Mechanistic Insights into the Release of Doxorubicin from Graphene Oxide in Cancer Cells

Quagliarini, Erica; Santo, Riccardo Di; Pozzi, Daniela; Tentori, Paolo; Cardarelli, Francesco; Caracciolo, Giulio

doi:10.3390/nano10081482

Cited by 24 publications

(18 citation statements)

References 39 publications

(44 reference statements)

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“…Another promising DOX delivery system, called DOX-loaded graphene oxide (GO) or GO-DOX, has exhibited much higher anti-cancer efficiency than L-DOX. FLIM determined that the mechanism behind this improvement was that GO-DOX bound to cell plasma membranes to induce massive intracellular DOX release [ 128 ]. Additionally, a novel DOX delivery system, called PAH-Cit-Dox, has also been constructed, as shown in Fig.…”

Section: Flim Combined With Fluorophore Dye Probesmentioning

confidence: 99%

FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring

et al. 2021

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Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past 30 years and widely applied in biomedical engineering. Recent progress in fluorophore-dyed probe design has widened the application prospects of fluorescence. Because fluorescence lifetime is sensitive to microenvironments and molecule alterations, FLIM is promising for the detection of pathological conditions. Current cancer-related FLIM applications can be divided into three main categories: (i) FLIM with autofluorescence molecules in or out of a cell, especially with reduced form of nicotinamide adenine dinucleotide, and flavin adenine dinucleotide for cellular metabolism research; (ii) FLIM with Förster resonance energy transfer for monitoring protein interactions; and (iii) FLIM with fluorophore-dyed probes for specific aberration detection. Advancements in nanomaterial production and efficient calculation systems, as well as novel cancer biomarker discoveries, have promoted FLIM optimization, offering more opportunities for medical research and applications to cancer diagnosis and treatment monitoring. This review summarizes cutting-edge researches from 2015 to 2020 on cancer-related FLIM applications and the potential of FLIM for future cancer diagnosis methods and anti-cancer therapy development. We also highlight current challenges and provide perspectives for further investigation.

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Section: Flim Combined With Fluorophore Dye Probesmentioning

confidence: 99%

FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring

et al. 2021

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“…The GO solution was then centrifuged at 15,000× g for 100 min (Hermle Z 216 MK, Hermle Labortechnik). Lastly, the supernatant was recovered for subsequent use and its concentration (30 μg mL −1 ) was determined by UV-VIS experiments [ 49 ].…”

Section: Methodsmentioning

confidence: 99%

Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic Cancer

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Cascone

et al. 2020

Cancers

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Simultaneous detection of multiple analytes from a single biological sample is gaining more attention in the development of more reliable and point-of-care diagnostic devices. We developed a multiplexed strategy that combined outcomes of clinical biomarkers with analysis of the protein corona that forms around graphene oxide sheets upon exposure to patient’s plasma. As a paradigmatic case study, we selected pancreatic ductal adenocarcinoma (PDAC), mainly because of the absence of effective detection strategies that resulted in an extremely low five-year survival rate after diagnosis (<10%). Association of protein corona analysis and haemoglobin levels discriminated PDAC patients from healthy volunteers in up to 90% of cases. If further confirmed in larger-cohort studies, this approach may be used in the detection of PDAC.

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“…The GO-DOX exhibited much higher anticancer activities when administered to cellular models of breast cancer. Through live-cell confocal imaging and fluorescence lifetime imaging microscopy, researchers found that GO-DOX achieved its high efficacy by inducing massive intracellular DOX release when bonded to the cell plasma membrane [ 137 ]. Many research indicates that GOs and rGOs can target at hypoxia [ 138 ] and abnormal angiogenesis in cancer TME [ 139 , 140 ].…”

Section: Nanomaterials Used For Cancer Treatmentmentioning

confidence: 99%

Nanomaterials for cancer therapy: current progress and perspectives

et al. 2021

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Cancer is a disease with complex pathological process. Current chemotherapy faces problems such as lack of specificity, cytotoxicity, induction of multi-drug resistance and stem-like cells growth. Nanomaterials are materials in the nanorange 1–100 nm which possess unique optical, magnetic, and electrical properties. Nanomaterials used in cancer therapy can be classified into several main categories. Targeting cancer cells, tumor microenvironment, and immune system, these nanomaterials have been modified for a wide range of cancer therapies to overcome toxicity and lack of specificity, enhance drug capacity as well as bioavailability. Although the number of studies has been increasing, the number of approved nano-drugs has not increased much over the years. To better improve clinical translation, further research is needed for targeted drug delivery by nano-carriers to reduce toxicity, enhance permeability and retention effects, and minimize the shielding effect of protein corona. This review summarizes novel nanomaterials fabricated in research and clinical use, discusses current limitations and obstacles that hinder the translation from research to clinical use, and provides suggestions for more efficient adoption of nanomaterials in cancer therapy.

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Mechanistic Insights into the Release of Doxorubicin from Graphene Oxide in Cancer Cells

Cited by 24 publications

References 39 publications

FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring

FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring

Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic Cancer

Nanomaterials for cancer therapy: current progress and perspectives

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