Mechanistic investigation into antibacterial behaviour of suspensions of ZnO nanoparticles against E. coli

Zhang, Lingling; Jiang, Yunhong; Ding, Yulong; Daskalakis, Nikolaos N.; Jeuken, Lars J. C.; Povey, M. J. W.; O’Neill, Alex J.; York, David

doi:10.1007/s11051-009-9711-1

Cited by 418 publications

(245 citation statements)

References 33 publications

Supporting

Mentioning

211

Contrasting

Unclassified

Order By: Relevance

“…In addition, potential antibacterial effects of nanosized ZnO suspensions on bacteria were studied (Tayel et al, 2011Zhang et al, 2010Li et al, 2012;Reddy et al, 2007;Liu et al, 2009). Whilst chronic exposures up to 50 mg/L ZnONP did not affect the sigmoidal growth characteristics of E.coli OP50, strong antibacterial effects were observed at 100 mg/L ZnONP, a finding that was statistically significant (p≤0.05) (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The harmful effects of ZnONPs are driven by their physicochemical properties (dissolution and formation rate, the morphology and chemical composition, surface reactivity, particle number) and the resulting physical damage caused by the aggregation and agglomeration of nanoparticles (Bai et al, 2010;Jiang et al, 2009;Zhang et al, 2010. The bio-kinetic behaviour and in vivo toxicity of ZnONP exposure has, to date, been investigated in several non-mammalian systems including in vitro cell-based assays (Sharma et al, 2012a,b;Ahamed et al, 2011;Wu et al, 2010), bacteria (Li et al, 2011;Reddy et al, 2007), algae (Franklin et al, 2007), plants (Lin and Xing, 2007), crustaceans (Poynton et al, 2011), fish (Bai et al, 2010), earthworms (Hooper et al, 2011) and nematodes (Khare et al, 2011;Ma et al, 2009;Ma et al, 2011;Roh et al, 2009;Wu et al, 2013). The nematode Caenorhabditis elegans, a powerful model organism due to the availability of a completely sequenced genome (Hillier et al, 2005) and many molecular genetics tools has been used in ecotoxicological research to study the molecular to organismal level responses to ROS and heavy metal challenges (Roh et al, 2006;Hughes and Sturzenbaum 2007;Swain et al, 2004Swain et al, , 2010Zeitoun-Ghandour et al, 2010; The roles of the metalloproteins metallothionein (MT) and phytochelatin (PC) are Furthermore, ZnONP mediated toxicity may result from the release of free ionic zinc (George et al 2010;Li et al, 2012;Poynton et al, 2011;Wang et al, 2009), which induces cellular damage via the generation of free reactive oxygen species (ROS), which in turn can promote pro-inflammatory effects Mocchegiani et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Polak

Read

Jurkschat

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

View full text Add to dashboard Cite

Spurgeon, David J.; Sturzenbaum, Stephen R.. 2014. Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegansContact CEH NORA team at noraceh@ceh.ac.ukThe NERC and CEH trademarks and logos ('the Trademarks') are registered trademarks of NERC in the UK and other countries, and may not be used without the prior written consent of the Trademark owner. suggesting that the nominal exposure to pure ZnONPs represents in vivo, at best, a mixture exposure of ionic zinc and nanoparticles. Nevertheless, the analyzes provided evidence that the metallothioneins (mtl-1 and mtl-2), the pytochelatin synthase (pcs-1) and an apoptotic marker (cep-1) were transcriptionally activated. In addition, the DCFH-DA assay provided in vitro evidence of the oxidative potential of ZnONPs in the metal exposure sensitive triple mutant.Raman spectroscopy highlighted that the biomolecular phenotype changes significantly in the metal sensitive knockout worm upon ZnONP exposure, suggesting that these metalloproteins are instrumental in the protection against cytotoxic damage. Finally, ZnONP exposure was shown to decrease growth and development, reproductive capacity and lifespan, effects which were amplified in the triple knockout. By combining diverse toxicological strategies, we identified that individuals (genotypes) housing mutations in key metalloproteins are more susceptible to ZnONP exposure, which underlines the importance of fully functional metalloproteins to minimize ZnONP induced toxicity.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Polak

