Mechanisms underlying atriopeptin-induced increases in hematocrit and vascular permeation in rats.

Williamson, Joseph R.; Holmberg, Sandra; Chang, Katherine; Marvel, Joyce; Sutera, Salvatore P.; Needleman, Philip

doi:10.1161/01.res.64.5.890

Cited by 35 publications

(18 citation statements)

References 22 publications

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“…Previous studies from our laboratory have demonstrated that exogenously administered ANP causes a significant reduction of blood volume and produces transudation of radiolabeled albumin, particularly in fetal skin, but also in muscle , adrenal, bone, kidney, and gut (7). ANPinduced increases in vascular permeation independent of renal effects have been identified in adult animal models as well (21)(22)(23). The correlation between increased ANP concentrations and significantl y expanded extracellular fluid volume in the anemic fetuses is consistent with the hypothesis that ANP may directly or indirectly increase capillary filtration of fluid.…”

Section: Discussionsupporting

confidence: 64%

Role of Endogenous Atrial Natriuretic Peptide in Chronic Anemia in the Ovine Fetus: Effects of a Non-Peptide Antagonist for Atrial Natriuretic Peptide Receptor

Silberbach

et al. 1995

Pediatr Res

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ARSChronic fetal anemia causes polyhydramnios and fetal hydrops and is associated with increased fetal diuresis and natriuresis. To determine the role of atrial natriuretic peptide (ANP) in the renal adaptation to chronic fetal anemia we studied the effects of HS-142-1 (HS), a specific inhibiIor of the guanylate cyclase-linked ANP receptor (ANP -GC), in two groups of chronically instrume nted unanesthetized sheep fetuses. Seven fetuses were made anemic by serial isovolemic hemorrhage over 1 wk, and five fetuses served as nonanemic controls. Over the 7 d of hemorrhage ANP concentrations increased (45 ± 7 to 234 ± 15 fmol/mL). Hematocrit and arterial blood oxygen content were significantly lower in the anemic compared with the nonanemic fetuses (13.8 ± 0.7 versus 34.6 ± 2.3% and 0.7 ± 0.1 versus 2.6 ± 0.2 mrnol/L). Before HS urine flow rate, urinary sodium excretion, fractional excretion of sodium, and renal blood flow we re increased in the anemic fetuses, and the extracellular fluid volume (inulin space) was increased (674 ± 94 versus 497 ± 71 mL/kg). However, GFR was not different between the groups. HS caused a significant increase in the central venous pressure of the anemic fetuses (0.49 ± 0.03 to 0.70 ± 0.05 kPa). Urinary excretion of cGMP was considered to be a marker of endogenous ANP renal effect and was measured before and after a single bolus of HS (5.2 ± 0.30 mg/kg). HS decreased urinary cGMP excretion to 50 and 37% of baseline levels in anemic and nonanemic fetuses, respectively. Urine flow decreased in both nonanemic and anemic fetuses (0.48 ± 0.13 to 0.25 ± 0.06 and 1.30 ± 0.66 ± 0.06 ml.zmin). Sodium excretion decreased in both groups after HS (19 ± 5 to 9 ± 2 and 83 ± 16 to 39 ± 5 p,mol/min). GFR decreased after HS (3.0 ± 0.8 to 2.4 :!:: 0.5 and Alterati ons in feta l kidney function may play an important role in stabilizing the fetus stressed by prolonged anemia. In

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Section: Discussionsupporting

confidence: 64%

Role of Endogenous Atrial Natriuretic Peptide in Chronic Anemia in the Ovine Fetus: Effects of a Non-Peptide Antagonist for Atrial Natriuretic Peptide Receptor

Silberbach

et al. 1995

Pediatr Res

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“…ANP causes significant increases in albumin permeation in adult models (2,12). However, the absolute values of fetal albumin clearance we observed in the control state were 5-to 20-fold higher than those that Williamson cJt 01.…”

Section: Discussioncontrasting

confidence: 59%

“…However, the absolute values of fetal albumin clearance we observed in the control state were 5-to 20-fold higher than those that Williamson cJt 01. (2) described in the adult rat. This finding is consistent with the observation that the capillary filtration coefficient in the fetal sheep is 5-10 times greater than in the adult (13).…”

