Mechanisms of Plasma Hemoglobin Clearance after Acute Hemolysis

Andersen, Murray N.; Mouritzen, Christian Y.; Gabrieli, E. R.

doi:10.1097/00000658-196604000-00004

Cited by 20 publications

(5 citation statements)

References 13 publications

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“…In a cardiac arrest population, patients with intra-aortic balloon pumps (IABP) may have on-going low-grade haemolysis. Since the half-life of free haemoglobin is approximately 2–4 hours [ 29 ], considerably shorter than the 30 hour half-life of NSE [ 3 ], an accumulation of NSE relative to free haemoglobin may occur over time. As a consequence, the measured value of NSE may be inappropriately increased beyond the time-point where free haemoglobin is no longer detectable in the sample.…”

Section: Discussionmentioning

confidence: 99%

Serum neuron specific enolase – impact of storage and measuring method

et al. 2014

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BackgroundNeuron specific enolase (NSE) is a recognized biomarker for assessment of neurological outcome after cardiac arrest, but its reliability has been questioned. Our aim was to investigate what influence storage of samples and choice of measuring methods may have on levels of NSE in peripheral blood.MethodsTwo serum samples were drawn simultaneously from 51 hypothermia treated cardiac arrest patients. One sample (original sample) was analysed when collected, using the Diasorin-method (LIAISON®NSE, LNSE). The other sample was frozen, stored at −70°C (stored sample), and reanalysed in the same laboratory 4–7 years later using both the Diasorin method and a Roche-method (NSE Cobas e601, CNSE). In addition, a comparison of the two methods was performed on 29 fresh samples.ResultsThe paired NSE results in original and stored samples were not significantly different, using the LNSE-method. The two methods produced significantly different results (p < 0.0001) on the paired, stored samples, with the CNSE method yielding higher values than the LNSE-method in 96% of samples. The CNSE method resulted in 36% higher values on average. In the method comparison on fresh samples, the CNSE-method generated on average 15% higher values compared to the LNSE-method, and the difference between the paired results was significant (p < 0.0001).ConclusionThe CNSE method generated consistently higher NSE-values than the LNSE method and this difference was more pronounced when frozen samples were analysed. Tolerability for prolonged freezing was acceptable.

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Section: Discussionmentioning

confidence: 99%

Serum neuron specific enolase – impact of storage and measuring method

et al. 2014

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“…The plasma half-life of the haptoglobin-hemoglobin complex in man when studied with tracer amounts of hemoglobin has been estimated to be as short as 10-30 min (18,41), although ti values of 2-4 hr have been reported in studies in which larger amounts of hemoglobin were administered (16,17). On the basis of a few studies in man, it appears that free hemoglobin is cleared even more rapidly than the haptoglobinhemoglobin complex (17,42). Similar findings have been reported in rats (43) and rabbits (44).…”

Section: Discussionmentioning

confidence: 99%

Disposal of plasma heme in normal man and patients with intravascular hemolysis

Sears¹

1970

J. Clin. Invest.

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“…Both these tests were normal. A full blood count, plasma viscosity, blood urea and electrolytes, liver function tests, blood sugar and haptoglobulins (Anderson et al, 1966) were measured on each runner along with urine estimations of free haemoglobin, protein, haemosiderin (Crosby and Dameshek, 1951) and culture. A second sample of blood and urine was taken from each runner at Lancaster and analysed at the Lancaster Royal Infirmary.…”

Section: Measurementsmentioning

confidence: 99%

Haematological and biochemical observations during a 750 mile relay.

Nyman¹

1985

Br J Sports Med

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During a marathon relay from John O'Groats to Lands End (JOGLE) various blood and urine parameters were measured in the runners. Samples of blood and urine were taken from each runner immediately prior to the event and on two occasions during it. Subsequently a three point plot was made for each parameter measured. No special diet was taken. Food and liquid refreshment was provided during the run, the first two days of which came from the organisers. The total distance covered was 870 miles and the time taken one hundred hours and thirty-two minutes. Although the results do not approach statistical significance a trend has been shown suggesting that even with the modern running shoe injury may still occur.

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Mechanisms of Plasma Hemoglobin Clearance after Acute Hemolysis

Cited by 20 publications

References 13 publications

Serum neuron specific enolase – impact of storage and measuring method

Serum neuron specific enolase – impact of storage and measuring method

Disposal of plasma heme in normal man and patients with intravascular hemolysis

Haematological and biochemical observations during a 750 mile relay.

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