Mechanisms of Guanylin Action Via Cyclic GMP in the Kidney

Forte, Leonard R.; London, Roslyn M.; Krause, William J.; Freeman, Ronald H.

doi:10.1146/annurev.physiol.62.1.673

Cited by 54 publications

(35 citation statements)

References 73 publications

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“…In mammalian cells, two genes encoding PKG have been identified, type I and type II (40). We found that type I is the major PKG expressed in NRK-49F cells by Western blot analysis (data not shown).…”

Section: Discussionmentioning

confidence: 88%

Effect of Nitric Oxide-cGMP-Dependent Protein Kinase Activation on Advanced Glycation End-Product–Induced Proliferation in Renal Fibroblasts

Huang¹,

Chuang

Guh

et al. 2005

Journal of the American Society of Nephrology

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Section: Discussionmentioning

confidence: 88%

Effect of Nitric Oxide-cGMP-Dependent Protein Kinase Activation on Advanced Glycation End-Product–Induced Proliferation in Renal Fibroblasts

Huang¹,

Chuang

Guh

et al. 2005

Journal of the American Society of Nephrology

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“…Both peptides are produced in the gastrointestinal tract; Ugn primarily by enterochromaffin (EC) cells in the small intestine and Gn by goblet cells in the colon (6,27,28,39,40). Even though both peptides are natriuretic in the kidney, several lines of evidence support a role for Ugn, but not Gn, in volume homeostasis and sodium balance (15)(16)(17)38). For example, in contrast to Gn, Ugn is excreted in urine (11), where it is present at levels that are proportional to dietary sodium intake (18).…”

mentioning

confidence: 99%

Natriuretic and antikaliuretic effects of uroguanylin and prouroguanylin in the rat

Moss

Riguera

Fellner

et al. 2010

American Journal of Physiology-Renal Physiology

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Moss NG, Riguera DA, Fellner RC, Cazzolla C, Goy MF. Natriuretic and antikaliuretic effects of uroguanylin and prouroguanylin in the rat. Am J Physiol Renal Physiol 299: F1433-F1442, 2010. First published September 22, 2010 doi:10.1152/ajprenal.00281.2010.-The peptide uroguanylin (Ugn) is stored and released as a propeptide (proUgn) by enterochromaffin cells in the intestine, and converted to Ugn and other metabolites in the renal tubules. Both proUgn and Ugn are natriuretic, although the response to proUgn is thought to depend on its conversion to Ugn within nephrons. To assess the efficiency of intrarenal conversion of proUgn to Ugn, we measured urinary Ugn excretion in rats following intravenous infusions of proUgn or Ugn. Infusion of 2 and 10 nmol proUgn/kg body wt increased plasma proUgn concentration from 2.2 Ϯ 0.3 to 5.6 Ϯ 1.3 pmol/ml and to 37 Ϯ 9.6 pmol/ml, respectively. No proUgn was detected in urine before, during, or after proUgn infusions. These two proUgn infusion doses resulted in total Ugn recovery in urine of 162 Ϯ 64 and 206 Ϯ 39 pmol/kg body wt (9 and 2% of the infused amount, respectively). By contrast, the same molar amounts of Ugn resulted in 1,009 Ϯ 477 and 5,352 Ϯ 2,133 pmol/kg body wt of Ugn in urine (recoveries of ϳ50%). Unexpectedly, comparisons of natriuretic dose-response curves for each peptide showed proUgn to be about five times more potent than Ugn, despite the relatively modest amount of Ugn generated from infused proUgn. In addition, both peptides were antikaliuretic at low doses, but in this case Ugn showed greater potency than proUgn. These data do not support Ugn as the primary active principle of proUgn for regulation of renal sodium excretion.

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“…It is well established that these two peptides regulate salt and water homeostasis in the kidney (Fonteles et al, 1998) and in the intestine (Forte, 1999), through a pathway that could lead to an endocrine regulation between these two physiological systems. There is evidence of guanylin and uroguanylin receptors within the digestive, renal, cardiac, reproductive, immune-lymphoid organ systems and in the central nervous system (Forte et al, 2000;Fan et al, 1997). In those tissues, these peptides provide a pathway for paracrine and/or autocrine regulation of cellular functions via GMPc.…”

Section: Introductionmentioning

confidence: 99%

Uroguanylin induces electroencephalographic spikes in rats

et al. 2013

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Uroguanylin (UGN) is an endogenous peptide that acts on membrane-bound guanylate cyclase receptors of intestinal and renal cells increasing cGMP production and regulating electrolyte and water epithelial transport. Recent research works demonstrate the expression of this peptide and its receptor in the central nervous system. The current work was undertaken in order to evaluate modifications of electroencephalographic spectra (EEG) in anesthetized Wistar rats, submitted to intracisternal infusion of uroguanylin (0.0125 nmoles/min or 0.04 nmoles/min). The current observations demonstrate that 0.0125 nmoles/min and 0.04 nmoles/min intracisternal infusion of UGN significantly enhances amplitude and frequency of sharp waves and evoked spikes (p = 0.03). No statistical significance was observed on absolute alpha and theta spectra amplitude. The present data suggest that UGN acts on bioelectrogenesis of cortical cells by inducing hypersynchronic firing of neurons. This effect is blocked by nedocromil, suggesting that UGN acts by increasing the activity of chloride channels.Keywords: neuroexcitatory response, brain spikes, neuronal depolarization. Uroguanilina induz potenciais em espiga no eletroencefalograma de ratos ResumoA uroguanilina (UGN) é um peptídeo endógeno que age em receptores do tipo guanilato ciclase de membrana de células intestinais e renais aumentando a produção de GMPc e regulando o transporte epitelial de eletrólitos e água. Pesquisas recentes demonstraram a expressão deste peptídeo e de seus receptores no sistema nervosa central. O presente trabalho foi realizado com objetivo de avaliar possíveis mudanças no espectro do eletroencefalograma (EEG) de ratos Wistar anestesiados, submetidos à infusão intracisternal de uroguanilina (0.0125 nmoles/min or 0.04 nmoles/min). Os resultados apresentados no corrente trabalho demonstram que a infusão intracisternal de ambas as doses de UGN aumenta significativamente a amplitude e frequência das espículas (p = 0.03). Não foram encontradas diferenças estatísticas na amplitude absoluta dos espectros alfa ou teta. Os dados apresentados neste trabalho mostram que a UGN age na bioeletrogênese de células corticais induzindo disparo hipersincrônico de neurônios. Este efeito é bloqueado por nedocromil, sugerindo que UGN atua pelo aumento de atividade de canais de cloreto.Palavras-chave: neuroexcitação, cerebral spikes, despolarização neuronal.

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Mechanisms of Guanylin Action Via Cyclic GMP in the Kidney

Cited by 54 publications

References 73 publications

Effect of Nitric Oxide-cGMP-Dependent Protein Kinase Activation on Advanced Glycation End-Product–Induced Proliferation in Renal Fibroblasts

Effect of Nitric Oxide-cGMP-Dependent Protein Kinase Activation on Advanced Glycation End-Product–Induced Proliferation in Renal Fibroblasts

Natriuretic and antikaliuretic effects of uroguanylin and prouroguanylin in the rat

Uroguanylin induces electroencephalographic spikes in rats

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