Media for the detection and enumeration of Bacillus cereus in foods: a review

Netten, P. van; Kramer, John M.

doi:10.1016/0168-1605(92)90108-f

Cited by 63 publications

(39 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The factors that make B. cereus a potential threat to food processing is its ability to form thermoduric endospore, ability to grow and survive at refrigeration temperature and toxin production (Griffiths 1990;Van Netten and Kramer 1992;Granum and Lund 1997;McKillip 2000). Milk and rice perhaps are the two most commonly contaminated food items.…”

Section: B Cereus In Food and Food Productsmentioning

confidence: 99%

Bacillus cereus food poisoning: international and Indian perspective

2014

View full text Add to dashboard Cite

show abstract

Section: B Cereus In Food and Food Productsmentioning

confidence: 99%

Bacillus cereus food poisoning: international and Indian perspective

2014

View full text Add to dashboard Cite

show abstract

“…Some Bacillus spp., especially Bacillus cereus, are ␤-D-glucosi- dase, PI-PLC, and PC-PLC positive (27,29,30); thus, they could grow on chromogenic media and produce colonies similar to those of L. monocytogenes (24,30). Therefore, it is necessary to inhibit growth of B. cereus to ensure the specificity of the selective medium.…”

Section: Discussionmentioning

confidence: 99%

“…However, chromogenic media can be expensive and may not be suitable for routine laboratory use (22). Also some Bacillus spp., especially Bacillus cereus, and Staphylococcus aureus are ␤-D-glucosidase, PI-PLC, and PC-PLC positive, and thus, they could grow on chromogenic media and produce colonies similar to those of L. monocytogenes (22,(26)(27)(28)(29)(30).…”

mentioning

confidence: 99%

Development of a Novel Selective and Differential Medium for the Isolation of Listeria monocytogenes

Park

Chang

Ryu

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

A new medium (lecithin and levofloxacin [LL] medium) is described for the isolation of Listeria monocytogenes from food samples. LL medium includes lecithin from soybeans for the detection of phosphatidylinositol-specific phospholipase C (PI-PLC) and phosphatidylcholine-specific phospholipase C (PC-PLC) produced by L. monocytogenes. Levofloxacin is incorporated to inhibit the growth of microorganisms other than L. monocytogenes, especially Bacillus cereus, shown to possess PI-PLC and PC-PLC activities. L. monocyogenes produced white colonies with a halo on LL medium, whereas Listeria innocua appeared as white colonies without a halo. Levofloxacin at 0.20 mg/liter completely inhibited the growth of B. cereus, while the growth of L. monocytogenes was unaffected. In the second phase of the study, the sensitivity and the specificity of LL medium were compared to those of modified Oxford agar (MOX) and two chromogenic media (Brilliance Listeria agar and CHROMagar Listeria), using a total of 250 food samples. From 200 unspiked food samples, the specificity of LL medium (96.0%) was superior to that of MOX (72.0%) and similar to the specificities of Brilliance Listeria agar (96.5%) and CHROMagar Listeria (94.5%). From 50 spiked food samples, LL medium and CHROMagar Listeria represented the highest sensitivities (96.0%), followed by Brilliance Listeria agar (92.0%) and MOX (54.0%). Also, LL medium showed the highest confirmation rate (98.8%), followed by Brilliance Listeria agar (98.7%), CHROMagar Listeria (98.3%), and MOX (52.0%). On the basis of its good specificity and cost effectiveness, LL medium is useful for the isolation of L. monocytogenes from food samples.

show abstract

“…Several selective culture-based techniques are available for the detection and enumeration of L. monocytogenes, B. cereus, and C. perfringens in environmental and food samples; and the international standard methods for the detection and enumeration of these food pathogens are based on cultivation [77,417]. The cultivation and subsequent identification of isolates using conventional techniques are time-consuming.…”

Section: Cultivationmentioning

confidence: 99%

Methodologies for the characterization of microbes in industrial environments: a review

Maukonen¹,

Mättö²,

Wirtanen³

et al. 2003

Journal of Industrial Microbiology and Biotechnology

121

View full text Add to dashboard Cite

There is growing interest in research and development to develop novel tools to study, detect, and characterize microbes and their communities in industrial environments. However, knowledge about their validity in practical industrial use is still scarce. This review describes the advantages and limitations of traditional and molecular methods used for biofilm and/or planktonic cell studies, especially those performed with Listeria monocytogenes, Bacillus cereus, and/or Clostridium perfringens. In addition, the review addresses the importance of isolating the microorganisms from the industrial environment and the possibilities and future prospects for exploiting the described methods in the industrial environment.

show abstract

Media for the detection and enumeration of Bacillus cereus in foods: a review

Cited by 63 publications

References 15 publications

Bacillus cereus food poisoning: international and Indian perspective

Bacillus cereus food poisoning: international and Indian perspective

Development of a Novel Selective and Differential Medium for the Isolation of Listeria monocytogenes

Methodologies for the characterization of microbes in industrial environments: a review

Contact Info

Product

Resources

About