Mechanistic insights into hormesis induced by erythromycin in the marine alga Thalassiosira weissflogii

Mo, Jiezhang; Lv, Runnan; Qin, Xian; Wu, Xintong; Chen, Haibo; Yan, Neng; Shi, Jingchun; Wu, Yinglin; Liu, Wenhua; Kong, Richard Yuen Chong; Guo, Jiahua

doi:10.1016/j.ecoenv.2023.115242

Cited by 9 publications

(17 citation statements)

References 61 publications

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“…The growth of T. weissflogii under erythromycin stress is shown in Figure 1. The results presented here were in accordance with the published data [34], where a hormetic effect was observed in T. weissflogii under erythromycin stress. Specifically, the algal cells of the 0.001, 0.75, and 2.5 mg/L erythromycin groups were 112.1% ± 2.4%, 75.6% ± 2.5%, and 47.7% ± 1.8% of those of the control (Figure 1).…”

Section: Erythromycin Exposure Modified the Growth Of Marine Microalgasupporting

confidence: 93%

“…However, barely any ecotoxicological information on antibiotics in this species is available. Most recently, a hormetic effect (algal growth was promoted in low-dose treatments but inhibited at high-dose exposures) of erythromycin on T. weissflogii was reported, in which the molecular signaling pathways and biological processes related to xenobiotic metabolism, ribosome assembly, protein translation, photosynthesis, and oxidative stress were modified [34]; however, these transcriptional and physiological changes have yet to be validated at the metabolic level. Nonetheless, it has been proposed that the mechanisms underlying growth promotion induced by low-level exposure to chemicals/pollutants are attributed to an increase in ROS production, pigment contents, protein synthesis, and possibly the accumulation of phytohormones [35,36].…”

Section: Introductionmentioning

confidence: 99%

“…Based on recently published transcriptomic data [34], this study hypothesizes that erythromycin treatments modify the metabolic pathways involved in ribosome assembly, amino acid biosynthesis and/or metabolism, and photosynthesis. We investigated the effects of erythromycin stress on T. weissflogii by analyzing its metabolomic (non-targeted metabolome) and biochemical responses, such as its mitochondrial membrane potential (MMP) and cell membrane integrity.…”

Section: Introductionmentioning

confidence: 99%

“…We investigated the effects of erythromycin stress on T. weissflogii by analyzing its metabolomic (non-targeted metabolome) and biochemical responses, such as its mitochondrial membrane potential (MMP) and cell membrane integrity. To validate the transcriptomic alterations in T. weissflogii under erythromycin stress, the metabolomic data generated in the present study were conjointly analyzed with the published transcriptomic data [34]. This study aimed to gain a better understanding of erythromycin ecotoxicity in marine algae, identify potential cellular targets, and develop biomarkers for erythromycin toxicity in marine diatoms.…”

Section: Introductionmentioning

confidence: 99%

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Metabolomic Response of Thalassiosira weissflogii to Erythromycin Stress: Detoxification Systems, Steroidal Metabolites, and Energy Metabolism

Wu,

Tong,

et al. 2024

Plants

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Erythromycin, a macrolide antibiotic, is a prioritized pollutant that poses a high risk to environmental health. It has been detected in different environmental matrices and can cause undesired effects in aquatic organisms, particularly freshwater algae, which are primary producers. However, the impact of erythromycin on marine algae remains largely unexplored. Erythromycin has been reported to induce hormetic effects in the marine diatom Thalassiosira weissflogii (T. weissflogii). These effects are associated with the molecular pathways and biological processes of ribosome assembly, protein translation, photosynthesis, and oxidative stress. However, the alterations in the global gene expression have yet to be validated at the metabolic level. The present study used non-targeted metabolomic analysis to reveal the altered metabolic profiles of T. weissflogii under erythromycin stress. The results showed that the increased cell density was possibly attributed to the accumulation of steroidal compounds with potential hormonic action at the metabolic level. Additionally, slight increases in the mitochondrial membrane potential (MMP) and viable cells were observed in the treatment of 0.001 mg/L of erythromycin (an environmentally realistic level). Contrarily, the 0.75 and 2.5 mg/L erythromycin treatments (corresponding to EC20 and EC50, respectively) showed decreases in the MMP, cell density, and viable algal cells, which were associated with modified metabolic pathways involving ATP-binding cassette (ABC) transporters, the metabolism of hydrocarbons and lipids, thiamine metabolism, and the metabolism of porphyrin and chlorophyll. These findings suggest that metabolomic analysis, as a complement to the measurement of apical endpoints, could provide novel insights into the molecular mechanisms of hormesis induced by antibiotic agents in algae.

