Mechanosensitive physiology of chlamydomonas reinhardtii under direct membrane distortion

Min, Seul Ki; Yoon, Gwang Heum; Joo, Jung Hyun; Sim, Sang Jun; Shin, Hwa Sung

doi:10.1038/srep04675

Cited by 22 publications

(22 citation statements)

References 40 publications

(48 reference statements)

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“…We propose that, parallel to normal stem cell biology where progenitor cells regulate adult stem cell homeostasis (9), BMP2 produced by progenitor cells acts as a feedback mechanism promoting CSC expansion. Such a mechanism would assure maintenance of a stable CSC percentage as the progenitor pool expands and would be consistent with other reports specifically indicating a role for BMP2 in stem cell maintenance (24).…”

Section: Cd133supporting

confidence: 73%

Identifying an ovarian cancer cell hierarchy regulated by bone morphogenetic protein 2

Choi

Ingram

Yang

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Whether human cancer follows a hierarchical or stochastic model of differentiation is controversial. Furthermore, the factors that regulate cancer stem-like cell (CSC) differentiation potential are largely unknown. We used a novel microfluidic single-cell culture method to directly observe the differentiation capacity of four heterogeneous ovarian cancer cell populations defined by the expression of the CSC markers aldehyde dehydrogenase (ALDH) and CD133. We evaluated 3,692 progeny from 2,833 cells. CD133+ cell expansion while suppressing the proliferation of ALDH − CD133 − cells. As such, BMP2 suppressed bulk cancer cell growth in vitro but increased tumor initiation rates, tumor growth, and chemotherapy resistance in vivo whereas BMP2 knockdown reduced CSC numbers, in vivo growth, and chemoresistance. These data suggest a hierarchical differentiation pattern in which BMP2 acts as a feedback mechanism promoting ovarian CSC expansion and suppressing progenitor proliferation. These results explain why BMP2 suppresses growth in vitro and promotes growth in vivo. Together, our results support BMP2 as a therapeutic target in ovarian cancer.ovarian cancer | cancer stem cells | BMP2 | differentiation capacity | hierarchical

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Section: Cd133supporting

confidence: 73%

Identifying an ovarian cancer cell hierarchy regulated by bone morphogenetic protein 2

Choi

Ingram

Yang

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…From a technological perspective, we anticipate that these insights may inspire new design principles for the guidance of cellular motion, e.g. for the targeted delivery of (pharmacological) cargo [36] and for directing motile eukaryotic cells through microfluidic channels in bioengineering applications [37][38][39], complementary to existing rectification approaches [29]. Moreover, an immediate consequence of enhanced detention times at a highly curved wall is a greater likelihood for the adhesion of planktonic cells at walls, which can trigger the colonization and formation of biofilms in liquid-immersed porous media.…”

Section: Discussionmentioning

confidence: 99%

Curvature-Guided Motility of Microalgae in Geometric Confinement

Ostapenko¹,

Schwarzendahl²,

Böddeker³

et al. 2018

Phys. Rev. Lett.

110

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Microorganisms, such as bacteria and microalgae, often live in habitats consisting of a liquid phase and a plethora of interfaces. The precise ways in which these motile microbes behave in their confined environment remain unclear. Using experiments, Brownian dynamics simulations, and analytical theory, we study the motility of a single Chlamydomonas microalga in an isolated microhabitat with controlled geometric properties. We demonstrate how the geometry of the habitat controls the cell's navigation in confinement. The probability of finding the cell swimming near the boundary scales linearly with the wall curvature, as seen for both circular and elliptical chambers. The theory, utilizing an asymmetric dumbbell model of the cell and steric wall interactions, captures this curvature-guided navigation quantitatively with no free parameters.

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“…Mechanical stress in microalgae is transmitted through the mechanosensitive ion channel of calcium ion19. As seen in Fig.…”

Section: Discussionmentioning

confidence: 99%

Nanocellulose size regulates microalgal flocculation and lipid metabolism

Min

Shin

2016

Sci Rep

Self Cite

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Harvesting of microalgae is a cost-consuming step for biodiesel production. Cellulose has recently been studied as a biocompatible and inexpensive flocculant for harvesting microalgae via surface modifications such as cation-modifications. In this study, we demonstrated that cellulose nanofibrils (CNF) played a role as a microalgal flocculant via its network geometry without cation modification. Sulfur acid-treated tunicate CNF flocculated microalgae, but cellulose nanocrystals (CNC) did not. In addition, desulfurization did not significantly influence the flocculation efficiency of CNF. This mechanism is likely related to encapsulation of microalgae by nanofibrous structure formation, which is derived from nanofibrils entanglement and intra-hydrogen bonding. Moreover, flocculated microalgae were subject to mechanical stress resulting in changes in metabolism induced by calcium ion influx, leading to upregulated lipid synthesis. CNF do not require surface modifications such as cation modified CNC and flocculation is derived from network geometry related to nanocellulose size; accordingly, CNF is one of the least expensive cellulose-based flocculants ever identified. If this flocculant is applied to the biodiesel process, it could decrease the cost of harvest, which is one of the most expensive steps, while increasing lipid production.

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Mechanosensitive physiology of chlamydomonas reinhardtii under direct membrane distortion

Cited by 22 publications

References 40 publications

Identifying an ovarian cancer cell hierarchy regulated by bone morphogenetic protein 2

Identifying an ovarian cancer cell hierarchy regulated by bone morphogenetic protein 2

Curvature-Guided Motility of Microalgae in Geometric Confinement

Nanocellulose size regulates microalgal flocculation and lipid metabolism

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