Mechanisms of pH-gradient driven transport mediated by organic anion polypeptide transporters

Leuthold, Simone; Hagenbuch, Bruno; Mohebbi, Nilufar; Wagner, Carsten A.; Meier, Peter J.; Stieger, Bruno

doi:10.1152/ajpcell.00436.2008

Cited by 160 publications

(199 citation statements)

References 65 publications

(109 reference statements)

Supporting

Mentioning

176

Contrasting

Unclassified

Order By: Relevance

“…Tc-Mebrofenin Uptake in X. laevis Oocytes X. laevis ooctes were prepared as described before (20). In brief, 30 healthy oocytes were injected with 50nL of water or with 5ng of cRNA encoding human OATP1B1, OATP1B3, OATP2B1(21), or NTCP (22) in 50nL of water.…”

Section: Mmentioning

confidence: 99%

See 1 more Smart Citation

Transporters involved in the hepatic uptake of 99mTc-mebrofenin and indocyanine green

Graaf

Häusler

Heger

et al. 2011

Journal of Hepatology

Self Cite

263

219

View full text Add to dashboard Cite

The transporter specificity of (99m)Tc-mebrofenin and ICG partially overlaps as both compounds are transported by OATP1B3. (99m)Tc-mebrofenin is also taken up by OATP1B1, whereas ICG is additionally transported by NTCP. Accepted ManuscriptTransporters involved in the hepatic uptake of 99m Tc-mebrofenin and indocyanine green This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Section: Mmentioning

confidence: 99%

“…Tc-Mebrofenin and ICG in Stably Transfected CHO Cells Transport experiments were performed in OATP-expressing CHO cells and NTCP-expressing Flp-In CHO cells as described before (17,20). Briefly, cells were washed 3× with pre-warmed (37°C) sodium buffer or choline buffer.…”

Section: Transport Of 99mmentioning

confidence: 99%

Transporters involved in the hepatic uptake of 99mTc-mebrofenin and indocyanine green

Graaf

Häusler

Heger

et al. 2011

Journal of Hepatology

Self Cite

263

219

View full text Add to dashboard Cite

show abstract

“…10), on the other hand, may transport these steroids out of releasing sites. Moreover, since Oatps/OATPs-mediated transport appear to be bidirectional [32], OATP2A1 may also play a role in reuptake of these substances back to the cell and OATP2B1 in delivering blood-borne steroids to target sites.…”

Section: Expression Of the Oatp1a2-and Oatp2b1-immunoreactivity In Thmentioning

confidence: 99%

Differential cellular expression of organic anion transporting peptides OATP1A2 and OATP2B1 in the human retina and brain: implications for carrier-mediated transport of neuropeptides and neurosteriods in the CNS

