Meconium Microbiome of Very Preterm Infants across Germany

Klopp, Jonas; Ferretti, Pamela; Meyer, Claudius U.; Hilbert, Katja; Haiß, Annette; Marißen, Janina; Henneke, Philipp; Hudalla, Hannes; Pirr, Sabine; Viemann, Dorothee; Zemlin, Michael; Forslund, Kristoffer; Härtel, Christoph; Bork, Peer; Gehring, Stephan; Rossum, Thea Van

doi:10.1128/msphere.00808-21

Cited by 22 publications

(26 citation statements)

References 61 publications

(62 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Meconium: Birthmentioning

confidence: 99%

“…microbiota normally exhibits large between-individual variation, which is influenced by gestational age at birth (Klopp et al, 2022). At the phylum level, the complexity of meconium microbiota is low in most cases with a single phylum, such as Firmicutes, Bacteroidetes, and Proteobacteria, as the most predominant ones (Gómez et al, 2017;Hiltunen et al, 2021;Klopp et al, 2022).…”

Section: Meconium: Birthmentioning

confidence: 99%

Section: Development Of Gut Microbiota In Preterm Birthmentioning

confidence: 99%

“…Although the idea of whether the prenatal environment is sterile remains questionable ( Fricke and Ravel, 2021 ), the presence of microbiota has been detected in meconium by using either next-generation sequencing (NGS) or real-time PCR ( Morais et al, 2020 ; Hiltunen et al, 2021 ; Klopp et al, 2022 ). The meconium microbiota normally exhibits large between-individual variation, which is influenced by gestational age at birth ( Klopp et al, 2022 ). At the phylum level, the complexity of meconium microbiota is low in most cases with a single phylum, such as Firmicutes, Bacteroidetes, and Proteobacteria, as the most predominant ones ( Gómez et al, 2017 ; Hiltunen et al, 2021 ; Klopp et al, 2022 ).…”

Section: Development Of Gut Microbiota In Preterm Birthmentioning

confidence: 99%

“…The meconium microbiota normally exhibits large between-individual variation, which is influenced by gestational age at birth ( Klopp et al, 2022 ). At the phylum level, the complexity of meconium microbiota is low in most cases with a single phylum, such as Firmicutes, Bacteroidetes, and Proteobacteria, as the most predominant ones ( Gómez et al, 2017 ; Hiltunen et al, 2021 ; Klopp et al, 2022 ). The Bifidobacterium , Staphylococcus , Enterococcus , Streptococcus , and Escherichia are the most abundant genera ( Klopp et al, 2022 ), and these are comparable to full-term infants except for the genus Bacteroides which is present with high abundance in full-term infants ( Yassour et al, 2018 ; Wang et al, 2021 ).…”

Section: Development Of Gut Microbiota In Preterm Birthmentioning

confidence: 99%

See 4 more Smart Citations

First 1000 Days and Beyond After Birth: Gut Microbiota and Necrotizing Enterocolitis in Preterm Infants

Zeng

Ying²,

Li³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Preterm birth remains a major maternal and infant health issue worldwide particularly with an increase in the global preterm birth rate, which requires more interventions to manage the consequences of preterm birth. In addition to traditional complications, recent studies have shown that the succession of gut microbiota of preterm infants is disordered due to the systemic physiological immaturity, which confers negative influences on the growth, development, and health of infants. In the present study, we briefly discussed the prevalence of preterm birth worldwide and then highlighted the signatures of gut microbiota in preterm infants within the first 1000 days of life after the birth categorized into birth, infancy, and childhood. Afterward, we focused on the potential association of clinical phenotypes typically associated with preterm birth (i.e., necrotizing enterocolitis) with gut microbiota, and the potential directions for future studies in this field are finally discussed.

show abstract

Section: Meconium: Birthmentioning

confidence: 99%

Section: Meconium: Birthmentioning

confidence: 99%

Section: Development Of Gut Microbiota In Preterm Birthmentioning

confidence: 99%

Section: Development Of Gut Microbiota In Preterm Birthmentioning

confidence: 99%

Section: Development Of Gut Microbiota In Preterm Birthmentioning

confidence: 99%

See 3 more Smart Citations

First 1000 Days and Beyond After Birth: Gut Microbiota and Necrotizing Enterocolitis in Preterm Infants

