Mechanoreceptors in the Palmar Wrist Ligaments

Petrie, S.; Collins, J.; Solomonow, M.; Wink, Carole S.; Chuinard, R.

doi:10.1302/0301-620x.79b3.0790494

Cited by 17 publications

(7 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Examinations of serial slices are also important for adequately identifying mechanoreceptors, due to their relative large size and their resemblance to blood vessels in partial slices of ligament sectioning [17]. Few previous studies [7, 18, 22, 23, 28] have utilized serial sections of whole ligaments. In fact, only Shimizu et al [28], Wada et al [29], and Krauspe et al [18] have performed studies using serially sectioned whole ligaments.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical study of mechanoreceptors in the tibial remnant of the ruptured anterior cruciate ligament in human knees

Lee

Min

Choi

et al. 2009

Knee Surg Sports Traumatol Arthrosc

View full text Add to dashboard Cite

This study was performed to identify the mechanoreceptors in the tibial remnants of ruptured human anterior cruciate ligaments (ACL) by immunohistochemical staining. Thirty-six specimens of tibial ACL remnants were obtained from patients with ACL ruptures during arthroscopic ACL reconstruction. As control, two normal ACL specimens were taken from healthy knee amputated at thigh level due to trauma. The specimen was serially sectioned at 40 mum. In control group, the average number of sections per specimen was 132, and a total of 264 slices were available. In remnant group, the average number of sections per specimen was 90, and a total of 3,251 slices were available. Immunohistochemical staining was performed to detect the neural element of mechanoreceptors. Histologic examinations were performed under a light microscope and interpreted by a pathologist. Nineteen (8 Ruffini, 11 Golgi) mechanoreceptors were identified in the two normal ACLs, which were evenly distributed at both tibial and femoral attachments. In the remnant group, mechanoreceptors were observed in 12 out of 36 cases (33%), and a total of 17 (6 Ruffini and 11 Golgi) mechanoreceptors observed. No significant differences in the harvest volume, number of sections, age, or time between injury to surgery was observed between the 12 mechanoreceptor-present and the 24 mechanoreceptor-absent ones. The presence of mechanoreceptor at the tibial remnants of torn ACLs was verified. The immunohistochemical staining methodology proved useful, but requires further refinement. Although the mechanoreceptors were detected relatively less frequently than expected, the authors consider that it does not negate the necessity of remnant-preserving ACL reconstruction.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunohistochemical study of mechanoreceptors in the tibial remnant of the ruptured anterior cruciate ligament in human knees

Lee

Min

Choi

et al. 2009

Knee Surg Sports Traumatol Arthrosc

View full text Add to dashboard Cite

show abstract

“…In an attempt to provide a reasonable explanation supporting this unexpected result, the relationship between peripheral and central factors in voluntary activation pattern has to be taken into account. Maximal voluntary force is governed by the activation of the alpha-motoneurons originated by the central nervous system, but central neural drive is continuously adjusted in accordance with the peripheral information coming from mechanoreceptors in the ligaments 12,26,27,44 and other afferents. 19 A decrease in MVC and quadriceps EMG activity was observed after 20-minute vibrations were applied to the infrapatellar tendon of the uninjured side in ACL patients.…”

Section: Discussionmentioning

confidence: 99%

Neuromuscular Response to Cyclic Loading of the Anterior Cruciate Ligament

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Of these, the gold chloride method has been frequently employed [Freeman and Wyke, 1967;Petrie et al, 1997], but one disadvantage is that it stains elastic fibers in blood vessels in addition to nerve tissue in ligaments, thus providing nonspecific imaging of neural elements in tissue. Antibody immunohistochemical analysis is the most precise of these techniques, as it stains only designated markers.…”

Section: Introductionmentioning

confidence: 99%

Immunofluorescent Triple-Staining Technique to Identify Sensory Nerve Endings in Human Thumb Ligaments

Lee

Ladd

Hagert

2011

Cells Tissues Organs

View full text Add to dashboard Cite

Ligament innervation purportedly plays a critical role in stability, proprioception and pathology of joints with minimal bony constraints. The human thumb carpometacarpal (CMC) joint is such a joint: with a complex saddle configuration and wide circumduction, its constraint is primarily ligamentous and it is prone to osteoarthritis. CMC reconstruction is the most commonly performed arthritis surgery in the upper extremity. Little, however, is known about CMC ligament innervation. We describe a novel triple-staining immunofluorescence technique using the markers for low-affinity neurotrophin receptor p75, the pan-neuronal marker protein gene product (PGP) 9.5 and 4′,6′-diamidino-2-phenylindole (DAPI) to simultaneously detect and differentiate between specific sensory nerve endings: the Pacini corpuscles, the Ruffini endings and nerve fascicles. Five primary CMC ligaments (dorsal radial, dorsal central, posterior oblique, anterior oblique and ulnar collateral ligaments) were harvested from 10 fresh-frozen human cadaver hands. Following paraffin sectioning, each ligament was stained using a triple-stain technique and imaged with fluorescence microscopy. Multidimensional acquisition permitted simultaneous capture of images at different wavelengths. Pacini corpuscles were distinguished by their distinct p75 immunoreactive capsules, and Ruffini endings by their overlapping p75 and PGP9.5 immunoreactive dendritic nerve endings. Simultaneous use of PGP9.5, p75 and DAPI immunofluorescence to analyze innervation patterns in human ligaments provides descriptive analysis of staining patterns and receptor structure as well as clues as to the proprioceptive function of CMC ligaments and the joint as a whole. Our novel findings of CMC ligament innervation augment the study of normal and pathological joint mechanics in this joint so prone to osteoarthritis.

show abstract

Mechanoreceptors in the Palmar Wrist Ligaments

Cited by 17 publications

References 11 publications

Immunohistochemical study of mechanoreceptors in the tibial remnant of the ruptured anterior cruciate ligament in human knees

Immunohistochemical study of mechanoreceptors in the tibial remnant of the ruptured anterior cruciate ligament in human knees

Neuromuscular Response to Cyclic Loading of the Anterior Cruciate Ligament

Immunofluorescent Triple-Staining Technique to Identify Sensory Nerve Endings in Human Thumb Ligaments

Contact Info

Product

Resources

About