Mechanoregulation of angiopoietin-like 4 in epithelial-mesenchymal transition and cancer metastasis

Abstract

The contributions of the co-authors are as follows:• I performed the RNA-seq and microarray data mining, quality control of RNA-seq data, processing of raw RNA-seq data into unified FeatureCounts files for bioinformatics analysis, analysis of RNA-seq data, generation of new RNA-seq data (3D cultured MKN74 cells), characterization of cancer cell spheroids, characterization of 3D collagen-alginate and PEGDA-GelMA cultures, cell imaging, most of the qPCR works, most of the dose response studies, identification of DNAse I hypersensitive regions in human Angptl4 gene, qChIP and animal studies.• H.S. Cheng wrote the codes for RNA-seq data analysis and provided mentorship to me whenever necessary.• J.J.H. Lim performed the experiments on bladder cancer cells and mined the cancer clinical data from databases as part of his undergraduate final year project.• J.X.T. Lee generated the RNA-seq data for 2D cultured MKN74 cells.• M.I.G. Vos and Y.S. Yip provided support to me for animal studies and imaging.• J.J.H. Lim and D. Chua performed the immunoblots.• D. Chua also performed the re-ChIP and zymography.• C.B. Too performed the ASO knockdown and assisted in the imaging of MKN74 spheroids.