Mechanoenzymatic reactions for the hydrolysis of PET

Ambrose-Dempster, Esther; Leipold, Leona; Dobrijevic, Dragana; Bawn, Maria; Carter, Eve M.; Stojanovski, Gorjan; Sheppard, Tom D.; Jeffries, Jack W. E.; Ward, John M.; Hailes, Helen C.

doi:10.1039/d3ra01708g

Cited by 7 publications

(3 citation statements)

References 60 publications

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“…The mechanochemical hydrolysis of PET has been reported previously. 35–38 The examples used activation additives such as enzymes and sodium hydroxide, transferring similar conventional combinations to mechanochemical conditions. Thus, additional purification, neutralization, and waste generation are inevitable.…”

Section: Resultsmentioning

confidence: 99%

Chemical recycling of polycarbonate and polyester without solvent and catalyst: mechanochemical methanolysis

Lee,

Yoo,

Borchardt

et al. 2024

Green Chem.

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Section: Resultsmentioning

confidence: 99%

Chemical recycling of polycarbonate and polyester without solvent and catalyst: mechanochemical methanolysis

Lee,

Yoo,

Borchardt

et al. 2024

Green Chem.

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“…(2023) reported PET degradation using a combination of PETase powder and lyophilised whole cell PETase in ball‐milling with 1.9 μL/nmol KPi buffer. This reactive aging process using Is ‐PETase led to 30 times less solvent usage compared to industrial PET breakdown systems and 2600 fold reduction compared to PETase hydrolysis in‐solution [26] …”

Section: Ball‐millingmentioning

confidence: 99%

Mechanoenzymatic Reactions – Challenges and Perspectives

Hollenbach,

Ochsenreither

2023

ChemCatChem

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Mechanoenzymology has emerged as a recent topic in modern research and process design in the transition towards green chemistry. Due to the almost solvent‐free character of mechanoenzymatic reactions solvent usage is considerably reduced and waste can be avoided. Moreover, mechanoenzymatic reactions show highly promising space‐time yields and conversions, however, are still less investigated than their chemical counterparts. The selectivity of enzymes is an important feature for designing green processes and avoiding formation of by‐products. In this review, different mechanoenzymatic strategies are pointed out, involving the most common applied devices, i.e., shaker ball mills, planetary ball mills and twin‐screw extruders. Their compatibility with upscaling and continuous processes is discussed and reusability of enzymes in the different mechanoenzymatic processes is evaluated. In addition, learnings from mechanochemistry are presented and their potential benefits for mechanoenzymology are outlined.

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“…On the other hand, recently the concept of circular carbon economy based on biotechnological plastic recycling has become a thriving research area in recent years ( Ru, Huo, and Yang, 2020 ; Ellis et al, 2021a ; Kakadellis and Rosetto, 2021 ; Simon et al, 2021 ). In addition to the traditional mechanical recycling and chemical recycling ( Mederake, 2022 ), biocatalytic depolymerization catalyzed by PET-degrading enzymes (e.g., PETase) has emerged as a promising and sustainable alternative for PET upcycling and enable circularity ( Magalhães, Cunha, and Sousa, 2021 ; Cao et al, 2022 ; Lai et al, 2022 ; Ambrose-Dempster et al, 2023 ). A variety of naturally occurring plastic degrading enzymes have been discovered and characterized from microbial sources ( Kawai, 2021 ; Erickson et al, 2022 ; Khairul Anuar et al, 2022 ; Wei et al, 2022 ; Zhu, Wang, and Wei, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Current advances in the structural biology and molecular engineering of PETase

Liu,

Wang,

Yang

et al. 2023

Front. Bioeng. Biotechnol.

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Poly(ethylene terephthalate) (PET) is a highly useful synthetic polyester plastic that is widely used in daily life. However, the increase in postconsumer PET as plastic waste that is recalcitrant to biodegradation in landfills and the natural environment has raised worldwide concern. Currently, traditional PET recycling processes with thermomechanical or chemical methods also result in the deterioration of the mechanical properties of PET. Therefore, it is urgent to develop more efficient and green strategies to address this problem. Recently, a novel mesophilic PET-degrading enzyme (IsPETase) from Ideonella sakaiensis was found to streamline PET biodegradation at 30°C, albeit with a lower PET-degrading activity than chitinase or chitinase-like PET-degrading enzymes. Consequently, the molecular engineering of more efficient PETases is still required for further industrial applications. This review details current knowledge on IsPETase, MHETase, and IsPETase-like hydrolases, including the structures, ligand‒protein interactions, and rational protein engineering for improved PET-degrading performance. In particular, applications of the engineered catalysts are highlighted, including metabolic engineering of the cell factories, enzyme immobilization or cell surface display. The information is expected to provide novel insights for the biodegradation of complex polymers.

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Mechanoenzymatic reactions for the hydrolysis of PET

Abstract: Mechanoenzymatic reactions are described for the degradation of different PET materials using whole cell PETases.

Cited by 7 publications

References 60 publications

Chemical recycling of polycarbonate and polyester without solvent and catalyst: mechanochemical methanolysis

Chemical recycling of polycarbonate and polyester without solvent and catalyst: mechanochemical methanolysis

Mechanoenzymatic Reactions – Challenges and Perspectives

Current advances in the structural biology and molecular engineering of PETase

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