Gao

Vavricka

Meier

et al. 2014

Pflugers Arch - Eur J Physiol

Self Cite

View full text Add to dashboard Cite

Organic anion transporting polypeptides (OATPs) are polyspecific organic anion transporters, which are expressed in the blood-brain barrier, the choroid plexus, and other organs. The physiologic function of OATPs in extrahepatic tissues remains ambiguous. In rat retina, members of the OATP family are expressed. We therefore investigated the human retina for the expression of OATP1A2 and OATP2B1 and extended the study to human brain. Furthermore, we searched for peptide neurotransmitters as novel OATP substrates. OATP1A2 displayed a broad expression pattern in human retina as assessed by immunofluorescence localization. It is expressed in photoreceptor bodies and somas of amacrine cells. OATP1B2 expression is restricted to the inner nuclear layer and to the inner plexiform layer. Using paraffin sections from human cortex, cerebellum, and hippocampus, OATP1A2 was localized to neurons and neuronal processes, while OATP2B1 is expressed in endothelial cells of brain capillaries. Substance P and vasoactive intestinal peptide were identified as substrates for OATP1A2 and OATP2B1. Doublelabeling immunofluorescence of human retina demonstrated the presence of substance P and of vasoactive intestinal peptides in neurons expressing OATP1A2 and OATP2B1, respectively. The expression of OATP1A2 and OATP2B1 in retinal neurons implies a role of these transporters in the reuptake of peptide neurotransmitters released from retinal neurons. The abundant expression of OATP1A2 in brain neurons points to the possibility that OATP1A2 could be involved in the homeostasis of neurosteroids. The high expression of OATP2B1 in brain capillaries supports an important function of OATPs in substance penetration across the blood-brain barrier. Order of Authors Secondary Information:Abstract: Organic anion transporting polypeptides (OATPs) are polyspecific organic anion transporters, which are expressed in the blood brain barrier, the choroid plexus and other organs. The physiologic function of OATPs in extrahepatic tissues remains ambiguous. In rat retina, members of the OATP family are expressed. We therefore investigated the human retina for the expression of OATP1A2 and OATP2B1 and extend the study to human brain. Furthermore, we searched for peptide neurotransmitters as novel OATP substrates. OATP1A2 displayed a broad expression pattern in human retina as assessed by immunofluorescence localization in fixedfrozen section. It is expressed in photoreceptor bodies and somas of amacrine cells. OATP1B2 expression is restricted to the inner nuclear layer and to the inner plexiform layer. Using paraffin sections from human cortex, cerebellum and hippocampus, OATP1A2 was localized to neurons and neuronal processes, while OATP2B1 is expressed in endothelial cells of brain capillaries. Substance P and vasoactive intestinal peptide were identified as substrates for OATP1A2 and OATP2B1. Doublelabeling immunofluorescence of human retina demonstrated the presence of substance P and of vasoactive intestinal peptides in neurons, which express OAT...

show abstract

“…Recently, evidence was presented that the activity of practically all OATPs is stimulated by a low extracellular pH and that many OATPs act as anion-exchangers with bicarbonate as counter ion (Leuthold et al, 2009). Hence, while anion exchange as basic transport mechanism of OATPs seems well established, clearly more work is needed to obtain detailed insights.…”

Section: Importance Of Transport Mechanismmentioning

confidence: 99%

The emerging role of transport systems in liver function tests

Stieger

Heger

Graaf

et al. 2012

European Journal of Pharmacology

Self Cite

View full text Add to dashboard Cite

Liver function tests are of critical importance for the management of patients with severe or terminal liver disease. They are also used as prognostic tools for planning liver resections. In recent years many transport systems have been identified that also transport substances employed in liver function tests. Such substances include endogenous bilirubin or exogenously administered indocyanine green, agents for magnetic resonance imaging, agents for single photon emission computed tomography or agents for breath tests. The increasing functional and molecular information on the respective transport systems should improve the management and as a result the outcome of patients scheduled for liver surgery or transplantation. To achieve the latter goal, clinical studies that assess individual patients' liver function over the course of their disease with liver function tests are needed to firmly establish and validate recently introduced and novel liver function markers. The emerging role of transport systems in liver function tests Bruno Stieger is supported by grant # 31003A_124652 from the Swiss National Science foundation. AbstractLiver function tests are of critical importance for the management of patients with severe or terminal liver disease. They are also used as prognostic tools for planning liver resections. In recent years many transport systems have been identified, that also transport substances employed in liver function tests. Such substances include endogenous bilirubin or exogenously administered indocyanine green, agents for magnetic resonance imaging, agents for single photon emission computed tomography or agents for breath tests. The increasing functional and molecular information on the respective transport systems should improve the management and as a result the outcome of patients scheduled for liver surgery or transplantation. To achieve the latter goal, clinical studies that assess individual patients liver function over the course of their disease with liver function testes are needed to firmly establish and validate recently introduced and novel liver function markers.

show abstract

Mechanisms of pH-gradient driven transport mediated by organic anion polypeptide transporters

Cited by 160 publications

References 65 publications

Transporters involved in the hepatic uptake of 99mTc-mebrofenin and indocyanine green

Transporters involved in the hepatic uptake of 99mTc-mebrofenin and indocyanine green

Differential cellular expression of organic anion transporting peptides OATP1A2 and OATP2B1 in the human retina and brain: implications for carrier-mediated transport of neuropeptides and neurosteriods in the CNS

The emerging role of transport systems in liver function tests

Contact Info

Product

Resources

About