Mechanosensitive ion channels in nonspecialized cells

Sachs, Frederick; Morris, Catherine E.

doi:10.1007/bfb0004985

Cited by 237 publications

(208 citation statements)

References 216 publications

(289 reference statements)

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“…[61, 124,136,144,165,176,178]. Because of its trivalency, Gd 3+ will bind with high affinity to negative groups on proteins, lipids, and polysaccharides, as well as any inorganic anions present in solution [17,61].…”

Section: "Mechanopharmaceutics"mentioning

confidence: 99%

“…Although the distinction between adaptation and inactivation still needs to be made, the observation is significant because the clear absence of any CSK excludes its involvement in these transient kinetics. One possible explanation is timedependent sliding/relaxation of the two monolayers that results in relaxation of the gating tension [144]. Because it was clearly demonstrated that channels could not be reactivated without a finite time for recovery, the phenomenon was referred to as desensitization.…”

Section: Mscs and Mscl In Escherichia Colimentioning

confidence: 99%

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Twenty odd years of stretch-sensitive channels

Hamill

2006

Pflugers Arch - Eur J Physiol

110

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After formation of the giga-seal, the membrane patch can be stimulated by hydrostatic or osmotic pressure gradients applied across the patch. This feature led to the discovery of stretch-sensitive or mechanosensitive (MS) channels, which are now known to be ubiquitously expressed in cells representative of all the living kingdoms. In addition to mechanosensation, MS channels have been implicated in many basic cell functions, including regulation of cell volume, shape, and motility. The successful cloning, overexpression, and crystallization of bacterial MS channel proteins combined with patch clamp and modeling studies have provided atomic insight into the working of these nanomachines. In particular, studies of MS channels have revealed new understanding of how the lipid bilayer modulates membrane protein function. Three major membrane protein families, transient receptor potential, 2 pore domain K + , and the epithelial Na + channels, have been shown to form MS channels in animal cells, and their polymodal activation embrace fields far beyond mechanosensitivity. The discovery of new drugs highly selective for MS channels ("mechanopharmaceutics") and the demonstration of MS channel involvement in several major human diseases ("mechanochannelopathies") provide added motivation for devising new techniques and approaches for studying MS channels.

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Section: "Mechanopharmaceutics"mentioning

confidence: 99%

Section: Mscs and Mscl In Escherichia Colimentioning

confidence: 99%

Twenty odd years of stretch-sensitive channels

Hamill

2006

Pflugers Arch - Eur J Physiol

110

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“…Previously we demonstrated that stretch-induced SAC activation augments Na ϩ -K ϩ -ATPase activity (19). To relate plasma membrane tension to SAC activation, we employed a prevalent theory (29,41,49,50) that the probability, P o , of a SAC being open to ion traffic is determined by a Boltzmann probability distribution dependent on plasma membrane tension, T:…”

Section: Resultsmentioning

confidence: 99%

“…tension change required to cause an e-fold increase in relative channel activity. For our purposes, we performed parametric simulations over a range of values for , and T 1/2 corresponding to a range of parameter values reported in the SAC classification literature (24,29,30,41,49,50). Model-generated membrane tension was used to calculate a P o "wave" via Eq.…”

Section: Resultsmentioning

confidence: 99%

“…The gray arrow in Fig. 1 represents a biophysics theory, which states that SAC opening probability is described by a Boltzmann distribution dependent on tension over the SAC (22,24,29,30,32,41). The dashed black arrows represent relationships between stretch and plasma membrane tension, including a negative feedback mechanism created by tension-reducing lipid insertion, all of which we wish to predict with a mathematical model fit to data derived from the appropriate literature.…”

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confidence: 99%

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Modeling the effect of stretch and plasma membrane tension on Na⁺-K⁺-ATPase activity in alveolar epithelial cells

Fisher¹,

Margulies²

2007

American Journal of Physiology-Lung Cellular and Molecular Phys

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Fisher, Jacob L., and Susan S. Margulies. Modeling the effect of stretch and plasma membrane tension on Na ϩ -K ϩ -ATPase activity in alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol 292: L40-L53, 2007. First published August 4, 2006; doi:10.1152/ajplung.00425.2005.-While a number of whole cell mechanical models have been proposed, few, if any, have focused on the relationship among plasma membrane tension, plasma membrane unfolding, and plasma membrane expansion and relaxation via lipid insertion. The goal of this communication is to develop such a model to better understand how plasma membrane tension, which we propose stimulates Na ϩ -K ϩ -ATPase activity but possibly also causes cell injury, may be generated in alveolar epithelial cells during mechanical ventilation. Assuming basic relationships between plasma membrane unfolding and tension and lipid insertion as the result of tension, we have captured plasma membrane mechanical responses observed in alveolar epithelial cells: fast deformation during fast cyclic stretch, slower, time-dependent deformation via lipid insertion during tonic stretch, and cell recovery after release from stretch. The model estimates plasma membrane tension and predicts Na ϩ -K ϩ -ATPase activation for a specified cell deformation time course. Model parameters were fit to plasma membrane tension, whole cell capacitance, and plasma membrane area data collected from the literature for osmotically swollen and shrunken cells. Predictions of membrane tension and stretch-stimulated Na ϩ -K ϩ -ATPase activity were validated with measurements from previous studies. As a proof of concept, we demonstrate experimentally that tonic stretch and consequent plasma membrane recruitment can be exploited to condition cells against subsequent cyclic stretch and hence mitigate stretchinduced responses, including stretch-induced cell death and stretch-induced modulation of Na ϩ -K ϩ -ATPase activity. Finally, the model was exercised to evaluate plasma membrane tension and potential Na ϩ -K ϩ -ATPase stimulation for an assortment of traditional and novel ventilation techniques.ventilator-induced lung injury; lipid trafficking; edema recovery INVESTIGATORS HAVE FOUND alveolar epithelial stretch magnitude to be related to cell injury in vitro (55, 56) and tidal volume to be related to ventilator-induced lung injury (VILI) in animal models (12-15). Studies have revealed that the frequency of epithelial stretch or ventilation can also be an important factor in cell injury (56, 60). The model developed in this study examines how combinations of amplitude and frequency in ventilation procedures affect Na ϩ -K ϩ -ATPase activity via hypothesized pathways including alveolar epithelial cell plasma membrane tension and stretch-activated channel (SAC) stimulation.To date, numerous models have been proposed to describe cellular mechanical behavior, from early continuum viscoelastic representations for erythrocytes and leukocytes (11,27,65) to more sophisticated variations that modeled cell membrane an...

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Multiple triggers of oocyte maturation in nemertean worms: The roles of calcium and serotonin

Stricker

Smythe

2000

J. Exp. Zool.

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Mechanosensitive ion channels in nonspecialized cells

Cited by 237 publications

References 216 publications

Twenty odd years of stretch-sensitive channels

Twenty odd years of stretch-sensitive channels

Modeling the effect of stretch and plasma membrane tension on Na⁺-K⁺-ATPase activity in alveolar epithelial cells

Multiple triggers of oocyte maturation in nemertean worms: The roles of calcium and serotonin

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Mechanosensitive ion channels in nonspecialized cells

Cited by 237 publications

References 216 publications

Twenty odd years of stretch-sensitive channels

Twenty odd years of stretch-sensitive channels

Modeling the effect of stretch and plasma membrane tension on Na+-K+-ATPase activity in alveolar epithelial cells

Multiple triggers of oocyte maturation in nemertean worms: The roles of calcium and serotonin

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Modeling the effect of stretch and plasma membrane tension on Na⁺-K⁺-ATPase activity in alveolar epithelial cells