Mechanisms of Non-Opsonized Zymosan-Induced and Luminol-Enhanced Chemiluminescence in Whole Blood and Isolated Phagocytes

Lindena, J.; Burkhardt, Hannelore; Dwenger, A.

doi:10.1515/cclm.1987.25.11.765

Cited by 37 publications

(46 citation statements)

References 18 publications

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“…The enhanced phagocytosis could be due to increased receptor expression (complement 3b-, Fc-receptor) on the surface of myeloperoxidase deficient granulocytes, which may contribute to the complex pattern of luminol and lucigenin chemiluminescence (29,30). In myeloperoxidase deficient cells, additional numbers of these receptors may be more easily translocated from an intracellular pool to the cell membrane during stimulation (31).…”

Section: Discussionmentioning

confidence: 99%

Phagocytic Activity and Oxidative Burst of Granulocytes in Persons with Myeloperoxidase Deficiency

Gerber¹,

Kuçi²,

Zipfel³

et al. 1996

CCLM

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Summary:In the present study, phagocytosis and the oxidative metabolism of neutrophil granulocytes from five clinically healthy persons with different degrees of myeloperoxidase deficiency were investigated and compared to those of normal persons. The identification of individuals with myeloperoxidase deficiency was performed with the Bayer/Technicon H3 blood cell counter, which differentiates the leukocytes by measuring the peroxidase activity. Neutrophils of three out of five investigated myeloperoxidase deficient persons showed extremly low peroxidase indices (-53 and lower), but only the neutrophils of one person totally lacked myeloperoxidase. This was demonstrated by comparing myeloperoxidase mass concentration measured with an enzyme immunoassay, lack of HOC1 production, and was further confirmed by measuring luminol-and lucigenin-enhanced chemiluminescence. Characteristically, myeloperoxidase deficient granulocytes showed a strikingly decreased luminol-enhanced chemiluminescence while the lucigenin-enhanced chemiluminescence was significantly increased compared to normal granulocytes. Although there is a DNA sequence homology of about 70%, the activity of peroxidase in eosinophils was not affected in any myeloperoxidase deficient person investigated. Moreover, a person with a very rare defect of eosinophil peroxidase had completely normal myeloperoxidase activity. The lack of myeloperoxidase activity is compensated for by an increased phagocytic activity, an increased production of Superoxide anion (lucigeninchemiluminescence) and probably by an alternative metabolism of H 2 O 2 ; since persons lacking myeloperoxidase activity do not normally suffer from severe infections, H 2 O 2 is obviously metabolized to other reactive oxygen substrates than HOC1, e. g. to OH-radicals.

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Section: Discussionmentioning

confidence: 99%

Phagocytic Activity and Oxidative Burst of Granulocytes in Persons with Myeloperoxidase Deficiency

Gerber¹,

Kuçi²,

Zipfel³

et al. 1996

CCLM

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“…We have, however, recently shown that increasing the citrated plasma volume fraction while holding erythrocyte and neutrophil numbers constant results in steadily increasing peak maximum values. In addition, a shift to a higher volume fraction of citrated plasma against a lower volume fraction of erythrocytes has been shown to increase peak maximum counts (18). The decrease in opsonin-dependent (CL1), as well as in opsonin-independent (CL2) peak maximum values from isolated granulocytes does not indicate a more preferential plasma-or cell-mediated alteration.…”

Section: Discussionmentioning

confidence: 96%

“…The major chemiluminescence response of granulocytes to the particulate stimulatory agent, zymosan, is brought about by the interaction of the complement receptor type 3 of granulocytes with the plasma-derived zymosan-coated cleavage products of complement fraction three, iC3b and to a minor degree C3b. In the absence of plasma this receptor also mediates a chemiluminescence response from the interaction with the zymosan polysaccharide component, glucan (18).…”

Section: Chemiluminescence Of Whole Blood and Isolated Granulocytesmentioning

confidence: 99%

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On the Biocompatibility of IBM 2997 Continuous Flow Plateletapheresis

