Mechanisms of β-adrenergic receptors agonists in mediating pro and anti-apoptotic pathways in hyperglycemic Müller cells

Safi, Sher Zaman; Saeed, Laiba; Shah, Humaira; Latif, Zahina; Ali, Abid; Imran, Muhammad; Muhammad, Nawshad; Emran, Talha Bin; Subramaniyan, Vetriselvan; Ismail, Izlin

doi:10.1007/s11033-022-07816-0

Cited by 10 publications

(4 citation statements)

References 37 publications

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“…In terms of IC 50 g. ml -1 ), the antiradical activity was expressed. All samples were analyzed in triplicate (Safi et al, 2022;Kayarohanam et al, 2019;Brand-Williams et al, 1995).…”

Section: Determination Of Antioxidant Activitymentioning

confidence: 99%

Methanolic extract of S. securidaca flowers, leaves, and seeds' antihyperlipidemic effects on high fat diet-induced hyperlipidemia in Wistar rats

Aldal’in,

Al-Nadaf,

Awadallah

et al. 2023

Braz. J. Biol.

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Significant risk factors for atherosclerosis include hyperlipidemia and oxidative stress, which together rank as three of the most significant risk factors for cardiovascular diseases. Securigera securidaca lowers cholesterol levels in diabetic rats' blood. This investigation's objective was to determine how methanolic extracts affected the flowers, leaves, and seeds of plants in rats that were fed a high-fat diet (HFD). Five groups of animals were created (n = 5). A total of 35 days, divided into two intervals, were used for the study. Rats received HFD during the first 15-day interval, while during the second 20-day interval, they also received extracts or the Atorvastatin reference drug. The extract of seeds has a high phenol content as well as DPPH radical antioxidant activity. Extracts were given at a dose of 200 mg/kg; p.o. Methanolic treatment of S. securidaca flowers, leaves, and seeds in HFD-induced hyperlipidemic rats resulted in significant reductions in total cholesterol, triglycerides, LDLC, and VLDL-C levels. HDL-C levels increased significantly because of the leaves. While in hyperlipidemic rats, seeds significantly reduced the activities of the enzymes ALT and ALP. The findings showed that, to a certain extent, seeds, flowers, and leaves may have benefits in reducing hyperlipidemia brought on by HFD in terms of lipid profiles and liver function enzymes. The findings of this study indicate a promising application prospect, but more research is needed to determine the exact mechanism of these novel compounds as antihyperlipidemic agents and to clarify their potential combination effect with synthetic drugs such as Atorvastatin. Combinations can reduce the dose of chemical medications required, which lowers the risk of side effects.

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“…In terms of IC 50 g. ml -1 ), the antiradical activity was expressed. All samples were analyzed in triplicate (Safi et al, 2022;Kayarohanam et al, 2019;Brand-Williams et al, 1995).…”

Section: Determination Of Antioxidant Activitymentioning

confidence: 99%

Methanolic extract of S. securidaca flowers, leaves, and seeds' antihyperlipidemic effects on high fat diet-induced hyperlipidemia in Wistar rats

Aldal’in,

Al-Nadaf,

Awadallah

et al. 2023

Braz. J. Biol.

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“…Biotic stress results in almost 10–16% annual crop, along with less yield [ [2] , [3] , [4] , [5] ]. Other than cereal crops, Oil seed crops also have integral role in providing essential edible used for home and different oleochemical industries [ [6] , [7] , [8] , [9] ].…”

Section: Introductionmentioning

confidence: 99%

“…Sesame is also subjected to various stresses, some of them also result in contamination with aflatoxins which make the seeds potential carcinogen. Ecofriendly approach is the need of the time, which can not only mitigate biotic stresses but can also promote growth along with national value of the plant [ 9 , [18] , [19] , [20] ]. Moreover, sesame is underutilized oil seed crop due to various crop establishment and growth issues which render this crop un popular to farmers.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity, metabolic profiling, in-silico molecular docking and ADMET analysis of nano selenium treated sesame seed bioactive compounds as potential novel drug targets against cardiovascular disease related receptors

Ahmad,

Mashwani,

Zohaib Younas

et al. 2024

Heliyon

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“…In response to hyperglycemic environment, several pro-inflammatory pathways also participate during the process of oxidation and antioxidation. Consequently, the imbalance of enzymatic and non-enzymatic antioxidant and ROS generation induce endothelial dysfunction by augmenting endothelium permeability, necrosis and apoptosis in endothelial cells ( Batumalaie et al, 2013 ; SZ Safi et al, 2015 ; Safi et al, 2016 ; Escobar-Morreale et al, 2017 ; Luna and Estévez, 2018 ; Safi et al, 2022 ; Wang et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Hyperglycemia-induced oxidative stress and epigenetic regulation of ET-1 gene in endothelial cells

et al. 2023

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Introduction: Hyperglycemia-induced endothelial dysfunction and the subsequent increase of oxidative stress could lead to aberrant regulation of various genes which are responsible for a range of functions. This study aims to find out how hyperglycemia affect oxidative stress and then the expression and methylation of endothelin 1 (ET-1) gene in in human umbilical vein endothelial cells (HUVEC).Methods: Cells were cultured in growth medium and exposed to low and high glucose concentrations to mimic normal and diabetic condition respectively. Computational analysis were performed using UCSC genome browser and eukaryotic promoter database (EPD). The expression of ET-1 gene was investigated by real time PCR. Cytotoxicity and oxidative stress were determined by MTT and DCFH-DA assays respectively. Promoter methylation was assessed by the bisulfite sequencing method.Results: DCFH-DA assay showed that hyperglycemia can significantly increase the regulation of reactive oxygen species synthesis. The relative expression of ET-1 gene was increased due to exposure to high glucose concentration. MTT assay revealed reduced viability of cells due to the glucose induced damage. Methylation analysis revealed hypomethylation of the promoter of ET-1 however the difference was not significant. Out of 175 CpGs at 25 CpG sites, only 36 CpGs were methylated (20.5% methylation) in cell treated with normal glucose. Upon exposure to high glucose only 30 CpGs were methylated in 175 CpGs at 25 CpG sites (17.1% methylation).Discussion: Our study concludes a significantly high expression of ET-1 gene in response to high glucose exposure in HUVECs. It also reports that hyperglycemic condition leads to elevated oxidative stress. No significant change was found in methylation when cells were treated with high and low glucose concentrations.

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Mechanisms of β-adrenergic receptors agonists in mediating pro and anti-apoptotic pathways in hyperglycemic Müller cells

Cited by 10 publications

References 37 publications

Methanolic extract of S. securidaca flowers, leaves, and seeds' antihyperlipidemic effects on high fat diet-induced hyperlipidemia in Wistar rats

Methanolic extract of S. securidaca flowers, leaves, and seeds' antihyperlipidemic effects on high fat diet-induced hyperlipidemia in Wistar rats

Antioxidant activity, metabolic profiling, in-silico molecular docking and ADMET analysis of nano selenium treated sesame seed bioactive compounds as potential novel drug targets against cardiovascular disease related receptors

Hyperglycemia-induced oxidative stress and epigenetic regulation of ET-1 gene in endothelial cells

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