Mechanisms Underlying the Regulation of Root Formation in Malus hupehensis Stem Cuttings by Using Exogenous Hormones

Zhang, Wangxiang; Fan, Junjun; Tan, Qian-Qian; Zhao, Mingming; Cao, Fuliang

doi:10.1007/s00344-016-9628-8

Cited by 24 publications

(23 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In order to analyze the synergistic relationship between morphogenesis and the endogenous substances in the rooting process of cuttings, four critical morphological points closely related to rooting were assessed: initial formation of root primordial cells (first critical point), root primordium formation (second critical point), adventitious root breakthrough epidermis (third critical point), and adventitious root massive occurrence (fourth critical point). According to the statistical analysis of the three key enzymes in the study and the data in the literature [ 15 , 24 – 27 ], results showed there were obvious differences between the fit proportion of the four key critical points and the anabolic rhythm of endogenous substances (peak/valley) during rhizogenesis ( Fig 4A ). The endogenous hormones, enzymes, and carbohydrates associated with rooting were mainly fit at the second and third critical points (fit proportion of 30% and 50%, respectively) ( Fig 4B ).…”

Section: Resultsmentioning

confidence: 97%

“…P 1 ’ represents the establishment of primordial cells in the adjacent cambium; P 2 ’ represents adventitious root primordia and meristem initiation; P 3 represents adventitious roots emergence through the epidermis of the stem; P 4 represents the number of root ≥ 3, average root length ≥ 1 cm and the lateral root started to appear. *Adapted from references [ 15 , 24 – 27 ]. Abbreviation: GA 3 , gibberellic acid; IBA, indole butyric acid; ZR, zeatin riboside.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The effects of exogenous hormones on rooting process and the activities of key enzymes of Malus hupehensis stem cuttings

Zhang

Fan

Tan³

et al. 2017

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Malus hupehensis is an excellent Malus rootstock species, known for its strong adverse-resistance and apomixes. In the present study, stem cuttings of M. hupehensis were treated with three types of exogenous hormones, including indole acetic acid (IAA), naphthalene acetic acid (NAA), or green growth regulator (GGR). The effects and mechanisms of exogenous hormone treatment and antioxidant enzyme activity on adventitious root formation were investigated. The results showed that the apparent morphology of the adventitious root had four stages, including root pre-emergence stage (S0), early stage of root formation (S1), massive root formation stage (S2), and later stage of root formation (S3). The suitable concentrations of the three exogenous hormones, IAA, NAA and GGR, were 100 mg·L-1, 300 mg·L-1, and 300 mg·L-1, respectively. They shortened the rooting time by 25–47.4% and increased the rooting percentages of cuttings by 0.9–1.3 times, compared with that in the control. The dispersion in S0 stage was 3.6 times of that in the S1 stage after exogenous hormone application. The earlier the third critical point (P3) appeared, the shorter the rooting time and the greater the rooting percentage of the cuttings. During rhizogenesis, the activities of three antioxidant enzymes (POD, SOD, and PPO) showed an A-shaped trend. However, peak values of enzyme activity appeared at different points, which were 9 d before the P3, P3, and the fourth critical point (P4), respectively. Exogenous hormone treatment reduced the time to reach the peak value by 18 days, although the peak values of the enzymatic activities did not significantly changed. Our results suggested that exogenous hormone treatment mainly acted during the root pre-emergence stage, accelerated the synthesis of antioxidant enzymes, reduced the rooting time, and consequently promoted root formation. The three kinds of antioxidant enzymes acted on different stages of rooting.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

The effects of exogenous hormones on rooting process and the activities of key enzymes of Malus hupehensis stem cuttings

Zhang

Fan

Tan³

et al. 2017

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…This highlights the need for future research on the underlying mechanisms and key factors in the plant-microorganism-abiotic environment continuum. More details about the modes of action of the distinct environmental inputs ( Figure 1 ) on cutting function consider also findings and concepts of Kadner and Druege (2004) , Husen and Pal (2007) , Rapaka et al (2008) , Bredmose and Nielsen (2009) , Agulló-Antón et al (2011) , Osterc and Stampar (2011) , Franken (2012) , da Costa et al (2013) , Bauerfeind et al (2015) , Rasmussen et al (2015) , Otiende et al (2017) , Zhang et al (2017) , Ferrari et al (2018) , Peterson et al (2018) , Taylor and Hoover (2018) , Cho et al (2019) , Heide (2019) , Sanchez et al (2020) and are summarized in Supplementary Table S1 .…”

Section: Systemic Model Of Propagation By Cuttingsmentioning

confidence: 99%

Overcoming Physiological Bottlenecks of Leaf Vitality and Root Development in Cuttings: A Systemic Perspective

Druege

2020

Front. Plant Sci.

View full text Add to dashboard Cite

Each year, billions of ornamental young plants are produced worldwide from cuttings that are harvested from stock plants and planted to form adventitious roots. Depending on the plant genotype, the maturation of the cutting, and the particular environment, which is complex and often involves intermediate storage of cuttings under dark conditions and shipping between different climate regions, induced senescence or abscission of leaves and insufficient root development can impair the success of propagation and the quality of generated young plants. Recent findings on the molecular and physiological control of leaf vitality and adventitious root formation are integrated into a systemic perspective on improved physiologically-based control of cutting propagation. The homeostasis and signal transduction of the wound responsive plant hormones ethylene and jasmonic acid, of auxin, cytokinins and strigolactones, and the carbon-nitrogen source-sink balance in cuttings are considered as important processes that are both, highly responsive to environmental inputs and decisive for the development of cuttings. Important modules and bottlenecks of cutting function are identified. Critical environmental inputs at stock plant and cutting level are highlighted and physiological outputs that can be used as quality attributes to monitor the functional capacity of cuttings and as response parameters to optimize the cutting environment are discussed. Facing the great genetic diversity of ornamental crops, a physiologically targeted approach is proposed to define bottleneck-specific plant groups. Components from the field of machine learning may help to mathematically describe the complex environmental response of specific plant species.

show abstract

“…O processo de formação radicial envolve diversos fatores como condições fisiológicas das plantas matrizes, juvenilidade dos propágulos, balanço hormonal, época de coleta, além da aplicação de reguladores vegetais (Zhang et al 2016). Sabe-se que o material vegetal que contém características juvenis possui maior capacidade de enraizamento que materiais adultos, por apresentar condições fisiológicas, como o balanço hormonal, que favorecem o enraizamento (Hartmann et al 2011;Stuepp et al 2018).…”

Section: Introductionunclassified

Araucaria angustifolia: ácido indol butírico e diferentes clones no enraizamento de estacas

et al. 2020

View full text Add to dashboard Cite

Mechanisms Underlying the Regulation of Root Formation in Malus hupehensis Stem Cuttings by Using Exogenous Hormones

Cited by 24 publications

References 54 publications

The effects of exogenous hormones on rooting process and the activities of key enzymes of Malus hupehensis stem cuttings

The effects of exogenous hormones on rooting process and the activities of key enzymes of Malus hupehensis stem cuttings

Overcoming Physiological Bottlenecks of Leaf Vitality and Root Development in Cuttings: A Systemic Perspective

Araucaria angustifolia: ácido indol butírico e diferentes clones no enraizamento de estacas

Contact Info

Product

Resources

About