Mechanosynthesis of hydroxyapatite–ferrite composite nanopowder

Власова, М.; Fedotov, A. Yu.; Torrez, I. Mendoza; Kakazey, M.; Komlev, V. S.; Aguilar, P.A. Márquez

doi:10.1016/j.ceramint.2017.02.020

Cited by 8 publications

(3 citation statements)

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“… 22 , 57 , 58 So far, several attempts have been made to combine hydroxyapatite and IO, especially into the core/shell system to utilise it within biomedical fields, eg, tissue engineering and regenerative medicine. 59 , 60 This work was focused on the nHAp/IO composite, which was successfully obtained by the wet chemistry method. It was found that fabricated nHAp/IO consists of two separate well-crystalised phases (with the content of 68%/32%, respectively) on the nanometric scale with a tendency to mutual agglomeration.…”

Section: Discussionmentioning

confidence: 99%

Nanohydroxyapatite (nHAp) Doped with Iron Oxide Nanoparticles (IO), miR-21 and miR-124 Under Magnetic Field Conditions Modulates Osteoblast Viability, Reduces Inflammation and Inhibits the Growth of Osteoclast – A Novel Concept for Osteoporosis Treatment: Part 1

Marycz

Śmieszek

Marcinkowska

et al. 2021

IJN

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Purpose: Osteoporosis results in a severe decrease in the life quality of many people worldwide. The latest data shows that the number of osteoporotic fractures is becoming an increasing international health service problem. Therefore, a new kind of controllable treatment methods for osteoporotic fractures is extensively desired. For that reason, we have manufactured and evaluated nanohydroxyapatite (nHAp)-based composite co-doped with iron oxide (IO) nanoparticles. The biomaterial was used as a matrix for the controlled delivery of miR-21-5p and miR-124-3p, which have a proven impact on bone cell metabolism. Methods: The nanocomposite Ca 5 (PO 4 ) 3 OH/Fe 3 O 4 (later called nHAp/IO) was obtained by the wet chemistry method and functionalised with microRNAs (nHAp/IO@miR-21/124). Its physicochemical characterization was performed using XRPD, FT-IR, SEM-EDS and HRTEM and SAED methods. The modulatory effect of the composite was tested in vitro using murine pre-osteoblasts MC3T3-E1 and pre-osteoclasts 4B12. Moreover, the anti-inflammatory effects of biomaterial were analysed using a model of LPS-treated murine macrophages RAW 264.7. We have analysed the cells' viability, mitochondria membrane potential and oxidative stress under magnetic field (MF+) and without (MF-). Moreover, the results were supplemented with RT-qPCR and Western blot assays to evaluate the expression profile for master regulators of bone metabolism. Results: The results indicated pro-osteogenic effects of nHAp/IO@miR-21/124 composite enhanced by exposure to MF. The enhanced osteogenesis guided by nHAp/IO@miR-21/124 presence was associated with increased metabolism of progenitor cells and activation of osteogenic markers (Runx-2, Opn, Coll-1). Simultaneously, nanocomposite decreased metabolism and differentiation of pre-osteoclastic 4B12 cells accompanied by reduced expression of CaII and Ctsk. Obtained composite regulated viability of bone progenitor cells and showed immunomodulatory properties inhibiting the expression of inflammatory markers, ie, TNF-α, iNOs or IL-1β, in LPS-stimulated RAW 264.7 cells. Conclusion:We have described for the first time a new concept of osteoporosis treatment based on nHAp/IO@miR-21/124 application. Obtained results indicated that fabricated nanocomposite might impact proper regeneration of osteoporotic bone, restoring the balance between osteoblasts and osteoclast.

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Section: Discussionmentioning

confidence: 99%

Nanohydroxyapatite (nHAp) Doped with Iron Oxide Nanoparticles (IO), miR-21 and miR-124 Under Magnetic Field Conditions Modulates Osteoblast Viability, Reduces Inflammation and Inhibits the Growth of Osteoclast – A Novel Concept for Osteoporosis Treatment: Part 1

Marycz

Śmieszek

Marcinkowska

et al. 2021

IJN

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“…Besides that, the nanoparticles of magnetic iron oxide show excellent biodegradable, biocompatible, and nontoxic properties (Zhang et al, 2021). Several studies have looked at the insertion of some iron oxide‐based into the HAP, such as HAP‐Fe (Pon‐on et al, 2007), Ca 9.4 Fe 0.4 Mn 0.2 (PO 4 ) 6 (OH) 2 (Pon‐on et al, 2008), Fe 3 O 4 ‐HAP (Kuda et al, 2009; Tkachenko & Kamzin, 2016; Vlasova et al, 2017), Fe 3 O 4 /HAP nanoparticles (Liu et al, 2012), HAP‐BaFe 12 O 19 (Jarupoom & Jaita, 2015b). As the above results clearly illustrate, the iron oxide‐based compounds were the effective additive for improving both magnetic and higher apatite forming ability.…”

