Mechanisms of retention of pyrrolidinyl norephedrine on immobilized ?1-acid glycoprotein

Yehl, Peter; O'Brien, Thomas P.; Moeder, Charles; Grinberg, Nelu; Bicker, G.; Wyvratt, Jean

doi:10.1002/(sici)1520-636x(2000)12:3<107::aid-chir2>3.0.co;2-q

Cited by 6 publications

References 32 publications

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Characterization of drug–protein interactions in blood using high‐performance affinity chromatography

Hage

Jackson

Sobansky

et al. 2009

J of Separation Science

144

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The binding of drugs with proteins in blood, serum or plasma is an important process in determining the activity, distribution, rate of excretion, and toxicity of drugs in the body. Highperformance affinity chromatography (HPAC) has received a great deal of interest as a means for studying these interactions. This review examines the various techniques that have been used in HPAC to examine drug-protein binding and discusses the types of information that can be obtained through this approach. A comparison of these techniques with traditional methods for binding studies (e.g., equilibrium dialysis and ultrafiltration) will also be presented. The use of HPAC with specific serum proteins and binding agents will then be discussed, including human serum albumin and α 1 -acid glycoprotein. Several examples from the literature are provided to illustrate the applications of such research. Recent developments in this field are also described, such as the use of improved immobilization techniques, new data analysis methods, techniques for working for directly with complex biological samples, and work with immobilized lipoproteins. The relative advantages and limitations of the methods that are described will be considered and the possible use of these techniques in the high-throughput screening or characterization of drugprotein binding will be discussed.

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Characterization of drug–protein interactions in blood using high‐performance affinity chromatography

Hage

Jackson

Sobansky

et al. 2009

J of Separation Science

144

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New approaches of LC‐MS compatible method development on α₁‐acid glycoprotein‐based stationary phase for resolution of enantiomers by HPLC

Michishita¹,

Franco²,

Zhang³

2010

J of Separation Science

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Protein-based chiral stationary phases, in particular α(1)-acid glycoprotein, are chromatographic materials with a very broad application range. The chiral recognition ability of this selector allows the resolution of more than 90% of the molecules tested. A step-by-step description of the screening approach that we have developed is described in this article with two objectives in mind: obtaining a high success rate with a straightforward methodology that can be generally applied and allowing LC-MS compatibility. Second, the screening strategy is required to take into account the main functional nature of the racemic compound to be resolved (neutral, acidic or basic) for method optimisation step, but should be independent of other structural features, in order to allow the routine application of screening sequences and the application to the largest number of samples.

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Enantiomer-Selective High- and Ultra- High-Performance Liquid Chromatography

Kallenborn

Hühnerfuß

Aboul‐Enein³

et al. 2021

Chiral Environmental Pollutants

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Mechanisms of retention of pyrrolidinyl norephedrine on immobilized ?1-acid glycoprotein

Cited by 6 publications

References 32 publications

Characterization of drug–protein interactions in blood using high‐performance affinity chromatography

Characterization of drug–protein interactions in blood using high‐performance affinity chromatography

New approaches of LC‐MS compatible method development on α₁‐acid glycoprotein‐based stationary phase for resolution of enantiomers by HPLC

Enantiomer-Selective High- and Ultra- High-Performance Liquid Chromatography

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Mechanisms of retention of pyrrolidinyl norephedrine on immobilized ?1-acid glycoprotein

Cited by 6 publications

References 32 publications

Characterization of drug–protein interactions in blood using high‐performance affinity chromatography

Characterization of drug–protein interactions in blood using high‐performance affinity chromatography

New approaches of LC‐MS compatible method development on α1‐acid glycoprotein‐based stationary phase for resolution of enantiomers by HPLC

Enantiomer-Selective High- and Ultra- High-Performance Liquid Chromatography

Contact Info

Product

Resources

About

New approaches of LC‐MS compatible method development on α₁‐acid glycoprotein‐based stationary phase for resolution of enantiomers by HPLC