst pathogenic bacteria is a frequent occurrence and of great concern for public health. This problem is associated with many bacteria that cause community-and hospital-acquired pneumonia such as Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonas aeruginosa and Burkholderia cenocepacia (Lister, 2000;Lynch, 2001;Spencer, 1995;Tan, 2003). Problems with antibiotic resistance can also extend to those bacteria that could be used in bioterrorism. It is likely that such bacterial threat agents would be delivered by the aerosol route resulting in respiratory presentation of the disease (Cieslak & Eitzen, 2000;Leggiadro, 2000). Given the ease of natural acquisition of antibiotic resistance genes and genetic manipulation, it is prudent to research therapeutic alternatives to antibiotics.Attachment to cell surfaces is one of the key steps in bacterial pathogenesis, often involving oligosaccharides located on the host cell surface and bacterial adhesins (Karlsson et al., 1992;Ofek & Sharon, 1990). The terminal di-or trisaccharide units of these oligosaccharides may be used to inhibit these interactions, preventing attachment and hence disease (Zopf & Roth, 1996). The theory has been proven in vitro with a range of bacterial species. For example, lacto-N-neotetraose and asialoganglioside-GM1 (asialo-GM1) inhibit attachment of S. pneumoniae to alveolar epithelial cells (Tong et al., 1999). Similarly 3-sialyllactose inhibits Helicobacter pylori attachment to gastric epithelial cells . Asialoganglioside-GM1 and -GM2 are used as receptors by a number of respiratory pathogens, which specifically bind their terminal GalNAcâ1-3Gal and GalNAcâ1-4Gal moieties (Krivan et al., 1988a(Krivan et al., , b, 1991. The principle has also been proven to be valid in vivo...

show abstract

Section: Oligosaccharide Specificity In Relation To Bacterial Adhesinscontrasting

confidence: 52%

Section: Oligosaccharide Specificity In Relation To Bacterial Adhesinsmentioning

confidence: 99%

Common oligosaccharide moieties inhibit the adherence of typical and atypical respiratory pathogens

Thomas

Brooks

2004

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Phenotypic and genotypic heterogeneity between strains, including differences between strains 08 and K96243 (24), is extensive in the microbial world and is well documented for B. pseudomallei (2,22,40,44,47). In this study, we report differences in microcolony formation, pilA expression, and biofilm formation between strains 08 and K96243; although the molecular basis for these differences is yet to be further delineated, they emphasize the need to consider virulence in B. pseudomallei to be due to a collection of factors distributed unequally between significantly different strains.…”

Section: Discussionmentioning

confidence: 99%

Temperature-Regulated Microcolony Formation byBurkholderia pseudomalleiRequirespilAand Enhances Association with Cultured Human Cells

Boddey¹,

Flegg²,

Day

et al. 2006

Infect Immun

View full text Add to dashboard Cite

Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease that is endemic to Northern Australia and Southeast Asia and is acquired from soil or water. Adherence of B. pseudomallei 08 to cultured cells increases dramatically following prior growth at 30°C or less compared to that following prior growth at 37°C. Here, we show that this occurs almost entirely as the result of microcolony formation (bacterium-bacterium interactions) following growth at 27°C but not at 37°C, which considerably enhances bacterial association with eukaryotic cells. Further, we demonstrate that the type IVA pilin-encoding gene, pilA, is essential for microcolony development by B. pseudomallei 08, and thus optimum association with eukaryotic cells, but is not required for direct adherence (bacterium-cell interactions). In contrast, although the B. pseudomallei genome sequence strain, K96243, also contains transcriptionally active pilA, microcolony formation rarely occurs following growth at either 27°C or 37°C and cell association occurs significantly less than with strain 08. Analysis of pilA transcription in 08 identified that pilA is dramatically upregulated under microcolony-forming conditions, viz., growth at low temperature, and association with eukaryotic cells; the pattern of transcription of pilA in K96243 differed from that in 08. Our study also suggests that biofilm formation by B. pseudomallei 08 and K96243 on polyvinylchloride is not mediated by pilA. Adherence and microcolony formation, and pilA transcription, vary between strains, consistent with known genomic variation in B. pseudomallei, and these phenotypes may be relevant to colonization from the environment.

show abstract

“…The increased adhesion correlated with subsequent increased invasion and cytopathic effect, but, as in many other studies, the molecular basis for the difference was not determined. Likewise, a study which inferred, through inhibition studies, the attachment of B. pseudomallei to the GM1–GM2 glycosphingolipid ganglioside on undefined primary human pharyngeal epithelial cells did not investigate the bacterial component(s) involved (Gori et al, 1999). …”

Section: The Adhesion Of B Pseudomallei To Host Cells: An Early Stepmentioning

confidence: 99%

Strategies for Intracellular Survival of Burkholderia pseudomallei

Allwood¹,

Devenish²,

Prescott³

et al. 2011

Front. Microbio.

105

112

View full text Add to dashboard Cite

Burkholderia pseudomallei is the causative agent of melioidosis, a disease with high mortality that is prevalent in tropical regions of the world. A key component of the pathogenesis of melioidosis is the ability of B. pseudomallei to enter, survive, and replicate within mammalian host cells. For non-phagocytic cells, bacterial adhesins have been identified both on the bacterial surface and associated with Type 4 pili. Cell invasion involves components of one or more of the three Type 3 Secretion System clusters, which also mediate, at least in part, the escape of bacteria from the endosome into the cytoplasm, where bacteria move by actin-based motility. The mechanism of actin-based motility is not clearly understood, but appears to differ from characterized mechanisms in other bacterial species. A small proportion of intracellular bacteria is targeted by host cell autophagy, involving direct recruitment of LC3 to endosomes rather than through uptake by canonical autophagosomes. However, the majority of bacterial cells are able to circumvent autophagy and other intracellular defense mechanisms such as the induction of inducible nitric oxide synthase, and then replicate in the cytoplasm and spread to adjacent cells via membrane fusion, resulting in the formation of multi-nucleated giant cells. A potential role for host cell ubiquitin in the autophagic response to bacterial infection has recently been proposed.

show abstract

Mediation of attachment of Burkholderia pseudomallei to human pharyngeal epithelial cells by the asialoganglioside GM1-GM2 receptor complex.

Cited by 18 publications

References 15 publications

Common oligosaccharide moieties inhibit the adherence of typical and atypical respiratory pathogens

Common oligosaccharide moieties inhibit the adherence of typical and atypical respiratory pathogens

Temperature-Regulated Microcolony Formation byBurkholderia pseudomalleiRequirespilAand Enhances Association with Cultured Human Cells

Strategies for Intracellular Survival of Burkholderia pseudomallei

Contact Info

Product

Resources

About