Mediation by Interleukin-1 of Neutrophil Leukocyte Emigration Induced by Endotoxin1

Cybulsky, Myron I.; McComb, Donna J.; Dinarello, Charles A.; Movat, Henry Z.

doi:10.1159/000414029

Cited by 31 publications

(42 citation statements)

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“…Thus, the occurrence of glands in various stages of transition to a lactating status provides a likely explanation for the variability of responses to ZAP in dexamethasone-treated ewes. Furthermore, these results are consistent with the conclusion that activation of ovine complement by endotoxin plays a trivial role in the induction of inflammatory responses to endotoxin (1).…”

Section: Discussionsupporting

confidence: 90%

“…In the skin of rabbits, for instance, as little as 10 ~'^ moles (12 pg) of endotoxin induces significant accumulation of neutrophils at the injection site (1); in the ovine mammary gland around 4x10"'^ moles (5 ng) of endotoxin is needed to provoke a response (2). Endotoxin is not chemotactic for leucocytes and must activate endogenous mediators in the host in order to stimulate the accumulation of neutrophils in inflammatory lesions (3).…”

Section: Introductionmentioning

confidence: 99%

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Induction of inflammatory responses by endotoxin in the non‐lactating ovine mammary gland

Colditz

1987

Immunol Cell Biol

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Summary. In non-lactating ewes, intramammary infusion of endotoxin provokes an intense but transient inflammatory response as assessed by mammary lavage. The variability of the method for sampling the leucocytes in mammary secretions was examined. Initial lavage with 20 ml pyrogen-free saline recovered 83-0± 2-8 and 84-4 ± 3-0% of the total leucocyte numbers harvested by three serial washes of quiescent and inflamed glands, respectively. Lavage of glands prior to infusion of endotoxin did not affect the magnitude of the subsequent inflammatory response. Three treatments at 3-5 day intervals with the glucocorticoid, dexamethasone, which inhibits the inflammatory response to intramammary infusion of antigen in systemically immunized ewes, did not affect the inflammatory response to endotoxin. However, the variability of the inflammatory response to activated complement was greatly increased in dexamethasone-treated ewes; this effect was attributed to the induction of lactation which results in loss of sensitivity of the gland to the inflammatory activity of activated complement. The results indicated that the macrophages and lymphocytes which are present in mammary secretions in large numbers, and dexamethasone-sensitive lymphocytes in the gland, played a trivial role in the induction of inflammatory responses to endotoxin.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Induction of inflammatory responses by endotoxin in the non‐lactating ovine mammary gland

Colditz

1987

Immunol Cell Biol

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“…Following an in¯ammatory response outside the brain, there is a rapid in®ltration of neutrophils and monocytes that occurs within minutes of the in¯ammatory stimulus. Peak migration rates for neutrophils and monocytes outside the brain typically occur within 4 h (Cybulsky et al, 1988). This is in stark contrast to the time course of in®ltration observed in the CNS following acute in¯ammatory responses.…”

Section: Role Of the Blood-brain Barrier In Traf®cking Of Immune Cellsmentioning

confidence: 85%

Immunobiology of the blood-brain barrier

Miller¹

1999

J Neurovirol

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The brain microvessel endothelial cells (BMVEC) that form the blood-brain barrier are uniquely positioned to in¯uence immune responses within the central nervous system. As the biological interface separating the blood from the brain extracellular¯uid, BMVEC regulate the entry of leukocytes into the brain. In addition, through the release of various soluble factors that affect immune responses, BMVEC may modulate immune responses in the brain. This review addresses the interplay between the immune system and the blood-brain barrier as it relates to the regulation of CNS defense and immunity.