Section: Introductionmentioning

confidence: 99%

Enhanced magnetic performance and in‐vitro apatite‐forming ability of the CoFe₂O₄ doped nano‐hydroxyapatite porous bioceramics

Jaita

Jarupoom

2023

Microscopy Res & Technique

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In this research, the bioceramics system of nano-hydroxyapatite-cobalt ferrite or Ca 10 (PO4) 6 (OH) 2 /xCoFe 2 O 4 (HAP/xCF), where x = 0-3 vol%, were studied. The effect of CF concentration on phase evolution, physical, microstructure, mechanical, and magnetic properties as well as the in-vitro apatite-forming ability and cell culture analysis of the HAP ceramic was investigated. XRD revealed that all HAP/xCF ceramics showed high purity of hydroxyapatite with calcium and phosphate. However, the peak of the CF phase is noted for the HAP + 3 vol% CF ceramic. The densification and mechanical properties (HV, HK, σ c , and σ f ) decreased with increasing the CF additive, which correlated to all HAP/xCF ceramics exhibited porous structure with increasing the percentage of porosity. The average grain size also increased with increasing the CF content. An improvement of magnetic behavior, which increasing of the M r , H c , and μ B values, was obtained for the higher CF ceramics. In-vitro apatite-forming ability test suggested that the HAP + 3 vol% CF porous ceramic has a good apatite-forming ability. The cell culture analysis indicated that the proliferation of cells was above 97% for the HAP + 3 vol% CF porous ceramic, which means that the prepared ceramic is biocompatible. Based on the obtained results indicated that these ceramics are promising biomedical application candidates. Research HighlightsWe fabricated the HAP/xCF ceramics by a simple solid-state reaction method. The addition of CF into HAP exhibited magnetic improvement and produced the porous ceramic, which caused good apatite-forming ability. The cell culture analysis indicated that the HAP + 3 vol% CF ceramic is biocompatible.

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“…Mechanosynthesis is a relatively new method for synthesizing calcium phosphate. It is suitable to prepare nanopowders [17]- [19]. High-energy ball milling presents many advantages for industrial applications where simplicity and high productivity are of paramount importance.…”

Section: Introductionmentioning

confidence: 99%

Mechanosynthesis of carbonate and lithium co-substituted hydroxyfluorapatite

Hajji

Gallet

Saviot

et al. 2022

Materials Research Bulletin

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Mechanosynthesis of hydroxyapatite–ferrite composite nanopowder

Cited by 8 publications

References 40 publications

Nanohydroxyapatite (nHAp) Doped with Iron Oxide Nanoparticles (IO), miR-21 and miR-124 Under Magnetic Field Conditions Modulates Osteoblast Viability, Reduces Inflammation and Inhibits the Growth of Osteoclast – A Novel Concept for Osteoporosis Treatment: Part 1

Nanohydroxyapatite (nHAp) Doped with Iron Oxide Nanoparticles (IO), miR-21 and miR-124 Under Magnetic Field Conditions Modulates Osteoblast Viability, Reduces Inflammation and Inhibits the Growth of Osteoclast – A Novel Concept for Osteoporosis Treatment: Part 1

Enhanced magnetic performance and in‐vitro apatite‐forming ability of the CoFe₂O₄ doped nano‐hydroxyapatite porous bioceramics

Mechanosynthesis of carbonate and lithium co-substituted hydroxyfluorapatite

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Mechanosynthesis of hydroxyapatite–ferrite composite nanopowder

Cited by 8 publications

References 40 publications

Nanohydroxyapatite (nHAp) Doped with Iron Oxide Nanoparticles (IO), miR-21 and miR-124 Under Magnetic Field Conditions Modulates Osteoblast Viability, Reduces Inflammation and Inhibits the Growth of Osteoclast – A Novel Concept for Osteoporosis Treatment: Part 1

Nanohydroxyapatite (nHAp) Doped with Iron Oxide Nanoparticles (IO), miR-21 and miR-124 Under Magnetic Field Conditions Modulates Osteoblast Viability, Reduces Inflammation and Inhibits the Growth of Osteoclast – A Novel Concept for Osteoporosis Treatment: Part 1

Enhanced magnetic performance and in‐vitro apatite‐forming ability of the CoFe2O4 doped nano‐hydroxyapatite porous bioceramics

Mechanosynthesis of carbonate and lithium co-substituted hydroxyfluorapatite

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Enhanced magnetic performance and in‐vitro apatite‐forming ability of the CoFe₂O₄ doped nano‐hydroxyapatite porous bioceramics