MED30 Regulates the Proliferation and Motility of Gastric Cancer Cells

Lee, Yong Joo; Han, Myoung‐Eun; Baek, Su‐Jin; Kim, Seon‐Young; Oh, Sae‐Ock

doi:10.1371/journal.pone.0130826

Cited by 9 publications

(14 citation statements)

References 33 publications

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“…Tissue microarray sections containing pancreatic cancer tissues from patients were obtained from SuperBiochip (Seoul). Histopathologic diagnoses were performed as described before [14]. All patients had histologically con rmed pancreatic cancer, and tumor samples were checked to ensure that tumor tissue composed more than 80% of samples.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…All patients had histologically con rmed pancreatic cancer, and tumor samples were checked to ensure that tumor tissue composed more than 80% of samples. Remaining procedures were performed as described before [14]. siRNA transfection SCR and MED30 siRNAs were purchased from Dharmacon (Lafayette, CO, USA) and Bioneer (Daejeon, Korea), respectively.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Pancreatic cancer cells were seeded at 2000 cells/well in 96-well plates, or 8×10 4 cells/well in 6-well plates. Cell transfection with MED30 siRNA was performed as described before [14] Plasmid DNA construction and generation of stable cell lines…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…To examine the FBS-induced cell invasion, we used 24-well BioCoatTMMatrigel chamber inserts (BD Bioscience, San Jose, CA, USA). The remaining procedures of Matrigel invasion assay were performed as described before [14] Soft-agar assay Knockdown or MED30-overexpressing PANC-1cells were suspended in media containing 0.25% agar and overlaid on 0.5% agar in 6-well plates. After 2 weeks, colonies were stained with 0.005% crystal violet in 10% methanol and photographed.…”

Section: Matrigel Invasion Assaymentioning

confidence: 99%

“…MED30 homozygous mice showed its roles in regulating mitochondrial functions and integrity [13]. Recently, MED30 has been reported to regulate the proliferation and motility of gastric cancer cells [14]. In the presentstudy, we examined the expression and roles of MED30 in pancreatic cancer.…”

Section: Introductionmentioning

confidence: 99%

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The Prognostic Significance and Tumor-promoting Roles of MED30 in Pancreatic Cancer

Liu

Han

Kim

et al. 2020

Preprint

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Background: MED30 is an evolutionarily new member of the mediator complex thatcan bridge transcription factors and preinitiation complexfor the transcription. Pancreatic cancer is highly chemo-resistant andis the fourth leading cause of cancer-related deaths. Rapid progression and poor prognosis of pancreatic cancer result in shortened survival rate following diagnosis.Methods: Expression of MED30 was investigated by the analysis of TCGA database and immunohistochemistry of patients tissues. Roles of MED30 were investigated by gain-of-function and loss-of-function studies.Results: Analysis of TCGA database and immunohistochemistry provedthe frequentamplification and overexpression of MED30 in the pancreatic cancer tissues compared with the normal pancreatic tissues at the chromosome, mRNA and protein levels. Notably its overexpression was association with poor prognosis of pancreatic cancer patients in the Kaplan-Meier curve analysis (P=0.00072) and multivariate analysis (P=0.027). Uno’s C-index values in the time-dependent Area Under the Curve (AUC) analysis and AUC valuein the receiver operating characteristics (ROC) curves showed its good performance as a prognostic marker. To reveal the functional roles of MED30 during the progression of pancreatic cancer, we overexpressed or knocked down MED30 using cDNA or siRNA, respectively. Gain-of-function and loss-of-function studies showed that MED30 can regulate proliferation, migration, and invasion of pancreatic cancer cells. Furthermore overexpression of MED30 promoted tumorigenicity in SCID mice significantly. Conclusions: MED30 is frequently amplified and overexpressed and its overexpression is associated with poor prognosis of pancreatic cancer patients. Moreover it has tumor-promoting roles in proliferation, migration, invasion and in vivo tumorigenicity of pancreatic cancer cells. Thus, MED30 could be a potent diagnostic and therapeutic target in pancreatic cancer.

