Mechanisms of DNA Sensing on Graphene Oxide

Liu, Biwu; Sun, Zhonghui; Zhang, Xu; Liu, Juewen

doi:10.1021/ac401845p

Cited by 208 publications

(202 citation statements)

References 49 publications

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“…Similar observations were made with 13 nm AuNPs capped with A15 ( Figure 2B) and for 5 nm AuNPs capped with A15 ( Figure 2C). While poly-T DNA binds to GO relatively weakly and poly-A binds strongly, [26][27][28] AuNP associated wrinkling was observed in both cases. For comparison, the original citrate capped AuNPs are not adsorbed by GO (Figure S1), and cannot be directly used for this study.…”

mentioning

confidence: 89%

Evaporation induced wrinkling of graphene oxide at the nanoparticle interface

Wang

Liu

2015

Nanoscale

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With the thickness of only a single atomic layer, graphene displays many interesting surface properties. A general observation is that wrinkles are formed on graphene oxide (GO) when it is dried in the presence of adsorbed inorganic nanoparticles. In this case, evaporation induced wrinkling is not an elastic deformation but is permanent. Understanding the nanoscale force of wrinkle formation is important for device fabrication and sensing. Herein, we employ surface functionalized gold nanoparticles (AuNPs) as a model system. All tested AuNPs induced wrinkling, including those capped by DNA, polymers and proteins. The size of AuNPs is less important compared to the property of solvent. Wrinkle formation is attributed to drying related capillary force acting on the GO surface, and a quantitative equation is derived. After drying, the adsorption affinity between GO and AuNPs is increased due to increased contact area.

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confidence: 89%

Evaporation induced wrinkling of graphene oxide at the nanoparticle interface

Wang

Liu

2015

Nanoscale

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“…DNA can be adsorbed by GO and also desorb by adding its cDNA to form a duplex [91]. Ren and Qu and co-workers used DNA-templated AgNCs as fluorophores and extended the DNA templates to include a probe sequence.…”

Section: Molecular Beaconsmentioning

confidence: 99%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

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In the past decade, fluorescent silver, gold and copper nanoclusters (Ag, Au and CuNCs) have emerged as a new class of signaling moiety for biosensor development. Compared to semiconductor quantum dots, metal NCs have less toxicity concerns and can be more easily conjugated to biopolymers. Due to their extremely small size, these NCs need a stabilizing ligand. Many polymers, proteins and nucleic acids have been reported to stabilize NCs. In particular, many DNA sequences produce highly fluorescence NCs. Coupling these DNA stabilizers with other sequences such as aptamers has generated a large number of biosensors. In this review, the synthesis of DNA and nucleotide-templated NCs is first summarized; their chemical interactions are also discussed. In the second part, the properties of NCs such as fluorescence quantum yield, emission wavelength and lifetime, structure and photostability are briefly reviewed. In the last part, various sensor design strategies using these NCs are categorized into the following four classes: 1) fluorescence de-quenching; 2) generation of templating DNA sequences to produce NCs; 3) change of nearby environment; and 4) reacting with heavy metal ions or other quenchers. Finally, the future trends in this field are discussed.

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“…Since under these conditions, the affinity between the aptamer and GO is too strong. 19,50 Finally, the time-dependent sensor signal was followed and the signal was stable over a week ( Figure S8). Response of the freshly prepared and regenerated covalent sensor with 0 or 2 mM ATP.…”

Section: Detection In Buffermentioning

confidence: 99%

“…The binding is strong enough to allow a low background signal, while still weak enough to allow quick probe desorption in the presence of target molecules. [16][17][18][19] For comparison, carbon nanotubes (CNTs) bind DNA much more strongly partially due to DNA wrapping around CNTs, [20][21][22] rendering the release of probe DNA difficult. Finally, DNA recognizes not only complementary nucleic acids, but also many other molecules including metal ions, small molecules, proteins and even whole cells via the aptamer technology.…”

Section: Introductionmentioning

confidence: 99%

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

et al. 2014

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Fluorescent aptamer probes physisorbed on graphene oxide (GO) have recently emerged as a useful sensing platform. Signal is generated by analyte-induced probe desorption. To address non-specific probe displacement and false positive signal, we herein report a covalently linked aptamer probe for ATP detection. A fluorophore and amino dual-modified aptamer was linked to the carboxyl group on GO with a coupling efficiency of ~50%. The linearity, specificity, stability, and regeneration of the covalent sensor was systematically studied and compared to the physisorbed probe. Both sensors have similar sensitivity, but the covalent one is more resistant to non-specific probe displacement by proteins. The covalent sensor has a dynamic range from 0.125 mM to 2 mM ATP in buffer at room temperature and is resistance to DNase I. Intracellular ATP imaging was demonstrated using the covalent sensor, which generated higher fluorescence signal than the physisorbed sensor. After stimulating the cells with 5 mM Ca 2+ for ATP production, the intracellular signal enhanced by 31.8%. This work highlights the advantages of covalent aptamer sensors using GO as both a quencher and a delivery vehicle for intracellular metabolite detection.3

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Mechanisms of DNA Sensing on Graphene Oxide

Cited by 208 publications

References 49 publications

Evaporation induced wrinkling of graphene oxide at the nanoparticle interface

Evaporation induced wrinkling of graphene oxide at the nanoparticle interface

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

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