Read

Jurkschat

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

View full text Add to dashboard Cite

show abstract

“…The metabolic reactions were obtained with catalytic activity. The Nano-sized ZnO suspension clearly has a much higher activity than the micron-sized ZnO suspension (Zhang et al, 2010). However, development of simple and eco-friendly route would help in promoting further interest in the synthesis and application of Zn, Mg and Ti nanoparticles.…”

Section: Biosynthesis Of Zno Npsmentioning

confidence: 99%

Biosynthesis of Zinc Oxide Nanoparticles and Assay of Antibacterial Activity

Ibrahem¹,

Thalij

Saleh

et al. 2017

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

In this study Out of 280 urinary tract infections samples, 212(75.7%) bacterial isolates were recovered. Based on, morphological, cultural and biochemical testes, further, confirmed the results by VITEK 2 System there were 54 (30.2%) of gram positive whereas the 158 (69.8%) of gram negative. The bacterial isolates were distributed as Escherichia coli followed by Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa and Staphylococcus epidermidis which represented 96(45.2%), 48 (22.6%), 43(20.3%), 14 (6.6%) and 11 (5.2%) respectively. The synthesis of Zinc Oxide nanoparticles (ZnO NPs) using biological methods as eco-friendly from the use of Aspergillus niger filtrate in the extracellular synthesis, then identified and characterized by UV-Vis Spectrophotometer and Scanning Electron Microscopy (SEM). Furthermore, determine the antibacterial efficacy of biological synthesized ZnO NPs against isolated pathogens microbes Staphylococcus aureus and Escherichia coli. The results found the used of 100 mL of ZnCL2 in cultivation of Aspergillus niger was obtained and biosynthesis of ZnO NPs at 46 mg. SEM was illustrate the morphology and practices of ZnO NPs sizes and appear as spherical in shape besides, the size range at 41-75 nm. The assurance by the UV-Vis spectrum appears the absorption bands of ZnO NPs at 380 nm. The results of 1.0 and 1.5 mg mL −1 from ZnO NPs synthesized from A. niger were effect of inhibition against S. aureus and E. coli bacterial isolated from urine tracts infection sources. The Inhibition Zone Diameters (IZD) against S. aureus was appear at 24 and 26 mm respectively compared with the IZD from Ciprofloxacin alone at 20 mm. while the same concentration from ZnO NPs against E. coli causing in IZD at 25 and 28 mm respectively compared with Ciprofloxacin inhibition alone at 23 mm.

show abstract

“…But the active anionic molecules are effective toxic to microbial substance. In addition, the damage of cell membrane might directly lead to the leakage of minerals, proteins and genetic materials causing ultimate cell death 28,29 . The mechanism involved in the generation of reactive oxygen species from CeO 2 nanoparticles induced by UV light.…”

Section: Antimicrobial Studiesmentioning

confidence: 99%

Synthesis and Characterization of Various Metal Ions Doped CeO2 Nanoparticles Derived from the Azadirachta Indica Leaf Extracts

2017

Chem Sci Trans

View full text Add to dashboard Cite

Abstract:The cerium oxide nanoparticles (CeO 2 NPs) and M 2+ doped CeO 2 NPs (M 2+ = Zn 2+ , Ni 2+ , Cu 2+ and Co 2+ ) were prepared through the Azadirachta indica (A. indica) leaf extracts. The x-ray diffraction studies confirmed that synthesized pure and M 2+ doped CeO 2 NPs were exhibited the cubic structure. The FESEM and TEM images of both pure and M 2+ doped CeO 2 NPs have exhibited spherical structure. From the EDAX spectral analysis, the elemental compositions were identified. The various functional groups were confirmed by FT-IR spectrum. Photoluminescence spectral analysis, small shift observed for emission of the CeO 2 NPs values as compared to that of the transition metal ions doped CeO 2 NPs emission values. The antimicrobial studies were performed against a set of gram positive (Staphylococcus aureus and Streptococcus pneumoniae) and gram negative (Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumonia and Shigelladysenteriae) bacterial and fungal candida albicans strain.

show abstract

Mechanistic investigation into antibacterial behaviour of suspensions of ZnO nanoparticles against E. coli

Cited by 418 publications

References 33 publications

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Biosynthesis of Zinc Oxide Nanoparticles and Assay of Antibacterial Activity

Synthesis and Characterization of Various Metal Ions Doped CeO2 Nanoparticles Derived from the Azadirachta Indica Leaf Extracts

Contact Info

Product

Resources

About