Mechanistic Insight into Nanoparticle Surface Adsorption by Solution NMR Spectroscopy in an Aqueous Gel

Egner, Timothy K.; Naik, Pranjali; Nelson, Nicholas C.; Slowing, Igor I.; Venditti, Vincenzo

doi:10.1002/anie.201704471

Cited by 32 publications

(53 citation statements)

References 25 publications

(26 reference statements)

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“…Because of this, it is challenging to generalize the behavior of biomolecule‐AuNP association, and for any NP system, subtle changes to surface chemistry can lead to large changes in binding behavior, altering both the soft and hard corona composition and dynamics. Most proteins bind to AuNPs spontaneously without causing any aggregation, and when aggregation does occur, hydrogels can be used to maintain NPs in suspension . Thus, AuNPs provide a good platform to study NP‐protein interactions to gain insight on orientation, structure, and function of the protein .…”

Section: Results From Different Nanoparticle Systemsmentioning

confidence: 99%

“…Stated differently, for NPs smaller than 30 nm, DEST and

Δ R_{2}^{o b s}

can only determine the product of

p_{B} R_{2}^{N P}

without the collection of

C_{f a s t}^{m a x}

. Thus, in practice, DEST has been applied to liposomal NPs and ceria NPs, but it cannot be used for the proteins tested with AuNPs thus far, as the exchange rate is too slow …”

Section: Solution Nmr Approaches For Nanoparticle Studiesmentioning

confidence: 99%

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Protein Interactions with Nanoparticle Surfaces: Highlighting Solution NMR Techniques

Perera

Hill

Fitzkee

2019

Israel Journal of Chemistry

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In the last decade, nanoparticles (NPs) have become a key tool in medicine and biotechnology as drug delivery systems, biosensors and diagnostic devices. The composition and surface chemistry of NPs vary based on the materials used: typically organic polymers, inorganic materials, or lipids. Nanoparticle classes can be further divided into sub-categories depending on the surface modification and functionalization. These surface properties matter when NPs are introduced into a physiological environment, as they will influence how nucleic acids, lipids, and proteins will interact with the NP surface. While small-molecule interactions are easily probed using NMR spectroscopy, studying protein-NP interactions using NMR introduces several challenges. For example, globular proteins may have a perturbed conforma-tion when attached to a foreign surface, and the size of NPprotein conjugates can lead to excessive line broadening. Many of these challenges have been addressed, and NMR spectroscopy is becoming a mature technique for in situ analysis of NP binding behavior. It is therefore not surprising that NMR has been applied to NP systems and has been used to study biomolecules on NP surfaces. Important considerations include corona composition, protein behavior, and ligand architecture. These features are difficult to resolve using classical surface and material characterization strategies, and NMR provides a complementary avenue of characterization. In this review, we examine how solution NMR can be combined with other analytical techniques to investigate protein behavior on NP surfaces.

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Section: Results From Different Nanoparticle Systemsmentioning

confidence: 99%

“…Stated differently, for NPs smaller than 30 nm, DEST and

Δ R_{2}^{o b s}

can only determine the product of

p_{B} R_{2}^{N P}

without the collection of

C_{f a s t}^{m a x}

. Thus, in practice, DEST has been applied to liposomal NPs and ceria NPs, but it cannot be used for the proteins tested with AuNPs thus far, as the exchange rate is too slow …”

Section: Solution Nmr Approaches For Nanoparticle Studiesmentioning

confidence: 99%

Protein Interactions with Nanoparticle Surfaces: Highlighting Solution NMR Techniques

Perera

Hill

Fitzkee

2019

Israel Journal of Chemistry

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“…Currently, nuclear magnetic resonance (NMR) is a crucial technique to characterize the surface chemistry of as-synthesized NPs or those aer carrying out ligand exchange. [5][6][7][8] In addition, 2D NMR techniques (i.e. two dimensional nuclear Overhauser effect (NOESY) experiment) 1,4 and saturation-transfer difference (STD)-NMR 9 are useful approaches for the complete characterization of NP surface chemistry.…”

Section: Introductionmentioning

confidence: 99%

Using evolved gas analysis – mass spectrometry to characterize adsorption on a nanoparticle surface

et al. 2019

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“…Nuclear Magnetic Resonance (NMR) is the most versatile tool for determining molecular structure. NMR is being increasingly used in recent years to examine molecules that interact noncovalently with nanoparticle surfaces . Previously, we have used Saturation‐Transfer Difference (STD) NMR to examine the binding between small molecules and the surface of polystyrene (PS) nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Interaction between cyanine dye IR‐783 and polystyrene nanoparticles in solution

Zhang

Casabianca

2018

Magnetic Reson in Chemistry

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The interactions between small molecule drugs or dyes and nanoparticles are important to the use of nanoparticles in medicine. Noncovalent adsorption of dyes on nanoparticle surfaces is also important to the development of nanoparticle dual-use imaging contrast agents. In this work, solution-state NMR is used to examine the noncovalent interaction between a near-infrared cyanine dye and the surface of polystyrene nanoparticles in solution. Using 1D proton NMR, we can approximate the number of dye molecules that associate with each nanoparticle for different sized nanoparticles. Saturation-Transfer Difference NMR was also used to show that protons near the positively charged nitrogen in the dye are more strongly associated with the negatively charged nanoparticle surface than protons near the negatively charged sulfate groups of the dye. The methods described here can be used to study similar drug or dye molecules interacting with the surface of organic nanoparticles.

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Mechanistic Insight into Nanoparticle Surface Adsorption by Solution NMR Spectroscopy in an Aqueous Gel

Cited by 32 publications

References 25 publications

Protein Interactions with Nanoparticle Surfaces: Highlighting Solution NMR Techniques

Protein Interactions with Nanoparticle Surfaces: Highlighting Solution NMR Techniques

Using evolved gas analysis – mass spectrometry to characterize adsorption on a nanoparticle surface

Interaction between cyanine dye IR‐783 and polystyrene nanoparticles in solution

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