Mechanisms underlying the wound healing potential of propolis based on its in vitro antioxidant activity

Cao, Xueping; Chen, Yi-Fan; Zhang, Jianglin; You, Mengmeng; Wang, Kai

doi:10.1016/j.phymed.2017.06.001

Cited by 57 publications

(52 citation statements)

References 43 publications

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“…The chemical composition of propolis is mainly influenced by the vegetation at the region of collection. [38] The differences in the chemical composition of propolis samples lead to variations in bioactivities exerted by them. Therefore, the chemical profiling as well as the quantifying the marker components of the propolis extracts that are used in bioassays is essential.…”

Section: Protein Expression Analysismentioning

confidence: 99%

“…The previously applied method was used during the HPTLC analysis. [38] Accordingly, propolis sample test solution (5 μL) and each standard solution (2 μL) was applied on HPTLC glass plates (20 × 10 cm) covered with silica gel 60 F 254 using a Linomat V automatic sample spotter fitted with 100 mL Hamilton syringe. For the separation, 10 mL of the mobile phase containing hexane/ethyl acetate/glacial acetic acid (5 : 3 : 1, v/v/v) and 37 % hydrochloric acid for saturation were applied.…”

Section: Evaluation Of Protein Expression With Flow Cytometrymentioning

confidence: 99%

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Antiproliferative Activity of Chemically Characterized Propolis from Turkey and Its Mechanisms of Action

Aru

Güzelmeriç

Akgül

et al. 2019

Chemistry & Biodiversity

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The aim of this study was to evaluate the ethanolic extract of propolis originated from northern Turkey for its antiproliferative, apoptotic and cell cycle arrest promoting effects on MCF7, HGC27, A549 cancer cell lines and a healthy cell line (HUVEC) in terms of DNA content, morphological features, expression of cell cycle checkpoint proteins p21, p53, Cyclin D1 and immune checkpoint protein PD‐L1. The extract showed moderate antiproliferative activity against all tested cancer cell lines with IC50 values in the range of 58.6–90.7 μg/mL in MTS assay. Further studies indicated that propolis extract exerted apoptotic effect on cancer cell lines, promoted cell cycle arrest through activation of p21 and resulted in accumulation at G0/G1 phase of cancer cells. Propolis treatment caused increased cell size, according to fluorescent imaging except for MCF7. HPTLC analysis revealed that 3‐O‐methylquercetin, chrysin, caffeic acid, CAPE, galangin and pinocembrin were the main components of the extract. The amounts of caffeic acid and CAPE in the extract were found to be 5.5 and 11.1 mg/g, respectively, by a validated HPLC method. Our study is the first one, revealing effect of propolis on PD‐L1 expression on certain cancer cell lines.

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Section: Protein Expression Analysismentioning

confidence: 99%

Section: Evaluation Of Protein Expression With Flow Cytometrymentioning

confidence: 99%

Antiproliferative Activity of Chemically Characterized Propolis from Turkey and Its Mechanisms of Action

Aru

Güzelmeriç

Akgül

et al. 2019

Chemistry & Biodiversity

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“…Viability (%) of canine osteosarcoma cells treated with five different Colombian propolis samples (Usme, Bogotá Capital District: Usm; Fusagasuga, Cundinamarca: Fus; Puerto Lopez, Meta: Met; Cajibio, Cauca: Caj; and Silvia, Cauca: Sil-50 μg/ml), medium alone (control) and 70% ethanol for 48 h. Assays were repeated four times, and each propolis sample was analysed in quadruplicate (mean ± SD). Different letters indicate statistical differences (p < 0.05) antioxidant properties despite their different compositionKocot, Kiełczykowska, Luchowska-Kocot, Kurzepa, & Musik, 2018) Cao et al (2017). verified that Chinese propolis induced the expression of antioxidant-related genes namely HO-1, GCLM, and GCLC.…”

mentioning

confidence: 76%

“…Propolis from different regions present antioxidant properties despite their different composition (Bankova et al, ; Kocot, Kiełczykowska, Luchowska‐Kocot, Kurzepa, & Musik, ). Cao et al () verified that Chinese propolis induced the expression of antioxidant‐related genes namely HO‐1, GCLM, and GCLC. Other bee products such as pollen also display an antioxidant activity, due to their chemical composition, mainly by the presence of flavonoids (Denisow & Denisow‐Pietrzyk, ).…”

Section: Discussionmentioning

confidence: 99%

The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples

Mora

Santiago

Conti

et al. 2018

Phytotherapy Research

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Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The chemical composition was analyzed by GC–MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC50). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH‐DA. Transwell assay was used to evaluate the invasiveness of propolis‐treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids. Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs.

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“…The main compounds it contains are flavonoids, which have antioxidant characteristics [5]. Propolis has attracted considerable interest in recent years due to its pharmacological and biological effects.…”

Section: Introductionmentioning

confidence: 99%

A Comparison of the Effects of Grayanotoxin-Containing Honey (Mad Honey), Normal Honey, and Propolis on Fracture Healing

et al. 2018

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Objectives: Delayed healing and non-union of fractures have a significant effect upon patient morbidity. Studies have therefore largely concentrated on accelerating fracture healing. This study was intended to compare the effect of “mad honey” and propolis on fracture healing using radiological and histopathological analysis. Subjects and Methods: Femur fracture was surgically performed on 48 rats, followed by fixation. Animals were then divided into 8 groups: 2 control groups (15- and 30-day) and 6 treatment groups (15- and 30-day normal honey, 15- and 30-day “mad honey,” and 15- and 30-day propolis). Rats were sacrificed at the end of these periods, and radiological and histological examinations were performed. Results: Radiological healing in the propolis group after 15-day therapy was statistically better than in the control (p = 0.004) and normal honey (p = 0.006) groups. After 30-day therapy, healing in the propolis group (p = 0.005) and grayanotoxin-containing “mad honey” group (p = 0.007) were significantly better than in the control group. Histologically, there was a statistically significant difference between the 15-day propolis group and the other groups (control, honey, mad honey: p = 0.003, p = 0.003, and p = 0.002, respectively). We also found a statistically significant difference when the 30-day propolis group (p = 0.005) and “mad honey” group (p = 0.007) were compared to the control group. Conclusions: This study shows that grayanotoxin-containing “mad honey” and propolis can accelerate fracture healing.

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Mechanisms underlying the wound healing potential of propolis based on its in vitro antioxidant activity

Cited by 57 publications

References 43 publications

Antiproliferative Activity of Chemically Characterized Propolis from Turkey and Its Mechanisms of Action

Antiproliferative Activity of Chemically Characterized Propolis from Turkey and Its Mechanisms of Action

The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples

A Comparison of the Effects of Grayanotoxin-Containing Honey (Mad Honey), Normal Honey, and Propolis on Fracture Healing

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