Mechanisms of transcellular transport of wheat germ agglutinin-functionalized polymeric nanoparticles in Caco-2 cells

Song, Qing; Yao, Lei; Huang, Meng; Hu, Quanyin; Lü, Qing; Wu, Bingxian; Qian, Hong; Rong, Zhengxing; Jiang, Xinguo; Gao, Xiaoling; Chen, Jun; Chen, Hongzhuan

doi:10.1016/j.biomaterials.2012.05.066

Cited by 40 publications

(20 citation statements)

References 55 publications

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“…In addition, the transcellular transport of WGA-functionalized nanoparticles was reported to occur in a cytoskeleton-dependent manner and mainly via a clathrin-mediated mechanism [23]. However, in this study, less WGA than RBA1 was transported across Caco-2 cell monolayers, although WGA showed strong effects on the transport system of the cell monolayers [7].…”

Section: Discussionmentioning

confidence: 76%

Isolation of Rice Bran Lectins and Characterization of Their Unique Behavior in Caco-2 Cells

Nakata

Lin

Abolhassani

et al. 2017

IJMS

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Rice bran lectins, named as RBA1 and RBA2, were isolated from Oryza sativa in two chromatography steps: affinity chromatography and cation-exchange chromatography. RBA1 was found to be composed of a covalently linked heterodimer of 20- and 12-kDa subunits, and RBA2 was a noncovalently linked dimer of 12-kDa subunits. Both RBA1 and RBA2 bound to desialylated complex glycoproteins such as fetuin, α1-acid glycoprotein, and transferrin, and agalactosylated complex glycoproteins such as agalacto fetuin, agalacto-α1-acid glycoprotein, and agalacto-transferrin, in addition to chitooligosacchrides. RBAs were heat stable up to 80 °C and stable at pH 4–10. RBA1 increased the transport of the fluorescent marker, rhodamine 123, which is known to be transported via the P-glycoprotein-mediated efflux pathway across human intestinal Caco-2 cell monolayers. Furthermore, RBA1 itself was transported to the basolateral side of the monolayers via an endocytotic pathway.

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Section: Discussionmentioning

confidence: 76%

Isolation of Rice Bran Lectins and Characterization of Their Unique Behavior in Caco-2 Cells

Nakata

Lin

Abolhassani

et al. 2017

IJMS

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“…Song et al [74] showed that WGA-functionalized PEGylated PLGA NPs (size %120 nm) resulted in superior lysosome escape and enhanced transcellular transport in Caco-2 cells in comparison with NPs with PLA as the core material.…”

Section: Lectinmentioning

confidence: 98%

Advanced Engineering Approaches in the Development of PLGA-Based Nanomedicines

El-Hammadi¹,

Arias²

2015

Handbook of Nanoparticles

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Drug molecules often display little affinity for nonhealthy tissues and/or cells, leading to inefficiency, and high incidence of severe side effects. To face the problem, numerous strategies have been postulated, i.e., chemical modifications to the drug molecule, and proper engineering of drug nanocarriers. In this line, the introduction of poly(D,L-lactide-co-glycolide) nanoparticles in the drug delivery arena has been hypothesized to optimize drug biodistribution and concentration into the targeted site, thus improving the therapeutic effect while reducing the associated drug toxicity. Recent advances in the field have been devoted to the optimization of the in vivo fate and effectiveness of poly(D,L-lactide-co-glycolide)-based drug nanocarriers, i.e., by passive targeting strategies based on the functionalization of the particle surface with special biomolecules, and/or active targeting stratagems thanks to modifications leading to stimuliresponsive nanoparticles. In this chapter, we analyze the current state of the art and future perspectives in the formulation of poly(D,L-lactide-co-glycolide)-based nanomedicines against severe diseases.

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“…Several lectins have been conjugated with PEG-PLGA nanoparticles to promote cellular uptake. 98,99 In most cases, lectins were conjugated to maleimidederivatized PEG-PLGA through a maleimide-thiol coupling reaction. Lectin-functional PEG-PLGA nanoparticles bind to various model cells and become endocytosed by clathrinand caveolae-mediated pathways.…”

Section: Lectin-functional Plga Nanoparticlesmentioning

confidence: 99%

Concepts and practices used to develop functional PLGA-based nanoparticulate systems

Sah¹,

Thoma²,

Desu³

et al. 2013

IJN

194

127

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Abstract:The functionality of bare polylactide-co-glycolide (PLGA) nanoparticles is limited to drug depot or drug solubilization in their hard cores. They have inherent weaknesses as a drug-delivery system. For instance, when administered intravenously, the nanoparticles undergo rapid clearance from systemic circulation before reaching the site of action. Furthermore, plain PLGA nanoparticles cannot distinguish between different cell types. Recent research shows that surface functionalization of nanoparticles and development of new nanoparticulate dosage forms help overcome these delivery challenges and improve in vivo performance. Immense research efforts have propelled the development of diverse functional PLGA-based nanoparticulate delivery systems. Representative examples include PEGylated micelles/nanoparticles (PEG, polyethylene glycol), polyplexes, polymersomes, core-shelltype lipid-PLGA hybrids, cell-PLGA hybrids, receptor-specific ligand-PLGA conjugates, and theranostics. Each PLGA-based nanoparticulate dosage form has specific features that distinguish it from other nanoparticulate systems. This review focuses on fundamental concepts and practices that are used in the development of various functional nanoparticulate dosage forms. We describe how the attributes of these functional nanoparticulate forms might contribute to achievement of desired therapeutic effects that are not attainable using conventional therapies. Functional PLGA-based nanoparticulate systems are expected to deliver chemotherapeutic, diagnostic, and imaging agents in a highly selective and effective manner.

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Mechanisms of transcellular transport of wheat germ agglutinin-functionalized polymeric nanoparticles in Caco-2 cells

Cited by 40 publications

References 55 publications

Isolation of Rice Bran Lectins and Characterization of Their Unique Behavior in Caco-2 Cells

Isolation of Rice Bran Lectins and Characterization of Their Unique Behavior in Caco-2 Cells

Advanced Engineering Approaches in the Development of PLGA-Based Nanomedicines

Concepts and practices used to develop functional PLGA-based nanoparticulate systems

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