Mechanistic study of antibacterial activity of biologically synthesized silver nanocolloids

Kumar, Santosh; Singh, Mukesh; Halder, Dipankar; Mitra, Atanu

doi:10.1016/j.colsurfa.2014.02.027

Cited by 51 publications

(31 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…MIC values of the nanocolloid for E. coli and B. subtilis are 14.5 and 19.4 lg/ml, respectively. However, the MIC value of AgNPs against E. coli and B. subtilis has been observed in the range of 6-180 lg/ ml in different studies (Kumar et al 2014;Ruparelia et al 2008;Krishnaraj et al 2010;Puiso et al 2014;Chitra and Annadurai 2013). The MIC value depends on various factors including size and shape of the nanoparticles, initial concentration of the bacterial colonies and nature of the bacterial strain (Ruparelia et al 2008;Guzman et al 2012).…”

Section: Study On Antibacterial Activitymentioning

confidence: 99%

“…In the wet chemical process, metal nanoparticle is generally synthesized by reducing metal ion (solution phase) using a reducing agent in presence of a stabilizer. To make such synthesis environment friendly, scientists have attempted to use reducing agent as well as stabilizer of biological origin and also to use aqueous phase as a reaction medium (Narayanan and Sakthivel 2011;Kumar et al 2014;Merin et al 2010;Kumari and Philip 2013;MubarakAli et al 2011;Rao and Paria 2013;Jha and Prasad 2010). These green synthesis protocols are not only cost effective but also produce nanocolloid which are very much acceptable for subsequent biological applications.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lippia javanica: a cheap natural source for the synthesis of antibacterial silver nanocolloid

et al. 2015

Self Cite

View full text Add to dashboard Cite

Aqueous silver nanocolloid was synthesized in a single step by a biogenic approach using aqueous leaf extract of Lippia javanica plant which acts as both reducing as well as capping agent. The as-synthesized silver nanoparticles were characterized by UV-visible absorption spectroscopy, high-resolution transmission electron microscopy and Fourier transform infrared spectroscopy (FTIR). The UV-Vis absorption spectra of colloidal silver nanoparticles showed characteristic surface plasmon resonance peak centered at a wavelength of 415 nm. The kinetic study showed that the reduction process was complete within 2 h of time. The TEM analysis showed that most of the particles were spherical in shape and their average diameter was about 17.5 nm. FTIR study confirmed the presence of some organic functional groups in leaf extract and their participation during the reduction as well as stabilization process. In addition, the as-synthesized silver nanoparticles showed antimicrobial activity against clinically isolated pathogenic strain of E. coli and B. subtilis.

show abstract

Section: Study On Antibacterial Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lippia javanica: a cheap natural source for the synthesis of antibacterial silver nanocolloid

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Further, peaks assigned at 1235 and 1069 cm -1 were attributed to C-N stretching possibly due to the presence of amines group. Another intense band at 832 cm -1 was characteristic of the aromatic ring [30][31][32][33].…”

Section: Uv-vis Spectral Studies and Ft-ir Analysismentioning

confidence: 99%

Green synthesis of silver nanoparticles using phlomis leaf extract and investigation of their antibacterial activity

et al. 2016

View full text Add to dashboard Cite

In recent years, the green synthesis of silver nanoparticles using various plant extracts has attracted great attention. This is because, these methods are simple, inexpensive and, eco-friendly. In this study, it was observed that silver ions were reduced by phlomis leaf extract after 5 min, leading to the formation of crystalline silver nanoparticles. Phlomis species is known as a rich source of flavonoids, phenylpropanoids and other phenolic compounds. The silver nanoparticles produced by the phlomis extract were characterized by different techniques including UV-vis spectrophotometry, X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and FT-IR. The SEM and TEM results indicated that AgNPs were predominantly spherical in shape with an average particle size of 25 nm. In addition, the antibacterial activity of biologically synthesized nanopartilcles against Gram-positive (Staphyloccocus aureus and Bacillus cereus) and Gram-negative (Salmonella typhimurium and Escherichia coli) bacteria was proved. This study, therefore, showed that the phlomis leaf extract could be used for the green synthesis of silver nanoparticles with the appropriate antibacterial activity.

show abstract

“…Accordingly, cell division and DNA replications were prevented which ultimately leads to cell death. But in a controversy, Kumar et al [16] have explained the antibacterial mechanisms of Paederia foetida leaves extract mediated AgNPs and demonstrated their in vitro, in vivo DNA damage and from the results concluded that the plant extract mediated AgNPs do not have any genotoxic effect against treated bacterial strains.…”

Section: Introductionmentioning

confidence: 96%

Mechanistic aspects of biologically synthesized silver nanoparticles against food- and water-borne microbes

Krishnaraj

Harper

Choe

et al. 2015

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

In the present study, silver nanoparticles (AgNPs) synthesized from aqueous leaves extract of Malva crispa and their mode of interaction with food- and water-borne microbes were investigated. Formation of AgNPs was conformed through UV-Vis, FE-SEM, EDS, AFM, and HR-TEM analyses. Further the concentration of silver (Ag) in the reaction mixture was conformed through ICP-MS analysis. Different concentration of nanoparticles (1-3 mM) tested to know the inhibitory effect of bacterial pathogens such as Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Salmonella typhi, Salmonella enterica and the fungal pathogens of Penicillium expansum, Penicillium citrinum, Aspergillus oryzae, Aspergillus sojae and Aspergillus niger. Interestingly, nanoparticles synthesized from 2 to 3 mM concentration of AgNO3 showed excellent inhibitory activities against both bacterial and fungal pathogens which are well demonstrated through well diffusion, poison food technique, minimum inhibitory concentration (MIC), and minimum fungicidal concentration (MFC). In addition, mode of interaction of nanoparticles into both bacterial and fungal pathogens was documented through Bio-TEM analysis. Further the genomic DNA isolated from test bacterial strains and their interaction with nanoparticles was carried out to elucidate the possible mode of action of nanoparticles against bacteria. Interestingly, AgNPs did not show any genotoxic effect against all the tested bacterial strains which are pronounced well in agarose gel electrophoresis and for supporting this study, UV-Vis and Bio-TEM analyses were carried out in which no significant changes observed compared with control. Hence, the overall results concluded that the antimicrobial activity of biogenic AgNPs occurred without any DNA damage.

show abstract

Mechanistic study of antibacterial activity of biologically synthesized silver nanocolloids

Cited by 51 publications

References 27 publications

Lippia javanica: a cheap natural source for the synthesis of antibacterial silver nanocolloid

Lippia javanica: a cheap natural source for the synthesis of antibacterial silver nanocolloid

Green synthesis of silver nanoparticles using phlomis leaf extract and investigation of their antibacterial activity

Mechanistic aspects of biologically synthesized silver nanoparticles against food- and water-borne microbes

Contact Info

Product

Resources

About