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“…9,22,23) On the other hand, fMLP unlike LPS and PAF, recruits neutrophils to inflamed tissues by a direct mechanism independent of lipid mediator and protein synthesis. 15,24) N. procerum LAE inhibited both in vitro chemotaxis and in vivo pleural recruitment of neutrophils induced by PAF or fMLP. Therefore, the N. procerum LAE actual cell target within endotoxemic inflammation appears to be the neutrophils themselves, which have their motility machinery disrupted.…”

Section: Discussionmentioning

confidence: 95%

“…Neutrophil migration is a hallmark of the acute inflammatory process and is specially prominent after LPS challenge. 5,12,15,16) Following recognition by TLR4 and activation of TLR signaling pathways, LPS induces not only the production of prostanoids but also the production and release of an array of cytokines that are essential for the development of the subsequent inflammatory process.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Activity in the Aqueous Crude Extract of the Leaves of Nidularium procerum: A Bromeliaceae from the Brazilian Coastal Rain Forest

Amendoeira¹,

Frutuoso²,

Zanon³

et al. 2005

Biological & Pharmaceutical Bulletin

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Brazilian flora, especially in the Brazilian coastal rain forest, remains poorly studied regarding its chemical and pharmacological potential. A recent study published by our group indicates that Nidularium procerum, the most common representative of the bromeliaceae family, has potent analgesic and anti-inflammatory activities.1) Bromeliaceae, one of the largest botanical families of the New World, is extensively distributed in tropical America.2) This family comprises 46 genera encompassing about 2500 species from which 20 genera and 245 species are found in the State of Rio de Janeiro. The Brazilian coastal rain forest is the preferred habitat of Bromeliaceae (81.8%) where the greatest genetic diversity and the highest degree of endemism is found.3) Species of Nidularium are well spread in the coastal rain forest from Bahia State, in the northeast region of Brazil, to Rio Grande do Sul State in the south. 4)Our previous results indicate that N. procerum extracts have strong analgesic activity, which is not associated with the activation of opioid receptors, but is mechanistically due to a selective inhibition of PGE 2 production.1) In addition, we showed that the aqueous extract of N. procerum leaves (LAE) inhibited LPS-induced neutrophil accumulation in the mouse pleural cavity, but the molecular mechanism for this anti-inflammatory effect was not investigated.1) Here, we investigated the mechanisms involved in the anti-inflammatory effects of N. procerum aqueous extract on the LPS-induced pleurisy model. We showed that in addition to LPS-induced neutrophil influx, N. procerum also inhibited LPS-driven in vivo neutrophil activation (as attested by inhibition of lipid body formation) and PAF-and fMLP-induced pleural neutrophilia. The mechanisms involved in N. procerum-mediated inhibition of neutrophil recruitment appear to involve decreased production of key inflammatory mediators of LPSdriven response, such as PGE 2 and inflammatory cytokines (IL-1 and IL-6), and an impaired locomotory activity of migrating neutrophils. MATERIALS AND METHODS Plant MaterialNidularium procerum LINDMAN was collected at the National Biological Reserve of Poço das Antas, Silva Jardim, Rio de Janeiro State, Brazil. Identification of the botanical material was made by Dr. Tânia Wendt and Dr. Elton Leme and a voucher has been deposited at the Herbarium of the Rio de Janeiro Botanical Garden, under the number 304732.Preparation of Plant Extracts The aqueous extract of leaves (LAE) (22 g) was obtained by infusing dried leaves (500 g) into 2.5 l of boiling distilled water until the water cooled down to room temperature, followed by filtration and lyophilization as previously described. 1)Animals Male Swiss mice (obtained from the Oswaldo Cruz Foundation breeding unit) weighing 20-30 g, were used. The animals were maintained with free access to food and water and kept at 25-28°C with controlled 12 h light/dark cycle at the Department of Physiology and Pharmacodynamics. The experiments in this study received prior approval from the Oswal...

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Mediation by Interleukin-1 of Neutrophil Leukocyte Emigration Induced by Endotoxin1

Cited by 31 publications

References 0 publications

Induction of inflammatory responses by endotoxin in the non‐lactating ovine mammary gland

Induction of inflammatory responses by endotoxin in the non‐lactating ovine mammary gland

Immunobiology of the blood-brain barrier

Anti-inflammatory Activity in the Aqueous Crude Extract of the Leaves of Nidularium procerum: A Bromeliaceae from the Brazilian Coastal Rain Forest

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