Mechanistic modeling of in vitro transcription incorporating effects of magnesium pyrophosphate crystallization
Nathan Merica Stover,
Krystian Ganko,
Richard D. Braatz
Abstract:The in vitro transcription (IVT) reaction used in the production of messenger RNA vaccines and therapies remains poorly quantitatively understood. Mechanistic modeling of IVT could inform reaction design, scale‐up, and control. In this work, we develop a mechanistic model of IVT to include nucleation and growth of magnesium pyrophosphate crystals and subsequent agglomeration of crystals and DNA. To help generalize this model to different constructs, a novel quantitative description is included for the rate of … Show more
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