1999
DOI: 10.1074/jbc.274.45.32099
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Mechanisms of Transcriptional Activation of bcl-2 Gene Expression by 17β-Estradiol in Breast Cancer Cells

Abstract: bcl-2 gene expression is induced by 17␤-estradiol (E2) in T47D and MCF-7 human breast cancer cells, and the mechanism of E2 responsiveness was further investigated by analysis of the bcl-2 gene promoter. The ؊1602 to ؊1534 distal region (bcl-2j) of the promoter was E2-responsive; however, in gel mobility shift assays, the estrogen receptor ␣ (ER ␣ ) did not bind [ 32 P]bcl-2j, whereas Sp1 protein formed a retarded band complex. Further analysis demonstrated that the upstream region (؊1603 to ؊1579) of the bcl-… Show more

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Cited by 239 publications
(192 citation statements)
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“…Recently, regulations of Bcl-2 family proteins by cAMP in the apoptotic cell death were reported. cAMP decreases Bcl-2 in SH-SY5Y cells and enhances apoptotic cell death induced by serum deprivation (26). Contrary to this report, cAMP increases bcl-2 promoter activity in other system through cAMPresponse element (27).…”
Section: Discussioncontrasting
confidence: 90%
“…Recently, regulations of Bcl-2 family proteins by cAMP in the apoptotic cell death were reported. cAMP decreases Bcl-2 in SH-SY5Y cells and enhances apoptotic cell death induced by serum deprivation (26). Contrary to this report, cAMP increases bcl-2 promoter activity in other system through cAMPresponse element (27).…”
Section: Discussioncontrasting
confidence: 90%
“…It has been shown that oestradiol induces Bcl-2 expression at the transcriptional level in MCF-7 cells, and protects the cells from induction of apoptosis (Dong et al, 1999;Mathiasen et al, 1999;Perillo et al, 2000). We therefore tested whether oestradiol could abolish or reduce the growth inhibitory effect of EB1089.…”
Section: Oestradiol Reduces the Growth Inhibitory Response To Eb1089mentioning
confidence: 96%
“…Estrogen has also been shown to trigger T-cell apoptosis by upregulating FAS and FASL in cancer cells. 50 Therefore, the effect of estrogen level on the risk may outweigh that of this FASL rs763110 in estrogen-related cancers. However, more studies are needed to validate the results of this meta-analysis for estrogen-related cancers.…”
Section: Discussionmentioning
confidence: 99%