Mechanisms of non-disjunction in human female meiosis: the co-existence of two modes of malsegregation evidenced by the karyotyping of 1397 in-vitro unfertilized oocytes

Pellestor, Franck; Andréo, Brigitte; Arnal, Françıoise; Humeau, C.; Demaille, Jacques

doi:10.1093/humrep/17.8.2134

Cited by 112 publications

(104 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…First polar body screening is the only pre-implantation and, more precisely, preconception screening technique allowed by French legislation for recurrent implantation failure, though analysis of the complete cohort, fertilized and unfertilized oocytes, is possible. However, due to time limitations only 76% of first polar bodies could be assessed by FISH, a proportion similar to published values (81.6% [35], 85.5% [4]), but much higher than successful oocyte karyotyping rates (less than 50% of unfertilized oocytes [36]). …”

Section: Discussionsupporting

confidence: 79%

Gamete cytogenetic study in couples with implantation failure: aneuploidy rate is increased in both couple members

Vialard

Hammoud

Molina-Gomès

et al. 2008

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose Implantation failure is known to be associated with an increased risk of aneuploidy in embryos, a situation leading to a pre-implantation genetic screening, not allowed in different countries like France. Our aim was to evaluate the gamete aneuploidy incidence in this context, using first polar body and spermatozoa aneuploidy screening. Methods Three groups were considered: 11 couples with pregnancy obtained after IVF for female infertility (group 1); 20 couples with pregnancy obtained after IVF for male infertility (group 2); and 35 couples with implantation failure (group 3). In group 3, 28 couples treated by ICSI volunteered for first polar body analysis (PB1). Results Spermatozoa aneuploidy rate was increased in groups 2 (1.6%) and 3 (2.1%) in comparison to group 1 (0.6%). PB1 aneuploidy rate was 35.4% in group 3. Finally, eight couples (32%) had no particular chromosomal risk in gametes, 15/25 (60%) presented an increased spermatic (>2%) or oocyte (>1/3) aneuploidy rate, and 2/25 (8%) had both. Conclusion Those results confirm that implantation failure has a heterogeneous origin, that gamete chromosome abnormality rate is one of the major contributing factors, and that 1st Polar body and spermatozoa aneuploidy screening or pre-implantation genetics screening may be indicated for these couples.

show abstract

Section: Discussionsupporting

confidence: 79%

Gamete cytogenetic study in couples with implantation failure: aneuploidy rate is increased in both couple members

Vialard

Hammoud

Molina-Gomès

et al. 2008

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Even if the sample size was too small to allow assessment of aneuploidy after this procedure, no chromosome break was observed in any oocyte group. A large proportion of the oocytes was not interpretable (22/48, 46%) or lost (12/48, 25%), in accordance with a previous study using Tarkowski's method, where the best success rate of karyotyping unfertilised oocytes was 45.9% [45].…”

Section: Discussionsupporting

confidence: 88%

Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?

Hammoud

Molina-Gomès

Albert

et al. 2010

J Assist Reprod Genet

View full text Add to dashboard Cite

Introduction Preconception diagnosis requires first polar body biopsy. When the hole in the zona pellucida is made with a laser beam, heat propagation could, like the biopsy itself, be deleterious. Our aim was to evaluate the effect of this technique on human in vitro matured oocyte and embryo development. Methods One hunded fifty five retrieved immature oocytes from 75 women, matured in vitro, were distributed in 3 groups: 50 oocytes in a control group, without laser drilling and first polar body biopsy, 52 oocytes in a group with only laser drilling, and 53 oocytes in a group with both laser drilling and first polar body biopsy. Safety was evaluated using four criteria: [1] oocyte lysis rate, [2] oocyte activation rate, [3] oocyte development after calcium ionophore treatment, [4] and embryo chromosome breakage incidence after Tarkowski preparation. Results No difference in the four criteria was observed between the 3 oocyte groups.Conclusions We did not find evidence of deleterious effect of laser drilling and first polar body biopsy on in vitro matured oocytes, according to our criteria.

show abstract

“…Current data suggest an aneuploidy rate of 20.8% ( 24,31 and unpublished data), double the rate found by karyotyping ( 22 ). Chromosome loss and gain occurred almost equally in the oocyte and 1 st PB in the abnormal samples, in accordance with the theoretical expectations of the outcome of primary meiotic non-disjunction of the two homologs.…”

Section: The Aneuploidy Rate In Human Oocytessupporting

confidence: 62%

“…Recent karyotyping data that includes chromatid anomalies and the more specific FISH analyses indicated that the overall rate of chromosome and chromatid imbalance in human oocytes is about 11% for women of maternal age 32-34 years ( 15,17,22,35 ). However, several independent studies of aneuploidy in MII oocytes and corresponding 1 st PBs have used CGH to obtain exact information on all chromosomes.…”

Section: The Aneuploidy Rate In Human Oocytesmentioning

confidence: 99%

Errors in Chromosome Segregation During Oogenesis and Early Embryogenesis

Hultén

Smith

Delhanty

2010

Reproductive Endocrinology and Infertility

View full text Add to dashboard Cite

Errors in chromosome segregation occurring during human oogenesis and early embryogenesis are very common. Meiotic chromosome development during oogenesis is subdivided into three distinct phases. The crucial events including meiotic chromosome pairing and recombination takes place from around 11 weeks until birth. Oogenesis is then arrested until ovulation, when the first meiotic division takes place with the second meiotic division not completed until after fertilization. It is generally accepted that most aneuploid fetal conditions, such as for example trisomy 21 Down syndrome, is due to maternal chromosome segregation errors. The underlying reasons are not yet fully understood. It is also clear that superimposed on the maternal meiotic chromosome segregation errors there is a large amount of mitotic errors taking place post-zygotically during the first few cell divisions in the embryo. In this chapter we summarize current knowledge of errors in chromosome segregation during oogenesis and early embryogenesis, with special reference to the clinical implications for successful assisted reproduction.

show abstract

Mechanisms of non-disjunction in human female meiosis: the co-existence of two modes of malsegregation evidenced by the karyotyping of 1397 in-vitro unfertilized oocytes

Cited by 112 publications

References 85 publications

Gamete cytogenetic study in couples with implantation failure: aneuploidy rate is increased in both couple members

Gamete cytogenetic study in couples with implantation failure: aneuploidy rate is increased in both couple members

Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?

Errors in Chromosome Segregation During Oogenesis and Early Embryogenesis

Contact Info

Product

Resources

About