Media and Fermentation Processes for the Rapid Production of High Concentrations of Stable Blastospores of the Bioinsecticidal Fungus<i>Paecilomyces fumosoroseus</i>

Jackson, Mark A.; Cliquet, Sophie; Iten, Loren B.

doi:10.1080/0958315021000054368

Cited by 62 publications

(22 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…After 30 h of submerged fermentation, we obtained a high number of viable blastospores and this type of propagules colonizes the substrate rapidly due to a faster germination rate than aerial conidia. Jackson et al (2003) observed more than 90% blastospores of I. fumosorosea after 6 h of incubation, while conidia required 16 h of incubation to achieve this germination rate. The solid substrates which resulted in the highest conidia productions were BC and SM for ESALQ1205 and WR for ESALQ1296 (p<0.0001).…”

Section: Resultsmentioning

confidence: 99%

Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

Mascarin

Alves

Lopes

2010

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

Mascarin

Alves

Lopes

2010

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…When blastospore production media derived from these concentrates were compared to blastospore production media prepared using our standard protocol, both media yielded blastospore concentrations of 1.1·10 9 blastospores/ml after 2 days growth [10]. Media concentrates stored at room temperature for 1 month showed no loss in blastospore production capability (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Liquid-culture production of blastospores of the bioinsecticidal fungus Paecilomyces fumosoroseus using portable fermentation equipment

Jackson

Payne

Odelson³

2004

J IND MICROBIOL BIOTECHNOL

Self Cite

View full text Add to dashboard Cite

The production of fungal spores using on-site, non-sterile, portable fermentation equipment is technically constrained. Very little information is available on the production requirements, such as medium concentration, inoculum stabilization, required fermentation times, and maintenance of axenic growth. In this study, we developed a two-part, liquid concentrate of the production medium that remains stable and soluble at room temperature. We also examined inoculum stability and showed that freeze- or air-dried blastospore preparations were stable for 7 days after rehydration when stored at 4 degrees C. The use of a low-pH (pH 4), relatively rich complex medium provided a growth environment deleterious to bacterial growth yet conducive to rapid sporulation by Paecilomyces fumosoroseus. High concentrations of blastospores (7.9 x 10(8)/ml) of P. fumosoroseus were produced in a 40-h fermentation with very low levels of bacterial contamination when the fermentor was charged with a blastospore production medium with a starting pH of 4 and inoculated with blastospore concentrations greater than 1 x 10(6) spores/ml. These studies demonstrate that the use of disinfected, portable fermentation equipment has potential for on-site production of high concentrations of blastospores of the bioinsecticidal fungus P. fumosoroseus.

show abstract

“…Para mejorar la producción, rendimiento y estabilidad de blastosporas de Isaria fumosorosea (Wise) (Brown & Smith) tolerantes a la desecación, Jackson et al (2003) realizaron una comparación de medios de cultivo líquido suplementados con fuentes de nitrógeno complejas (caseína hidrolizada con ácido o extracto de levadura) obteniendo altas concentraciones de blastosporas (1x10 9 blastosporas/ml; con 79-82% de supervivencia después del secado) en comparación con los producidos con otras fuentes de nitrógeno (12-50% de supervivencia después del secado).…”

Section: Introductionunclassified

Estudio de las condiciones de mezclado en fermentador para la producción de blastosporas de Beauveria bassiana

García-Gutiérrez¹,

González-Maldonado²,

Medrano-Roldán³

et al. 2013

Rev. Colomb. Biotecnol.

View full text Add to dashboard Cite

Estudio de las condiciones de mezclado en fermentador para la producción de ResumenSe caracterizaron tres fermentadores: New Brunswick M-19 de 14 litros, Applikon Biocontroller 1035 de 7 litros y New Brunswick Bioflo III de 7 litros, determinando el coeficiente volumétrico de transferencia de oxígeno (KLa), la retención de gas (RG) y el tiempo de mezclado (tM). El fermentador New Brunswick Bioflo III tuvo los mejores valores con una relación de diámetro del impulsor/diámetro del tanque (DI/DT) de 0.43, KLa = 9.5-208 h -1 y tM = 1.0-3.0 s, por lo que fue seleccionado para realizar la producción de blastosporas de Beauveria bassiana, utilizando melaza como fuente de carbono. Se estudiaron las condiciones de mezclado, utilizando diferentes combinaciones de impulsores tipo Rushton, Maxflo y Lightnin, bajo un diseño experimental factorial 3 2 . El tiempo de propagación fue de 4 días, el volumen de trabajo 4 litros, 10% de inóculo (1x10 6 blastosporas/ml), temperatura 30°C, agitación de 400-500 rpm, aireación de 0.5-1.0 vvm, y pH de 5.4.El hongo se desarrolló mejor utilizando la combinación de impulsores Rushton-Maxflo a 400 rpm y 1.0 vvm (F = 10.324, p ≤ 0.0123) (DMS=0.585), obteniendo una concentración de 1.2x10 9 blastosporas/ml, 2.2 g/l de biomasa y 2.48 g/l de consumo de sustrato (Y x/s=0.89). Las condiciones de mezclado y los parámetros obtenidos pueden ser aplicados en otros fermentadores para optimizar la producción de blastosporas de B. bassiana en la elaboración experimental de bioinsecticidas.Palabras clave: fermentación líquida, aireación, agitación, hongo entomopatógeno. AbstractIn this work three fermenters were characterized: New Brunswick M-19 of 14 liters, Biocontroller Applikon 1035 of 7 liters and New Brunswick Bioflo III of 7 liters, determining the volumetric coefficient oxygen transfer (KLa), gas hold up (GH) and the mixing time (tM). The fermenter New Brunswick Bioflo III had the best values with a ratio of diameter of the impeller/ vessel (DI/DT) of 0.43, KLa = 9.5-208 h -1 and tM = 1.0-3.0 s, so it was selected for the production of blastospores of Beauveria bassiana, using molasses as carbon source. We studied the mixing conditions, using different combinations of impellers, type Rushton, Lightnin and Maxflo under a factorial experimental design 3 2 . The propagation time was of 4 days, working volume 4l, 10% inoculum (1x10 6 blastospores/ml), temperature 30°C, agitation of 400-500 rpm, aeration 0.5-1.0 vvm, and pH of 5.4. The fungus growth better using a combination of impellers Rushton-Maxflo at 400 rpm and 1.0 vvm (F = 10.324, p ≤ 0.0123) (LSD = 0.585) obtaining a concentration of 1.2x10 9 blastospores/ml, 2.2 g/l biomass and 2.48 g/l of substrate consumption (Y x/s = 0.89). The mixing conditions and the parameters obtained can be applied to optimize the blastospore productions of B. bassiana to fermenter level in the experimental production of bioinsecticides.

show abstract

Media and Fermentation Processes for the Rapid Production of High Concentrations of Stable Blastospores of the Bioinsecticidal FungusPaecilomyces fumosoroseus

Cited by 62 publications

References 15 publications

Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

Liquid-culture production of blastospores of the bioinsecticidal fungus Paecilomyces fumosoroseus using portable fermentation equipment

Estudio de las condiciones de mezclado en fermentador para la producción de blastosporas de Beauveria bassiana

Contact Info

Product

Resources

About