Mechanisms of human plasma protein adsorption on the surface of perfluorocarbon emulsion stabilized with proxanol 268

The effect of apolipoprotein A-I and perfluorocarbon emulsion on the production of specific antibodies in mice

“…In PFCE, the perfluorocarbon (PFC) drops are coated with a layer of triblock copolymer (130PEG-38PPG-130PEG). Our early studies showed that PFCE can adsorb significant amount of proteins which mainly consists of immunoglobulins and various lipoproteins [ 5 6 ]. It is impossible to use this emulsion as an adjuvant directly.…”

“…The second part should be target antigen. We believe that a plausible candidate for the first part is apolipoprotein A-I (ApoAI) which as shown earlier is strongly adsorbed on the surface of the emulsion [ 5 6 ].…”

“…Adsorption process occurs quickly and is fully completed in less than 10 seconds. It is known that upon contact of proteins with fluorocarbon compounds, significant (up to complete) fluorescence quenching occurs [ 6 8 ]. Thus, quenching of intrinsic fluorescence during adsorption indicates the presence of contact between ApoAI_dC-HSP90β and fluorocarbon core of emulsion ( Fig.…”