Section: Discussionmentioning

confidence: 99%

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Effect of Atrial Natriuretic Peptide on Vascular Permeation in the Ovine Fetus

et al. 1994

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ABSTRACT. To study the effect of atrial natriuretic pepIn the adult, the primary action of ANP is to decrease blood tide (ANP) on vascular permeation of albumin in the fetus, volume by increasing urine flow (I). ANP infusion can also lower ANP (167-600 ng/min) was infused into eight ovine fetuses blood volume in anephric experimental animals (2-4), suggesting and saline vehicle was infused into eight twin controls that it causes extravasation of plasma in the adult. Other recent (gestational age 127 f 3 d) over a 50-min period. Using work has implicated ANP in fetal blood volume regulation. ANP two different radiolabeled albumin markers, we determined infusion into the fetus increases hematocrit and decreases blood the tissue to blood isotope ratio (TBIR), an index of volume, even though fetal urine output is only minimally inalbumin permeation, and the albumin clearance. Although creased (5). consistent with this finding is the observation by A N P had no hemodynamic effect, a marked increase in the Robillard cr a/. (6) that ANP causes hemoconcentration even hematocrit was observed in ANP-infused fetuses compared though responsiveness of the fetal kidney to ANP is decreased with initial values (0.37 f 0.04 rs 0.42 f 0.04, p < 0.005) compared with that of the neonatal and adult kidney in animals.but was unchanged in the twin fetuses receiving saline These data raise the possibility that, in the presence of the vehicle (0.35 f 0.03 rersus 0.35 2 0.02). TBIR and albumin functionally immature fetal kidney, the blood volume lowering permeation were increased in combined tissues of AN1'

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“…The right iliac artery cannula was connected to a Statham P50 pressure transducer for monitoring blood pressure as well as for arterial blood sampling, and the left iliac artery cannula was connected to a withdrawal pump for assessment of regional blood flow. The trachea also was Blood flow (expressed as milliliters blood -gram wet weight-' i min-') was measured by injecting 15 Mzm 85Sr-microspheres (NEN Research Products) as described in previous publications (20,21).…”

Section: Skin Chamber Insertionmentioning

confidence: 99%

Diacylglycerol accumulation and microvascular abnormalities induced by elevated glucose levels.

Wolf¹,

Williamson²,

Easom³

et al. 1991

J. Clin. Invest.

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171

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The present experiments were undertaken to examine the hypothesis that glucose-induced increased de novo synthesis of 1,2-diacyl-sn-glycerol (which has been observed in a number of different tissues, including retinal capillary endothelial cells exposed to elevated glucose levels in vitro) and associated activation of protein kinase C may play a role in mediating glucoseinduced vascular functional changes. We report here that twice daily instillation of 30 mM glucose over 10 -d in a rat skin chamber granulation tissue model induces approximately a 2.7-fold increase in diacylglycerol (DAG) levels (versus tissues exposed to 5 mM glucose) in association with marked increases in vascular clearance of albumin and blood flow. The glucose-induced increase in DAG levels as well as the vascular functional changes are prevented by addition of 3 mM pyruvate. Pharmacological activation of protein kinase C with the phorbol ester TPA in the presence of 5 mM glucose increases microvascular albumin clearance and blood flow, and similar effects are observed with 1-monoolein (MOG), a pharmacological inhibitor of the catabolism of endogenous DAG. A pharmacological inhibitor of protein kinase C (staurosporine) greatly attenuates the rise in microvascular albumin clearance (but not the rise in blood flow) induced by glucose or by MOG. These findings are compatible with the hypothesis that elevated concentrations of glucose increase tissue DAG content via de novo synthesis, resuIting in protein kinase C activation, and that these biochemical events are among the factors that generate the increased microvascular albumin clearance. (J. Clin. Invest. 1991.

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Mechanisms underlying atriopeptin-induced increases in hematocrit and vascular permeation in rats.

Cited by 35 publications

References 22 publications

Role of Endogenous Atrial Natriuretic Peptide in Chronic Anemia in the Ovine Fetus: Effects of a Non-Peptide Antagonist for Atrial Natriuretic Peptide Receptor

Role of Endogenous Atrial Natriuretic Peptide in Chronic Anemia in the Ovine Fetus: Effects of a Non-Peptide Antagonist for Atrial Natriuretic Peptide Receptor

Effect of Atrial Natriuretic Peptide on Vascular Permeation in the Ovine Fetus

Diacylglycerol accumulation and microvascular abnormalities induced by elevated glucose levels.

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