Mechanistic insights into Schizosaccharomyces pombe GT-A family protein Pvg3 in the biosynthesis of pyruvylated β1,3-galactose of N-linked oligosaccharides

Identification of a putative α-galactoside β-(1 → 3)-galactosyltransferase involved in the biosynthesis of galactomannan side chain of glucuronoxylomannogalactan in Cryptococcus neoformans

“…Another hypothesis is that Ggt2 does not exhibit glycosyltransferase activity by itself. For example, S. pombe Pvg3, which belongs to the GT31 family, is not active alone but exhibits glycosyltransferase activity by forming a complex with several proteins ( Fukunaga et al, 2023 ). The enzymatic features of Ggt2 may be essential for the formation of the unique GXMGal structure and should be studied in more detail.…”

“…However, GXMGal biosynthesis-related glycosyltransferases are limited to only two species, β-(1 → 2)-xyltransferases Cxt1 and Cxt2 ( Klutts et al, 2007 ; Klutts and Doering, 2008 ; Reilly et al, 2009 ; Wang et al, 2018 ). The putative α-Gal β-(1 → 3)-Gal transferase Pvg3 in Schizosaccharomyces pombe , which belongs to the GT31 family, highlights the diversity of glycosyltransferases ( Andreishcheva et al, 2004 ; Fukunaga et al, 2023 ). In C. neoformans , the genome encodes three GT31 family glycosyltransferases, one of which, Ggt1, functions as an α-Man β-(1 → 6)-Gal transferase in GIPC biosynthesis ( Wohlschlager et al, 2013 ).…”