Mechanistic and Technical Challenges in Studying the Human Microbiome and Cancer Epidemiology

Verma, Mukesh

doi:10.1177/1533034616645219

Cited by 11 publications

(10 citation statements)

References 92 publications

(127 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The predominant oral microbial communities belonged to Firmicutes (genus Streptococcus , family Veillonellaceae, genus Granulicatella ), Proteobacteria (genera Neisseria , Haemophilus ), Actinobacteria (genera Corynebacterium , Rothia , Actinomyces ), Bacteroidetes (genera Prevotella , Capnocytophaga , and Porphyromonas ), and Fusobacteria (genus Fusobacterium ) are observed in healthy individuals. 31,32…”

Section: Oral Microbiome Signatures In Healthy Individualsmentioning

confidence: 99%

Role of Oral Microbiome Signatures in Diagnosis and Prognosis of Oral Cancer

Chattopadhyay

Verma

Panda

2019

Technol Cancer Res Treat

Self Cite

225

175

View full text Add to dashboard Cite

Despite advancement in cancer treatment, oral cancer has a poor prognosis and is often detected at late stage. To overcome these challenges, investigators should search for early diagnostic and prognostic biomarkers. More than 700 bacterial species reside in the oral cavity. The oral microbiome population varies by saliva and different habitats of oral cavity. Tobacco, alcohol, and betel nut, which are causative factors of oral cancer, may alter the oral microbiome composition. Both pathogenic and commensal strains of bacteria have significantly contributed to oral cancer. Numerous bacterial species in the oral cavity are involved in chronic inflammation that lead to development of oral carcinogenesis. Bacterial products and its metabolic by-products may induce permanent genetic alterations in epithelial cells of the host that drive proliferation and/or survival of epithelial cells. Porphyromonas gingivalis and Fusobacterium nucleatum induce production of inflammatory cytokines, cell proliferation, and inhibition of apoptosis, cellular invasion, and migration thorough host cell genomic alterations. Recent advancement in metagenomic technologies may be useful in identifying oral cancer–related microbiome, their genomes, virulence properties, and their interaction with host immunity. It is very important to address which bacterial species is responsible for driving oral carcinogenesis. Alteration in the oral commensal microbial communities have potential application as a diagnostic tool to predict oral squamous cell carcinoma. Clinicians should be aware that the protective properties of the resident microflora are beneficial to define treatment strategies. To develop highly precise and effective therapeutic approaches, identification of specific oral microbiomes may be required. In this review, we narrate the role of microbiome in the progression of oral cancer and its role as an early diagnostic and prognostic biomarker for oral cancer.

show abstract

Section: Oral Microbiome Signatures In Healthy Individualsmentioning

confidence: 99%

Role of Oral Microbiome Signatures in Diagnosis and Prognosis of Oral Cancer

Chattopadhyay

Verma

Panda

2019

Technol Cancer Res Treat

Self Cite

225

175

View full text Add to dashboard Cite

show abstract

“…Research studies indicate that composition and functionality of the gut microbiome play a major role in modulating CRC risk [ 12 , 21 – 31 ]. Studies seeking to identify specific bacterial signatures associated with cancer incidence have not been successful [ 32 – 34 ] due to the complexity of the gut microbiome [ 35 – 38 ], and the diversity within and between individuals and populations [ 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

Gut microbiome of Moroccan colorectal cancer patients

Allali

Benkeblia

Bouguenouch

et al. 2018

Med Microbiol Immunol

View full text Add to dashboard Cite

Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population. The aim of our study was to compare the stool microbiome of Moroccan cancer patients with healthy individuals. We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing. Principal coordinate analysis of samples revealed defined cancer versus healthy clusters. Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06). Porphyromonas, Clostridium, Ruminococcus, Selenomonas, and Fusobacterium were significantly overrepresented in diseased patients, similarly to other studies. Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls. This suggests that involvement of these functional metagenomes is similar and relevant in the carcinogenesis process, independent of the origin of the samples. Results from this study allowed identification of bacterial taxa relevant to the Moroccan population and encourages larger studies to facilitate population-directed therapeutic approaches.Electronic supplementary materialThe online version of this article (10.1007/s00430-018-0542-5) contains supplementary material, which is available to authorized users.

show abstract

“…Most of the reviewed studies used multimodal data (see Table 1 ). Each type of data has its advantages and disadvantages concerning its costs, the data it provides or the difficulty of being quantified [ 18 , 20 , 21 , 22 , 23 , 24 , 28 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ].…”

Section: Resultsmentioning

confidence: 99%

Methods for Stratification and Validation Cohorts: A Scoping Review

Moral

Sánchez-Niubò²,

Monistrol-Mula³

et al. 2022

JPM

View full text Add to dashboard Cite

Personalized medicine requires large cohorts for patient stratification and validation of patient clustering. However, standards and harmonized practices on the methods and tools to be used for the design and management of cohorts in personalized medicine remain to be defined. This study aims to describe the current state-of-the-art in this area. A scoping review was conducted searching in PubMed, EMBASE, Web of Science, Psycinfo and Cochrane Library for reviews about tools and methods related to cohorts used in personalized medicine. The search focused on cancer, stroke and Alzheimer’s disease and was limited to reports in English, French, German, Italian and Spanish published from 2005 to April 2020. The screening process was reported through a PRISMA flowchart. Fifty reviews were included, mostly including information about how data were generated (25/50) and about tools used for data management and analysis (24/50). No direct information was found about the quality of data and the requirements to monitor associated clinical data. A scarcity of information and standards was found in specific areas such as sample size calculation. With this information, comprehensive guidelines could be developed in the future to improve the reproducibility and robustness in the design and management of cohorts in personalized medicine studies.

show abstract

Mechanistic and Technical Challenges in Studying the Human Microbiome and Cancer Epidemiology

Cited by 11 publications

References 92 publications

Role of Oral Microbiome Signatures in Diagnosis and Prognosis of Oral Cancer

Role of Oral Microbiome Signatures in Diagnosis and Prognosis of Oral Cancer

Gut microbiome of Moroccan colorectal cancer patients

Methods for Stratification and Validation Cohorts: A Scoping Review

Contact Info

Product

Resources

About