Mechanisms of Microbubble-Facilitated Sonoporation for Drug and Gene Delivery

Fan, Zhenzhen; Kumon, Ronald E.; Deng, Cheri X.

doi:10.4155/tde.14.10

Cited by 145 publications

(125 citation statements)

References 135 publications

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“…Sonoporation, for example, involves the use of ultrasound to cause a temporary increase in cell membrane permeability through the creation of pores, allowing the intracellular diffusion of macromolecules such as DNA (Fan et al, 2014). Sonoporation has recently been described for high-efficiency transfection of B cells (Ling Yong et al, 2014) and has been employed to increase in vivo delivery of a DNA vaccines to APCs.…”

Section: Dna Vaccines – Mechanisms Of Action; Increasing Immunogenmentioning

confidence: 99%

DNA vaccines for prostate cancer

Zahm

Colluru

McNeel

2017

Pharmacology & Therapeutics

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DNA vaccines offer many advantages over other anti-tumor vaccine approaches due to their simplicity, ease of manufacturing, and safety. Results from several clinical trials in patients with cancer have demonstrated that DNA vaccines are safe and can elicit immune responses. However, to date few DNA vaccines have progressed beyond phase I clinical trial evaluation. Studies into the mechanism of action of DNA vaccines in terms of antigen-presenting cell types able to directly present or cross-present DNA-encoded antigens, and the activation of innate immune responses due to DNA itself, have suggested opportunities to increase the immunogenicity of these vaccines. In addition, studies into the mechanisms of tumor resistance to anti-tumor vaccination have suggested combination approaches that can increase the antitumor effect of DNA vaccines. This review focuses on these mechanisms of action and mechanisms of resistance using DNA vaccines, and how this information is being used to improve the anti-tumor effect of DNA vaccines. These approaches are then specifically discussed in the context of human prostate cancer, a disease for which DNA vaccines have been and continue to be explored as treatments.

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Section: Dna Vaccines – Mechanisms Of Action; Increasing Immunogenmentioning

confidence: 99%

DNA vaccines for prostate cancer

Zahm

Colluru

McNeel

2017

Pharmacology & Therapeutics

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“…Despite being an active research area for the past 15 years, sonoporation’s exact mechanisms remain relatively misunderstood. 49 More studies are needed to elucidate appropriate parameters for inducing cavitation to achieve effective therapeutic outcomes while maintaining cell function.…”

Section: Perfluorocarbon Particlesmentioning

confidence: 99%

Monitoring/Imaging and Regenerative Agents for Enhancing Tissue Engineering Characterization and Therapies

et al. 2015

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The past three decades have seen numerous advances in tissue engineering and regenerative medicine (TERM) therapies. However, despite the successes there is still much to be done before TERM therapies become commonplace in clinic. One of the main obstacles is the lack of knowledge regarding complex tissue engineering processes. Imaging strategies, in conjunction with exogenous contrast agents, can aid in this endeavor by assessing in vivo therapeutic progress. The ability to uncover real-time treatment progress will help shed light on the complex tissue engineering processes and lead to development of improved, adaptive treatments. More importantly, the utilized exogenous contrast agents can double as therapeutic agents. Proper use of these Monitoring/Imaging and Regenerative Agents (MIRAs) can help increase TERM therapy successes and allow for clinical translation. While other fields have exploited similar particles for combining diagnostics and therapy, MIRA research is still in its beginning stages with much of the current research being focused on imaging or therapeutic applications, separately. Advancing MIRA research will have numerous impacts on achieving clinical translations of TERM therapies. Therefore, it is our goal to highlight current MIRA progress and suggest future research that can lead to effective TERM treatments.

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“…It has been shown that microstreaming shear stress can be exploited in thrombolysis, drug delivery and gene therapy. Microstreaming can rupture and increase the permeability of cell membranes (sonoporation) in biological tissues for better passage of therapeutic agents across the vascular barrier and cell membranes [5][6][7]. The early theory for microstreaming velocity field and the resultant shear stress has been proposed by Nyborg [2].…”

Section: Introductionmentioning

confidence: 99%

Circular Acoustic Microstreaming Flows due to Pulsating Microbubbles near a Membrane

Mobadersany¹,

Sarkar²

2018

Proceedings of the 10th International Symposium on Cavitation (CAV2018)

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In this study, the microstreaming velocity near a plane wall due to microbubble pulsation has been studied. Microbubbles are assumed to pulsate spherically near the wall under the excitation of low amplitude ultrasound. It has been shown an axisymmetric vortex is generated near the wall inducing shear stress on the wall. The shear stress can help facilitate the uptake of drugs and genes in to desired cells through the process called sonoporation.

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Mechanisms of Microbubble-Facilitated Sonoporation for Drug and Gene Delivery

Cited by 145 publications

References 135 publications

DNA vaccines for prostate cancer

DNA vaccines for prostate cancer

Monitoring/Imaging and Regenerative Agents for Enhancing Tissue Engineering Characterization and Therapies

Circular Acoustic Microstreaming Flows due to Pulsating Microbubbles near a Membrane

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