Mechanisms Regulating Smooth Muscle Cell Phenotype by Atheroprone and Atheroprotective Hemodynamics on Endothelial Cells

Abstract

Atherosclerosis is an inflammatory disease of the arteries that develops preferentially in regions where shear stress, caused by blood flowing within the lumen of the vessel, is disturbed. Recent evidence demonstrates focal fibronectin (FN) expression in the subendothelial layer in pre-atherosclerotic and advanced lesions. We have found that atheroprone shear stress promotes FN deposition and inflammatory signaling pathways in endothelial cells (ECs). Recently, our lab discovered that EC platelet endothelial cell adhesion molecule-1 (PECAM), which is important for sensing biomechanical stresses, is necessary for the production, secretion, and assembly of the FN-rich matrix in atheroprone regions.Similar to ECs, underlying vascular smooth muscle cells (SMCs) also display a proinflammatory phenotype in regions of atherogenesis, which is a key feature involved in the development of atherosclerosis. Our lab has designed a novel in vitro system that applies human-derived shear stresses to ECs, which are co-cultured with underlying SMCs. This allows for molecular manipulation of individual cell types, while permitting the study of the inherent signaling cross-talk between ECs and SMCs. Such an approach is useful because, while atheroprone shear stress is known to regulate EC signaling and promote a pro-inflammatory environment, the influence of that signaling on SMC phenotype has yet to be investigated.To assess the role of endothelial PECAM and FN signaling in promoting an inflammatory SMC phenotype, the co-culture model was exposed to atheroprone hemodynamic shear stress. siRNA knockdown of either PECAM or FN in ECs reduced SMC inflammatory genes, proteins, and transcription activity. The reduction in SMC inflammation also has the functional consequence of reducing monocyte adhesion. When EC FN is knocked down, the 4 inflammatory SMC phenotype was rescued through the addition of exogenous FN to the EC media. In addition to an increased inflammatory phenotype in response to atheroprone shear stress, SMCs are able to promote inflammation back to ECs. SMC FN was found to regulate EC inflammatory transcription activity and adhesion protein expression.Next, the role of secreted proteins in signaling inflammation from ECs under atheroprone shear stress to SMCs was determined. Cell media collected from EC PECAM and Finally, the atheroprotective role of Kruppel-like factor 4 (KLF4) in the endothelium was investigated. Anti-inflammatory and anti-thrombotic factors were previously shown to be under the regulation of the transcription factor KLF4. To study the role of KLF4 in establishing an atheroprotective phenotype, mono-cultured ECs were exposed to hemodynamic shear stress. KLF4 had increased expression under atheroprotective shear stress compared to atheroprone.Overexpression of KLF4 conferred an atheroprotective EC phenotype even when exposed to an atheroprone shear stress environment. In contrast, knockdown of EC KLF4 resulted in a proinflammatory and pro-thrombotic gene program despite exposure to atheroprotect...