Lindena¹,

Burkhardt²,

Aulmann³

1990

Clinical Chemistry and Laboratory Medicine

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Summary:During the procedure of centrifugation cytapheresis donors occasionally experience adverse clincial reactions. We evaluated the possibility of whether activation of granulocytes and their subsequent release reactions, which may have been triggered by this extracorporeal circuit, were responsible for these adverse effects. Six blood samples were obtained during various set intervals during plateletapheresis. Of these, four samples were taken directly from each donor. The remaining two were drawn from the efferent lines, i.e. those which return blood from the cytapheresis machine back to the donor. Reactive oxygen species produced by granulocytes were monitored by chemiluminescence using microamounts of whole blood or isolated granulocytes. Furthermore, secreted granulocyte products such as neutral proteinase elastase, present in plasma in a complex with oti-proteinase inhibitor (complexed elastase), and lysosomal -glucuronidase were examined. A complete blood cell count, as well as values of haemoglobin, haematocrit, lactate dehydrogenase, protein, albumin and proteinase inhibitors such as oc 2 -macroglobulin and αϊ-proteinase inhibitor were also determined. Complexed elastase increased from a preapheresis value of about 140 μg/l to about 180 μg/l at the end of the cytapheresis. All other clinical chemical and cytological values were 8 to 12 percent lower than preapheresis values, which can be attributed to inherent plasma volume expansion. Reduced chemiluminescence was observed upon stimulation of phagocytes in the whole blood assay (about 700 counts/min χ ΙΟ 3 χ 50000 cells vs. about 600 counts/min χ ΙΟ 3 χ 50000 cells). This decrease was also seen with stimulated granulocytes (about 5800 counts/min χ ΙΟ 3 χ 50000 cells vs. about 4500 counts/min χ ΙΟ 3 χ 50000 cells). Unstimulated granulocytes, on the other hand, showed an increased native chemiluminescence. These data do not, however, indicate a cytapheresis mediated activation of the oxidative metabolism of granulocytes. Concomitant discharge of proteolytic enzymes remains, therefore, of no clinical importance.

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“…Luminol-enhanced CL assay is a very sensitive and reliable tool for determining the opsonic activity of serum (2,(9)(10)(11)16 both serum and blister fluid could be corrected in vitro by adding normal serum to the burn patient's serum (4,6). It was reported that treatment with FFP as adjunctive therapy for massive burns was more effective for maintaining the serum opsonins than that of plasma protein derivative (this product is essentially devoid of IgG and functional complement) (1).…”

mentioning

confidence: 99%

Opsonic Activity of Sera and Blister Fluid from Severely Burned Patients Evaluated by a Chemiluminescence Method

Ono

Kunii

Suzuki

et al. 1994

Microbiology and Immunology

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Burn wound sepsis is the most common and severe complication in the patients with severe burn. To know the systemic and local defect in immunity of burned patients, we measured the luminol-enhanced chemiluminescence (CL) response of normal polymorphonuclear leukocytes (PMNs) upon exposure to zymosan particles, bacteria or Candida albicans that were opsonized with any of patient's serum, blister fluid of burn wound or pooled normal serum (blood type AB). Sera from patients exhibited lower opsonic activities than those of pooled normal serum in the early postburn days. The levels of serum immunoglobulins, complement components and plasma fibronectin were found to correlate well with opsonin-index (OI), which was determined based on the CL response data obtained during the course of infusion therapy with fresh frozen plasma. Furthermore, patient's blister fluid showed much lower opsonic activity against bacteria such as Pseudomonas aeruginosa than patient's own serum. These results indicate that blister fluid is also not effective to opsonize bacteria because of the marked depression of the levels of immunoglobulins and complement components. Destruction of the skin barrier by thermal injury and impairment of systemic or local humoral immunity may predispose these patients to burn wound sepsis.

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Mechanisms of Non-Opsonized Zymosan-Induced and Luminol-Enhanced Chemiluminescence in Whole Blood and Isolated Phagocytes

Cited by 37 publications

References 18 publications

Phagocytic Activity and Oxidative Burst of Granulocytes in Persons with Myeloperoxidase Deficiency

Phagocytic Activity and Oxidative Burst of Granulocytes in Persons with Myeloperoxidase Deficiency

On the Biocompatibility of IBM 2997 Continuous Flow Plateletapheresis

Opsonic Activity of Sera and Blister Fluid from Severely Burned Patients Evaluated by a Chemiluminescence Method

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