Mechanochemistry of an Interlocked Poly[2]catenane: From Single Molecule to Bulk Gel

Xing, Hao; Li, Zhandong; Wang, Wenbo; Liu, Peiren; Liu, Junkai; Song, Yu; Wu, Zi Liang; Zhang, Wenke; Huang, Feihe

doi:10.31635/ccschem.019.201900043

Cited by 59 publications

(38 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to classic ensemble measurements, we used atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS) to measure the strength of binding between the three different RBDs and ACE2 on a living cell directly ( Alsteens et al, 2017a ; Hinterdorfer and Dufrêne, 2006 ). AFM-SMFS is a powerful single-molecule nanotechnology that can be used like optical and magnetic tweezers to manipulate a single molecule or several molecules mechanically ( Jobst et al, 2015 ; Walder et al, 2017 ; Alonso-Caballero et al, 2021 ; Löf et al, 2019 ; Huang et al, 2019 ; Zhang et al, 2019 ; Xing et al, 2020 ). It has been widely used to study protein mechanics and protein–protein interactions, including the interaction between the spike proteins of viruses and living cells ( Kim et al, 2010 ; Cuellar-Camacho et al, 2020 ; Yang et al, 2020 ; Sieben et al, 2012 ; Alsteens et al, 2017b ; Yu et al, 2019 ; Ma et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

N501Y mutation of spike protein in SARS-CoV-2 strengthens its binding to receptor ACE2

Tian

Tong

Sun

et al. 2021

eLife

294

220

View full text Add to dashboard Cite

SARS-CoV-2 has been spreading around the world for the past year. Recently, several variants such as B.1.1.7 (alpha), B.1.351 (beta), and P.1 (gamma), which share a key mutation N501Y on the receptor-binding domain (RBD), appear to be more infectious to humans. To understand the underlying mechanism, we used a cell surface-binding assay, a kinetics study, a single-molecule technique, and a computational method to investigate the interaction between these RBD (mutations) and ACE2. Remarkably, RBD with the N501Y mutation exhibited a considerably stronger interaction, with a faster association rate and a slower dissociation rate. Atomic force microscopy (AFM)-based single-molecule force microscopy (SMFS) consistently quantified the interaction strength of RBD with the mutation as having increased binding probability and requiring increased unbinding force. Molecular dynamics simulations of RBD–ACE2 complexes indicated that the N501Y mutation introduced additional π-π and π-cation interactions that could explain the changes observed by force microscopy. Taken together, these results suggest that the reinforced RBD–ACE2 interaction that results from the N501Y mutation in the RBD should play an essential role in the higher rate of transmission of SARS-CoV-2 variants, and that future mutations in the RBD of the virus should be under surveillance.

show abstract

Section: Resultsmentioning

confidence: 99%

N501Y mutation of spike protein in SARS-CoV-2 strengthens its binding to receptor ACE2

Tian

Tong

Sun

et al. 2021

eLife

294

220

View full text Add to dashboard Cite

show abstract

“…The theoretical calculation also confirmed that there were evident non-covalent interactions between the benzodithiazole moiety and the adjacent phenyl ring (Figure S12). 52,53 Since temperature change may influence the molecular motion 54,55 and thus change the molecular conformation as well as the microenvironment of the hydrogen atoms, variable-temperature 1 H NMR experiment was thus implemented to monitor the intramolecular hydrogen bonding change of TPABSM with temperature. As shown in Figure S13, with the temperature increasing, the chemical shift of the H atom that participates in the hydrogen bonding formation (Ha) slightly shift to the higher magnetic field.…”

Section: Resultsmentioning

confidence: 99%

A Bright Deep-Red AIEgen with Locked Noncoplanar Conformation for Lipid Droplet-Specific Imaging

Xing¹,

Park²,

Zhang³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

In the field of fluorescent materials, bright deep-red/near-infrared (DR/NIR) luminogens are always in demand whose rationally and accurately design yet are challenging. Current design strategy of DR/NIR emitters mainly depends on the construction of large p-conjugation and strong donor-acceptor system, which commonly suffers the quenching factors from p-p stacking and twisted intramolecular charge transfer (TICT) effect. In this work, we employed the non-covalent interactions and constructed a luminogen with rigid and twisted conformation, which not only red-shifts the emission wavelength of the luminogen to DR/NIR region but also effectively suppresses the non-radiative decay by p-p stacking and TICT. Moreover, the rational molecular design endows the new luminogen with two-photo absorption capability and superior selectivity towards lipid droplets in different levels including living cells, tissues, and live fish embryo. This work thus presents an elegant, facile and efficient strategy for generating functional DR/NIR emitters.

show abstract

“…[ 26–39 ] Synthetic oligomers and polymers have also been investigated by SMFS, which has helped us in the understanding of their nanomechanical properties. [ 40–43 ] Many researches have been done to study the nanomechanical properties of type II helices. [ 44–46 ] However, due to the limitation of detection method and dynamic nature of the structure, it is quite challenging to investigate nanomechanical properties of individual type III supramolecular helices.…”

Section: Methodsmentioning

confidence: 99%

Nanomechanical Properties of a Supramolecular Helix Stabilized by Non‐Covalent Interactions

Wang

Shen

Song

et al. 2020

Macromol. Rapid Commun.

Self Cite

View full text Add to dashboard Cite

can be classified into three types, that is, pure covalent (type I), [5-6] covalent plus supramolecular (type II), [1-4,10-12] and pure supramolecular helices (type III). [13-16] Type I helical structure is formed mainly by covalent bond (e.g., the helical carbon nanotube). Type II helix is normally formed by a biological or synthetic macromolecule, in which backbone atoms are connected by covalent bonds, and the helical structure is stabilized by both the covalent backbone and intra-or/and intermolecular supramolecular interactions between the side groups, such as dsDNA, [1,2] polysaccharide, [3,4] and some synthetic helical polymers. [10-12] Type III helix is generally formed by supramolecular assembly, whose structure is stabilized by non-covalent supramolecular interactions. [13-16] These helical structures

show abstract

Mechanochemistry of an Interlocked Poly[2]catenane: From Single Molecule to Bulk Gel

Abstract: Citation denotes calendar and volume year of first online publication.

Cited by 59 publications

References 8 publications

N501Y mutation of spike protein in SARS-CoV-2 strengthens its binding to receptor ACE2

N501Y mutation of spike protein in SARS-CoV-2 strengthens its binding to receptor ACE2

A Bright Deep-Red AIEgen with Locked Noncoplanar Conformation for Lipid Droplet-Specific Imaging

Nanomechanical Properties of a Supramolecular Helix Stabilized by Non‐Covalent Interactions

Contact Info

Product

Resources

About