Mechanosensing dysregulation in the fibroblast: A hallmark of the aging heart

Angelini, Aude; Trial, JoAnn; Ortiz-Urbina, Jesús; Cieslik, Katarzyna A.

doi:10.1016/j.arr.2020.101150

Cited by 48 publications

(48 citation statements)

References 205 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several lines of evidence have indicated that α-SMA-associated contraction is involved in ECM organization 67 , 70 , 71 . The up-regulation of α-SMA is also important for myofibroblast focal adhesion, maturation and adhesive capability 72 , 73 . In tubulointerstitial renal fibrosis, TGF-β1 enhances α-SMA expression in fibroblasts to form stress fibers and focal adhesion, resulting in the fibrotic tissue contraction 74 , 75 .…”

Section: Discussionmentioning

confidence: 99%

Effects of secretome derived from macrophages exposed to calcium oxalate crystals on renal fibroblast activation

et al. 2021

View full text Add to dashboard Cite

The association between kidney stone disease and renal fibrosis has been widely explored in recent years but its underlying mechanisms remain far from complete understanding. Using label-free quantitative proteomics (nanoLC-ESI-LTQ-Orbitrap MS/MS), this study identified 23 significantly altered secreted proteins from calcium oxalate monohydrate (COM)-exposed macrophages (COM-MP) compared with control macrophages (Ctrl-MP) secretome. Functional annotation and protein-protein interactions network analysis revealed that these altered secreted proteins were involved mainly in inflammatory response and fibroblast activation. BHK-21 renal fibroblasts treated with COM-MP secretome had more spindle-shaped morphology with greater spindle index. Immunofluorescence study and gelatin zymography revealed increased levels of fibroblast activation markers (α-smooth muscle actin and F-actin) and fibrotic factors (fibronectin and matrix metalloproteinase-9 and -2) in the COM-MP secretome-treated fibroblasts. Our findings indicate that proteins secreted from macrophages exposed to COM crystals induce renal fibroblast activation and may play important roles in renal fibrogenesis in kidney stone disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of secretome derived from macrophages exposed to calcium oxalate crystals on renal fibroblast activation

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Potentially in combination with other technologies, allowing super‐resolution imaging in tissues, [38] it should enable the molecular scale analysis of talin in numerous physiological and pathophysiological conditions such as cancer, [39] fibrosis [40] and aging. [41] The ability of PIPKIγ to detect the mouse and the human talin protein will be especially useful in this context, as it should also enable the investigation of tissue samples and biopsies from patients.…”

Section: Discussionmentioning

confidence: 99%

Peptide‐PAINT Enables Investigation of Endogenous Talin with Molecular Scale Resolution in Cells and Tissues

2021

View full text Add to dashboard Cite

Talin is a cell adhesion molecule that is indispensable for the development and function of multicellular organisms. Despite its central role for many cell biological processes, suitable methods to investigate the nanoscale organization of talin in its native environment are missing. Here, we overcome this limitation by combining single-molecule resolved PAINT (points accumulation in nanoscale topography) imaging with the IRIS (image reconstruction by integrating exchangeable singlemolecule localization) approach, enabling the quantitative analysis of genetically unmodified talin molecules in cells. We demonstrate that a previously reported peptide can be utilized to specifically label the two major talin isoforms expressed in mammalian tissues with a localization precision of < 10 nm. Our experiments show that the methodology performs equally well as state-of-the-art single-molecule localization techniques, and the first applications reveal a thus far undescribed cell adhesion structure in differentiating stem cells. Furthermore, we demonstrate the applicability of this peptide-PAINT technique to mouse tissues paving the way to single-protein imaging of endogenous talin proteins under physiologically relevant conditions.

show abstract

“… 45 Simultaneously, α-SMA, expressed by myofibroblasts, can also play as the specific marker of myofibroblast proliferation. 46 When the acute injury gradually becomes chronic, inflammatory cells continue to infiltrate and secrete inflammatory cytokines, resulting in abnormally increased TGF-β. Thus, a large number of myofibroblasts will proliferate in damaged muscle tissue producing excessive ECM, including collagen and FN.…”

Section: Discussionmentioning

confidence: 99%

Pressing Intervention Promotes the Skeletal Muscle Repair of Traumatic Myofascial Trigger Points in Rats

Jiang¹,

Xiang²,

Liú³

et al. 2021

JPR

View full text Add to dashboard Cite

Objective: To observe the effect of pressing intervention on the skeletal muscle repair of myofascial trigger points (MTrPs) in rats and explore the mechanism of pressing intervention on the deactivation of trigger points. Methods: Thirty SPF rats were randomly divided into blank group, model group and press group, with 10 rats in each group. The MTrPs models were established by blunt striking plus eccentric exercise, and then evaluated. The press group was given a pressing intervention with a self-made device for 14 days, and the rats in the other two groups were fed normally. Soft tissue tension (STT) D 0.2 and pressure pain threshold (PPT) were measured before and after intervention. The skeletal muscle tissue at MTrPs was extracted and assessed by hematoxylin-eosin (HE) and Masson staining. The expression of collagen I, collagen III, α-smooth muscle actin (α-SMA), myosin heavy chain (MHC) and fibronectin (FN) were detected by Western Blotting. Enzyme linked immunosorbent assay (ELISA) was used to evaluate the expression of substance P (SP), 5-hydroxytryptamine (5-HT), cyclooxygenase 2 (COX-2) and prostaglandin E2 (PGE2). Results: (1) Compared with the blank group, the PPT and D 0.2 reduced (P < 0.05) in the model group; while compared with the model group, the PPT and D 0.2 increased (P < 0.05) in the press group. (2) Compared with the blank group, the model group showed obvious spontaneous potentials with higher amplitude and frequency, which were also much higher than those of the press group (P < 0.05). (3) The HE and Masson staining results showed evident fibrosis in the muscle tissue of the model group, with a larger area of collagen fibers relative to that of the press group (P < 0.05). ( 4) The amount of collagen I, collagen III, FN, α-SMA, SP, 5-HT, COX-2 and PGE2 increased and the content of MHC decreased (P < 0.05) in the model group, as compared to the blank group; while all the substances (P < 0.05), instead of MHC which increased (P < 0.05), in the press group were decreased as compared to the model group. Conclusion:Pressing intervention on the MTrPs in rats can alleviate chronic inflammation, inhibit fibrosis, promote skeletal muscle repair and relieve pain.

show abstract

Mechanosensing dysregulation in the fibroblast: A hallmark of the aging heart

Cited by 48 publications

References 205 publications

Effects of secretome derived from macrophages exposed to calcium oxalate crystals on renal fibroblast activation

Effects of secretome derived from macrophages exposed to calcium oxalate crystals on renal fibroblast activation

Peptide‐PAINT Enables Investigation of Endogenous Talin with Molecular Scale Resolution in Cells and Tissues

Pressing Intervention Promotes the Skeletal Muscle Repair of Traumatic Myofascial Trigger Points in Rats

Contact Info

Product

Resources

About