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Section: Erythromycin Exposure Modified the Growth Of Marine Microalgasupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolomic Response of Thalassiosira weissflogii to Erythromycin Stress: Detoxification Systems, Steroidal Metabolites, and Energy Metabolism

Wu,

Tong,

et al. 2024

Plants

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“…Feng’s study showed that β-lactam antibiotics, to a certain extent, delayed the time of the first laying of fleas and also the time of the first egg conception in Daphnia magna [ 8 ]. Pharmaceuticals have adverse effects on the growth and metabolism of microalgae, where they can disrupt photosynthetic and gene expression processes, increase the formation of reactive oxygen species, and change the activities of antioxidant enzymes [ 11 , 12 , 13 ]. The impact of these pharmaceuticals on the environment are more concerning, considering that they do not appear individually, but rather as a complex mixture, potentially leading to unwanted synergistic effects [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Mechanism of the Synergistic Toxicity of Ampicillin and Cefazoline on Selenastrum capricornutum

Huang,

Qin,

et al. 2024

Toxics

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Ampicillin (AMP) and cefazolin (CZO) are commonly used β-lactam antibiotics which are extensively globally produced. Additionally, AMP and CZO are known to have relatively high ecotoxicity. Notably, the mix of AMP and CZO creates a synergistic effect that is more harmful to the environment, and how exposure to AMP-CZO can induce synergism in algae remains virtually unknown. To yield comprehensive mechanistic insights into chemical toxicity, including dose–response relationships and variations in species sensitivity, the integration of multiple endpoints with de novo transcriptomics analyses were used in this study. We employed Selenastrum capricornutum to investigate its toxicological responses to AMP and CZO at various biological levels, with the aim of elucidating the underlying mechanisms. Our assessment of multiple endpoints revealed a significant growth inhibition in response to AMP at the relevant concentrations. This inhibition was associated with increased levels of reactive oxygen species (ROS) and perturbations in nitrogen metabolism, carbohydrate metabolism, and energy metabolism. Growth inhibition in the presence of CZO and the AMP-CZO combination was linked to reduced viability levels, elevated ROS production, decreased total soluble protein content, inhibited photosynthesis, and disruptions in the key signaling pathways related to starch and sucrose metabolism, ribosome function, amino acid biosynthesis, and the production of secondary metabolites. It was concluded from the physiological level that the synergistic effect of Chlorophyll a (Chla) and Superoxide dismutase (SOD) activity strengthened the growth inhibition of S. capricornutum in the AMP-CZO synergistic group. According to the results of transcriptomic analysis, the simultaneous down-regulation of LHCA4, LHCA1, LHCA5, and sodA destroyed the functions of the photosynthetic system and the antioxidant system, respectively. Such information is invaluable for environmental risk assessments. The results provided critical knowledge for a better understanding of the potential ecological impacts of these antibiotics on non-target organisms.

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Transcriptomics revealed the key molecular mechanisms of ofloxacin-induced hormesis in Chlorella pyrenoidosa at environmentally relevant concentration

Zhou,

Zhu,

et al. 2024

Environmental Pollution

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Mechanistic insights into hormesis induced by erythromycin in the marine alga Thalassiosira weissflogii

Cited by 9 publications

References 61 publications

Metabolomic Response of Thalassiosira weissflogii to Erythromycin Stress: Detoxification Systems, Steroidal Metabolites, and Energy Metabolism

Metabolomic Response of Thalassiosira weissflogii to Erythromycin Stress: Detoxification Systems, Steroidal Metabolites, and Energy Metabolism

Mechanism of the Synergistic Toxicity of Ampicillin and Cefazoline on Selenastrum capricornutum

Transcriptomics revealed the key molecular mechanisms of ofloxacin-induced hormesis in Chlorella pyrenoidosa at environmentally relevant concentration

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