Mechanistic Insight into Permeation of Plasma-Generated Species from Vacuum into Water Bulk

Razzokov, Jamoliddin; Fazliev, Sunnatullo; Kodirov, Akbar; Attri, Pankaj; Chen, Zhitong; Shiratani, Masaharu

doi:10.3390/ijms23116330

Cited by 8 publications

(6 citation statements)

References 72 publications

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“…A previous study also reported shorter streptococcal chains of S. mutans after CAP treatment compared with the pre-treatment length [11]. During plasma production, oxidants like hydrogen peroxide (H 2 O 2 ) accumulate at the vacuum-water boundary and within the water itself [40], implying that oxidants can infiltrate liquids. Thus, the RONS generated by CAP may reach the biofilm, potentially undermining the structural integrity of EPSs and leading to cell damage [41].…”

Section: Discussionmentioning

confidence: 94%

Efficacy of Cold Atmospheric Plasma on Pathogenicity of Oral Microcosm Biofilms

Kim

2024

Applied Sciences

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This study aimed to compare the longitudinal efficacy between chlorhexidine gluconate (CHX; 0.12%) and cold atmospheric plasma (CAP) in reducing oral biofilm pathogenicity, utilizing a quantitative light-induced fluorescence-digital (QLF-D) camera. Oral microcosm biofilms were developed for 2 days on 57 hydroxyapatite disks. These biofilms were treated with distilled water for 1 min, CHX for 1 min, and CAP for 2 min over the course of 6 days. The red fluorescence intensities of the biofilms were measured using a QLF-D and expressed as pre- and post-treatment red/green ratios (RatioR/G). The bacterial viability (ratio of the green-stained area to the total stained area, RatioG/G+R) was calculated using live/dead bacterial staining; the total and aciduric bacterial counts were determined. A significant intergroup difference was found between RatioR/G changes according to the treatment period (p < 0.001). The RatioR/G observed within the CAP-treated group was significantly lower compared with the CHX-treated group at every interval of measurement (p < 0.001). The CAP-treated group also exhibited a lower RatioG/G+R and more weakened bacterial aggregation compared with the CHX-treated group (p < 0.05). In the group treated with CAP, the counts of both total and aciduric bacteria were substantially reduced compared with the DW group, with a statistically significant reduction (p < 0.001). Therefore, CAP may be more effective in minimizing oral microcosm biofilm pathogenicity than 0.12% CHX.

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Section: Discussionmentioning

confidence: 94%

Efficacy of Cold Atmospheric Plasma on Pathogenicity of Oral Microcosm Biofilms

Kim

2024

Applied Sciences

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“…Although this previous study did not explicitly examine biofilms, the shared characteristics of peptide and EPS aggregation suggest that the oxidizing effects of CAP may impede EPS cohesiveness within biofilm structures. Additionally, based on the reported concentrations of oxidizing agents, such as H 2 O 2 at the vacuum-water interface and within the water bulk during plasma generation [41], plasma-generated substances can permeate into liquids. These findings suggest that the reactive oxygen species (ROS) produced by CAP can be delivered to biofilms, potentially destabilizing EPS structure and causing cellular destruction [42].…”

Section: Discussionmentioning

confidence: 99%

Influence of Biofilm Maturity on the Antibacterial Efficacy of Cold Atmospheric Plasma in Oral Microcosm Biofilms

Kim

2024

Biomedicines

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As biofilms mature, biomass and extracellular polysaccharide (EPS) content increases, enhancing pathogenicity. Therefore, this study aimed to evaluate the antibacterial efficacy of cold atmospheric plasma (CAP) against oral microcosm biofilms and the influence of biofilm maturity on treatment. Oral microcosm biofilms were cultured on hydroxyapatite disks for 2 and 6 days. Based on the treatment and biofilm maturity, these were subsequently allocated into six groups (N = 19 each): Groups 1 and 2 were incubated with distilled water for 1 min; Groups 3 and 4 were treated with CAP for 2 min, and Groups 5 and 6 were treated with 0.12% chlorhexidine gluconate for 1 min. Groups 1, 3, and 5 represent 2-day biofilms, and Groups 2, 4, and 6 represent 6-day biofilms. Treatments were repeated daily for 5 days. Antibacterial efficacy was analyzed by measuring oral biofilms’ red fluorescence intensity (RatioR/G) and quantifying EPS content and bacterial viability. The RatioR/G was 1.089-fold and 1.104-fold higher in Groups 4 and 6 than in Groups 3 and 5 following antibacterial treatment, respectively (p < 0.001). EPS content increased by 1.71-fold in Group 6 than in Group 5 (p < 0.001). Bacterial survival rate was the lowest in Group 3 (p = 0.005). These findings underscore the relevance of CAP treatment in maintaining antibacterial efficacy regardless of the biofilm development stage, highlighting its potential utility in oral care.

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“…The generated during CAP treatment initially interact with the water on the cell surface, before affecting the cell membrane [20]. Subsequently, RONS penetrate into the cell, inducing oxidative stress in the membrane, thereby forming lipid peroxide as the primary oxidation product and aldehyde, alcohol, and ketone as secondary products [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes

Abduvokhidov,

Yusupov,

Shahzad

et al. 2023

Biomolecules

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The potential of cold atmospheric plasma (CAP) in biomedical applications has received significant interest, due to its ability to generate reactive oxygen and nitrogen species (RONS). Upon exposure to living cells, CAP triggers alterations in various cellular components, such as the cell membrane. However, the permeation of RONS across nitrated and oxidized membranes remains understudied. To address this gap, we conducted molecular dynamics simulations, to investigate the permeation capabilities of RONS across modified cell membranes. This computational study investigated the translocation processes of less hydrophilic and hydrophilic RONS across the phospholipid bilayer (PLB), with various degrees of oxidation and nitration, and elucidated the impact of RONS on PLB permeability. The simulation results showed that less hydrophilic species, i.e., NO, NO2, N2O4, and O3, have a higher penetration ability through nitro-oxidized PLB compared to hydrophilic RONS, i.e., HNO3, s-cis-HONO, s-trans-HONO, H2O2, HO2, and OH. In particular, nitro-oxidation of PLB, induced by, e.g., cold atmospheric plasma, has minimal impact on the penetration of free energy barriers of less hydrophilic species, while it lowers these barriers for hydrophilic RONS, thereby enhancing their translocation across nitro-oxidized PLB. This research contributes to a better understanding of the translocation abilities of RONS in the field of plasma biomedical applications and highlights the need for further analysis of their role in intracellular signaling pathways.

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Mechanistic Insight into Permeation of Plasma-Generated Species from Vacuum into Water Bulk

Cited by 8 publications

References 72 publications

Efficacy of Cold Atmospheric Plasma on Pathogenicity of Oral Microcosm Biofilms

Efficacy of Cold Atmospheric Plasma on Pathogenicity of Oral Microcosm Biofilms

Influence of Biofilm Maturity on the Antibacterial Efficacy of Cold Atmospheric Plasma in Oral Microcosm Biofilms

Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes

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