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Section: Immunohistochemistrymentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Matrigel Invasion Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Prognostic Significance and Tumor-promoting Roles of MED30 in Pancreatic Cancer

Liu

Han

Kim

et al. 2020

Preprint

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Gene signature predicting recurrence in oral squamous cell carcinoma is characterized by increased oxidative phosphorylation

et al. 2022

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Although numerous studies have used systemic approaches to identify prognostic predictors in oral squamous cell carcinoma (OSCC), the effectiveness of these approaches has not been assessed clinically. Further, the mechanism underlying malignant behaviors in OSCC is poorly characterized. This study aimed to develop and verify accurate prognostic predictors for OSCC patients and assess the associated biology. We identified an OSCC‐recurrence‐related gene signature (ORGS) using a Cox regression analysis. Functional enrichment analysis was used to identify enriched pathways and biological processes to reveal the underlying mechanism of OSCC malignant behavior. The ORGS successfully divided OSCC patients into low‐ and high‐risk groups with significantly different overall survivals. Pathway analysis revealed oxidative phosphorylation (OXPHOS) as a signaling pathway associated with the ORGS in OSCC. Interestingly, high OXPHOS status was strongly associated with poor overall survival in OSCC patients. Mediator complex subunit 30 (MED30) was a predicted upstream regulator of OXPHOS, and knockdown of MED30 reduced histone acetylation. We identified that the ORGS was strongly correlated with OXPHOS regulatory processes, suggesting OXPHOS as a key mechanism leading to poor prognosis in OSCC.

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MED28 and forkhead box M1 (FOXM1) mediate matrix metalloproteinase 2 (MMP2)‐dependent cellular migration in human nonsmall cell lung cancer (NSCLC) cells

Hsieh

Huang

et al. 2018

Journal Cellular Physiology

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Non‐small‐cell lung cancer (NSCLC) accounts for the majority of the lung cancer cases that have become a leading cause of cancer deaths worldwide. Overexpression of transcription factor forkhead box M1 (FOXM1) is involved in the inauspicious development of several types of cancer, including lung tumor aggressiveness. Our laboratory has previously found that MED28, a Mediator subunit for transcriptional activation, modulates cell growth, epithelial‐mesenchymal transition, migration, and invasion in human breast cancer cells. The objective of the current study is to investigate the potential role of MED28 and FOXM1 in NSCLC. In addition to A549 and PC9 cells, we also used a doxycycline‐inducible system to generate FOXM1‐overexpressed A549‐DN cells, and we explored the connection of MED28 with FOXM1 and their effect on migration. Herein, we report that the increased expression levels of both MED28 and FOXM1 elevated the expression of matrix metalloproteinase 2 (MMP2), a metastasis marker, which enhanced cell migration and matrigel invasion of NSCLC cells. Furthermore, MED28 interacted with FOXM1, and both exhibited a mutual effect on the expression and subcellular localization. Moreover, MED28 small interfering RNA‐mediated MMP2 gene suppression could be attenuated by inducible expression of a constitutively active form of FOXM1, which consequently restored the migration and invasion ability of NSCLC cells. Our data indicate that MED28 interacts with FOXM1, and each affects the expression and localization of the other, and, more importantly, both regulate MMP2‐dependent migration and invasion in human lung cancer cells.

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MED30 Regulates the Proliferation and Motility of Gastric Cancer Cells

Cited by 9 publications

References 33 publications

The Prognostic Significance and Tumor-promoting Roles of MED30 in Pancreatic Cancer

The Prognostic Significance and Tumor-promoting Roles of MED30 in Pancreatic Cancer

Gene signature predicting recurrence in oral squamous cell carcinoma is characterized by increased oxidative phosphorylation

MED28 and forkhead box M1 (FOXM1) mediate matrix metalloproteinase 2 (MMP2)‐dependent cellular migration in human nonsmall cell lung cancer (NSCLC) cells

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