Medaka igf1 identifies somatic cells and meiotic germ cells of both sexes

Yuan, Can‐Can; Chen, Kerang; Zhu, Yefei; Yuan, Yusheng; Li, Mingyou

doi:10.1016/j.gene.2017.11.037

Cited by 25 publications

(13 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with the results indicated in the RNA-seq data, however, RT-PCR and qPCR results revealed that the exepression of circ_024949, circ_026259 and IGF1 decreased gradually with development from 3-month-old to 3-year-old testes, suggesting that their expression negatively correlated with oar-miR-29b. The subsequent in situ hybridization analysis revealed that circ_024949, circ_026259, oar-miR-29b and IGF1 were all located in various germ cells, Leydig cells and Sertoli cells, which was largely consistent with a previous report on the subcellular localization patterns of testicular IGF1 mRNA (Yuan et al, 2018). This further implied the interactions between either circ_024949, circ_026259, or IGF1 and oar-miR-29b, as well as their potential modulation for the development of testicular germ and somatic cells.…”

Section: Discussionsupporting

confidence: 89%

Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development

Luo

Wang

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Circular RNAs (circRNAs) have been shown to function in the reproductive systems including testis. However, their expression, as well as function in testicular development of sheep remain undefined. Herein, we performed RNA sequencing to reveal circRNA temporal expression patterns in testes of Tibetan sheep from different stages of maturation (3M, 3-month-old; 1Y, 1-year-old; 3Y, 3-year-old). A total of 3,982, 414, and 4,060 differentially expressed (DE) circRNAs were uncovered from 3M vs 1Y, 1Y vs 3Y, and 3M vs 3Y, respectively. Functional enrichment assessment indicated that the source genes of DE circRNAs were primarily engaged in spermatogenesis and testicular immune privilege including blood–testis barrier (BTB). We subsequently constructed the core circRNA–miRNA–mRNA interaction network for genes related to testicular function, such as spermatogenesis, germ cell development, BTB, and cell cycle/meiosis. Furthermore, we validated the target associations between either circ_024949, circ_026259 or IGF1, and oar-miR-29b in this network, and revealed their similar expression signatures in developmental testes that they were extensively expressed in germ cells, Leydig cells, and Sertoli cells, thus suggesting their broad functions in the functional maintenance of Leydig cells and Sertoli cells, as well as the development and maturation of male germ cells. Meanwhile, circ_026259 was shown to promote IGF1 expression through inhibition of oar-miR-29b in sheep Sertoli cells. This work gives the first global view for the expression and regulation of circRNAs in sheep testis, which will be helpful for providing new insights into the molecular mechanism of ovine testis function.

show abstract

Section: Discussionsupporting

confidence: 89%

Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development

Luo

Wang

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…The developing stages of the ovary were determined based on the original definition as described previously [ 25 ]. The ovaries were dissected from the anesthetized female medaka.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the primordial germ cells specification [ 21 ], migration [ 22 ], and sex-determination mechanism [ 23 , 24 ] have been systematically explored. Previously, we have investigated that igf1 is present in meiotic germ cells and somatic cells of both sexes in medaka [ 25 ]. Igf2 is associated with self-renewal of the embryonic stem cell [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of Duplicate Insulin-like Growth Factor-1 Receptors (igf1rs) in Medaka Gonads

Wei

Zhu

Yuan

et al. 2022

Life

Self Cite

View full text Add to dashboard Cite

Insulin-like growth factor-1 receptors (igf1rs) play important roles in regulating development, differentiation, and proliferation in diverse organisms. In the present study, subtypes of medaka igf1r, igf1ra, and igf1rb were isolated and characterized. RT-PCR results showed that igf1ra and igf1rb mRNA were expressed in all tissues and throughout embryogenesis. Using real-time PCR, the differential expression of igf1ra and igf1rb mRNA during folliculogenesis was observed. The results of in situ hybridization (ISH) revealed that both of them were expressed in ovarian follicles at different stages, and igf1rb was also expressed in theca cells and granulosa cells. In the testis, both igf1ra and igf1rb mRNA were highly expressed in sperm, while igf1rb mRNA was also obviously detected in spermatogonia. In addition, igf1ra mRNA was also present in Leydig cells in contrast to the distribution of igf1rb mRNA in Sertoli cells. Collectively, we demonstrated that differential igf1rs RNA expression identifies medaka meiotic germ cells and somatic cells of both sexes. These findings highlight the importance of the igf system in the development of fish gonads.

show abstract

“…Section in situ hybridisation (SISH) and fluorescent in situ hybridisation (FISH) were performed as described (Li et al, ; Yuan et al, ). The open reading frames (ORF) of vasa was inserted into the pGEM‐T vector to get a plasmid (pT Clvasa ).…”

Section: Methodsmentioning

confidence: 99%

Evolutionarily conserved vasa identifies embryonic and gonadal germ cells in spinyhead croaker Collichthys lucidus

Liu

Song

Zhu

et al. 2019

Journal of Fish Biology

View full text Add to dashboard Cite

In this study, a 2198 bp full-length cDNA of spinyhead croaker Collichthys lucidus vasa gene encoding 616 amino-acid residues was obtained. Multiple alignment revealed that C. lucidus vasa has eight conserved characteristic motifs of the DEAD box protein family and has the highest identity to large yellow croaker Larimichthys croceas. Reverse-transcription (RT)-PCR and Western blot analyses indicated that the vasa messenger (m)RNA and Vasa protein are specifically expressed in the gonads in both sexes. In situ hybridisation (ISH) demonstrated that vasa RNA is exclusively detected in the germ cells in C. lucidus gonads and its temporospatial expression reveals a dynamic pattern during oogenesis. Surprisingly, C. lucidus vasa 3'UTR can direct stable and specific GFP expression in the primordial germ cells (PGC) of medaka Oryzias latipes embryos. Taken together, these results suggest that because C. lucidus vasa expression delineates critical stages of oogenesis, it may be a useful molecular marker for the identification of gonadal germ cells, facilitating the isolation and utilization of germ cells in future study.

show abstract

Medaka igf1 identifies somatic cells and meiotic germ cells of both sexes

Cited by 25 publications

References 41 publications

Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development

Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development

Differential Expression of Duplicate Insulin-like Growth Factor-1 Receptors (igf1rs) in Medaka Gonads

Evolutionarily conserved vasa identifies embryonic and gonadal germ cells in spinyhead croaker Collichthys lucidus

Contact Info

Product

Resources

About