MECHANOLUMINESCENCE OF WALKER-256 CARCINOSARCOMA CELLS INDUCED BY MAGNETO-MECHANOCHEMICAL EFFECTS OF Fe₃O₄–Au NANOCOMPOSITE

Abstract

Magnetic fields have been used to deliver magnetic nanocomposites (MNCs) and alter mechanochemical transduction pathways in malignant tumors. We study mechanoluminescence (ML) of Walker-256 carcinosarcoma cells induced by the magneto-mechanochemical effects of Fe3O4–Au MNCs under a nonuniform rotating magnetic field (RMF). Photoluminescence (PL) and Raman spectra were recorded to investigate the optical response of MNCs. The PL spectrum of MNCs showed three broad emission bands with peaks at 525, 570 and 680 nm. MNCs underwent a phase transition attributed to localized surface plasmon resonance as indicated by the Raman spectra. ML intensity recorded from MNCs[Formula: see text][Formula: see text][Formula: see text]cells[Formula: see text][Formula: see text][Formula: see text]RMF was 3.5 and 1.4 times greater than chemiluminescence (CL) of MNCs[Formula: see text][Formula: see text][Formula: see text]cells and cells alone, respectively ([Formula: see text] 0.05). ML exhibited lesser variation than CL. The nonuniform distribution of a magnetic force exerted on MNCs resulted in more symmetric distributions of ML signals. Therefore, the observed ML emission could originate from the magneto-mechanochemically and light-induced free radical reactions in cancer cells in response to MNCs[Formula: see text][Formula: see text][Formula: see text]RMF. The magneto-mechanochemical effects have the possibility to translate ML to cancer diagnosis and treatment by providing additional information about changes in breaking asymmetry to symmetric processes at the quantum level.

Characterization and antitumor effect of doxorubicin-loaded Fe3O4–Au nanocomposite synthesized by electron beam evaporation for magnetic nanotheranostics

“…6 Furthermore, the optical properties of gold can be used to promote plasmon-assisted free radical production by MNC under the influence of magnetic fields. 7,8…”

“…6 Furthermore, the optical properties of gold can be used to promote plasmonassisted free radical production by MNC under the inuence of magnetic elds. 7,8 It is important to begin with an understanding that mitochondria are one of the major sources of free radicals in mammalian cells due to proton-coupled electron transfer processes. 9 ROS generation is a widely accepted mechanism by which many anticancer drugs, such as doxorubicin (DOX), act on the tumor and its microenvironment.…”