Mechanisms of Neutralization of Endotoxin by Monoclonal IgG Antibodies to Lipopolysaccharide

Sagawa, Teiri; Y, Abe; Kimura, Shigeru; Hitsumoto, Yasuo; Utsumi, S

doi:10.1007/978-3-642-73468-7_61

Cited by 5 publications

(1 citation statement)

References 10 publications

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“…We would like to explain the enhancement of endotoxicity by a disag gregating effect of transferrin. Sagawa et al [14] could demonstrate such an effect for a few monoclonal antibodies which are able to destroy or even enlarge the endotoxin mi celles depending on the epitope of the LPS molecule which is recognized by the anti bodies. In a very hypothetical manner, a similar effect of transferrin is proposed by which the LAL-stimulating portion of the LPS molecule becomes more accessible and the test itself can be stimulated.…”

Section: Discussionmentioning

confidence: 99%

Demonstration of an Interaction between Transferrin and Lipopolysaccharide – An in vitro Study

Berger

Schleich²,

Seidelmann³

et al. 1991

Eur Surg Res

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Transferrin is reported to be a major lipopolysaccharide binding protein of human plasma, at least in vitro. By use of the limulus-amebocyte-lysate test the influence of transferrin on endotoxicity was studied. In the absence of any other protein human iron-free transferrin was able to strongly enhance endotoxicity in a concentration-dependent manner. Similar results were obtained when transferrin was added to primarily heat-inactivated plasma. Even in this assay the endotoxin recovery increased when transferrin was exogenously added. On the other hand, transferrin inhibited endotoxicity when inactivation of the plasma samples was performed after the addition of endotoxin and transferrin. These results lead to the conclusion that transferrin in fact interacts with lipopolysaccharide in a biologically important manner. In order to achieve neutralization of endotoxin, however, other plasma constituents are needed. The hypothetical function of transferrin is possibly a disaggregation of lipopolysaccharide micelles, following the interaction between the two molecules. The present data should justify further studies in order to clarify a possible benefit of the substitution of transferrin during gram-negative sepsis.

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Section: Discussionmentioning

confidence: 99%

Demonstration of an Interaction between Transferrin and Lipopolysaccharide – An in vitro Study

Berger

Schleich²,

Seidelmann³

et al. 1991

Eur Surg Res

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show abstract

Therapy of Secondary T-Cell Immunodeficiencies with Biological Substances and Drugs: An Update

Hadden

1993

Host Defense Dysfunction in Trauma, Shock and Sepsis

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Antiendotoxic Therapy with Polyclonal and Polyvalent Immunoglobulins: In Vitro and In Vivo Studies

Berger

Schleich

Seidelmann

et al. 1993

Host Defense Dysfunction in Trauma, Shock and Sepsis

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Mechanisms of Neutralization of Endotoxin by Monoclonal IgG Antibodies to Lipopolysaccharide

Cited by 5 publications

References 10 publications

Demonstration of an Interaction between Transferrin and Lipopolysaccharide – An in vitro Study

Demonstration of an Interaction between Transferrin and Lipopolysaccharide – An in vitro Study

Therapy of Secondary T-Cell Immunodeficiencies with Biological Substances and Drugs: An Update

Antiendotoxic Therapy with Polyclonal and Polyvalent Immunoglobulins: In Vitro and In Vivo Studies

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Mechanisms of Neutralization of Endotoxin by Monoclonal IgG Antibodies to Lipopolysaccharide

Cited by 5 publications

References 10 publications

Demonstration of an Interaction between Transferrin and Lipopolysaccharide &ndash; An in vitro Study

Demonstration of an Interaction between Transferrin and Lipopolysaccharide &ndash; An in vitro Study

Therapy of Secondary T-Cell Immunodeficiencies with Biological Substances and Drugs: An Update

Antiendotoxic Therapy with Polyclonal and Polyvalent Immunoglobulins: In Vitro and In Vivo Studies

Contact Info

Product

Resources

About

Demonstration of an Interaction between Transferrin and Lipopolysaccharide – An in vitro Study

Demonstration of an Interaction between Transferrin and Lipopolysaccharide – An in vitro Study