Zeng

Ying²,

Li³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

Bifidobacterium and Lactobacillus Probiotics and Gut Dysbiosis in Preterm Infants

Van Rossum,

Haiß,

Knoll

et al. 2024

JAMA Pediatr

View full text Add to dashboard Cite

ImportanceThe effects of probiotic interventions on colonization with resistant bacteria and early microbiome development in preterm infants remain to be clarified.ObjectiveTo examine the efficacy of Bifidobacterium longum subsp infantis, Bifidobacterium animalis subsp lactis (BB-12), and Lactobacillus acidophilus (La-5) probiotics to prevent colonization with multidrug-resistant organisms or highly epidemic bacteria (MDRO+) and to shape the microbiome of preterm infants toward the eubiotic state of healthy full-term infants.Design, Setting, and ParticipantsThe multicenter, double-blinded, placebo-controlled, group sequential, phase 3 Priming Immunity at the Beginning of Life (PRIMAL) randomized clinical trial, conducted from April 2018 to June 2020, included infants with gestational age of 28 to 32 weeks at 18 German neonatal units. Data analyses were conducted from March 2020 to August 2023.InterventionA total of 28 days of multistrain probiotics diluted in human milk/formula starting within the first 72 hours of life.Main Outcomes and MeasuresColonization with MDRO+ at day 30 of life (primary end point), late-onset sepsis and severe gastrointestinal complication (safety end points), and gut dysbiosis, ie, deviations from the microbiome of healthy, term infants (eubiosis score) based on 16-subunit ribosomal RNA and metagenomic sequencing.ResultsAmong the 643 infants randomized until the stop of recruitment based on interim results, 618 (median [IQR] gestational age, 31.0 [29.7-32.1] weeks; 333 male [53.9%]; mean [SD] birth weight, 1502 [369] g) had follow-up at day 30. The interim analysis with all available data from 219 infants revealed MDRO+ colonization in 43 of 115 infants (37.4%) in the probiotics group and in 39 of 104 infants (37.5%) in the control group (adjusted risk ratio, 0.99; 95% CI, 0.54-1.81; P = .97). Safety outcomes were similar in both groups, ie, late-onset sepsis (probiotics group: 8 of 316 infants [2.5%]; control group: 12 of 322 infants [3.7%]) and severe gastrointestinal complications (probiotics group: 6 of 316 infants [1.9%]; control group: 7 of 322 infants [2.2%]). The probiotics group had higher eubiosis scores than the control group at the genus level (254 vs 258 infants; median scores, 0.47 vs 0.41; odds ratio [OR], 1.07; 95% CI, 1.02-1.13) and species level (96 vs 83 infants; median scores, 0.87 vs 0.59; OR, 1.28; 95% CI, 1.19-1.38). Environmental uptake of the B infantis probiotic strain in the control group was common (41 of 84 [49%]), which was highly variable across sites and particularly occurred in infants with a sibling who was treated with probiotics.Conclusions and RelevanceMultistrain probiotics did not reduce the incidence of MDRO+ colonization at day 30 of life in preterm infants but modulated their microbiome toward eubiosis.Trial RegistrationGerman Clinical Trials Register: DRKS00013197

show abstract

Shaping Microbiota During the First 1000 Days of Life

Samarra,

Flores,

Bernabeu

et al. 2024

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Meconium Microbiome of Very Preterm Infants across Germany

Abstract: Meconium is present in the intestines of infants before and after birth and constitutes their first bowel movements postnatally. The consistency, composition and microbial load of meconium is largely different from infant and adult stool.

Cited by 22 publications

References 61 publications

First 1000 Days and Beyond After Birth: Gut Microbiota and Necrotizing Enterocolitis in Preterm Infants

First 1000 Days and Beyond After Birth: Gut Microbiota and Necrotizing Enterocolitis in Preterm Infants

Bifidobacterium and Lactobacillus Probiotics and Gut Dysbiosis in Preterm Infants

Shaping Microbiota During the First 1000 Days of Life

Contact Info

Product

Resources

About