Mechanisms of Derived Unitegmy among Impatiens Species

McAbee, Jessica Messmer; Kuzoff, Robert K.; Gasser, Charles S.

doi:10.1105/tpc.104.029207

Cited by 49 publications

(72 citation statements)

References 32 publications

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“…Impatiens balsamina has been proposed by McAbee et al (2005) as a suitable species for studying the diversification in the integument morphology within the genus Impatiens. Furthermore, this species has been used as a model for assessing the flowering process and floral reversion (Battley and Lyndon, 1986, 1988Pouteau et al, 1995Pouteau et al, , 1997Pouteau et al, , 1998, all of which support the suitability of this species for a number of studies.…”

Section: Discussionmentioning

confidence: 99%

The reduction in proline buildup in mycorrhizal plants affected by nematodes

Bañuelos

Aguilar

Alarcón

et al. 2012

J. Soil Sci. Plant Nutr.

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Plants stressed by pathogens activate a variety of defense mechanisms to survive. The osmoprotector amino acids, including proline, are among these defense mechanisms. In this work, the effects of arbuscular mycorrhizal fungi on plants infested by root-knot nematodes were evaluated with regard to the accumulation of the osmoprotectant proline. A 2x3 factorial design was established with 8 treatmentswith and without nematodes, with and without mycorrhizae, and with and without fertilizer application -with 4 replicates. Two weeks after inoculation with arbuscular mycorrhizal fungi, the plants were infested with 4 nematode egg masses, and 8 weeks later, the plants were harvested. The inoculation with the arbuscular mycorrhizal fungi significantly reduced the proline content, with the non-inoculated plants exhibiting a higher concentration. Neither the infestation of the nematodes nor the addition of fertilizer significantly affected the proline content. Plant height, stem diameter, leaf area, number of leaves, and fresh weight were significantly improved by the presence of the arbuscular mycorrhizal fungi. The interaction of the fungi and the fertilizer did have a significant effect for height and leaf area. The nematode infestation and the fertilization did not affect mycorrhizal colonization.

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Section: Discussionmentioning

confidence: 99%

The reduction in proline buildup in mycorrhizal plants affected by nematodes

Bañuelos

Aguilar

Alarcón

et al. 2012

J. Soil Sci. Plant Nutr.

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“…Wild-type Arabidopsis stems were used for in situ mRNA localization as described (McAbee et al, 2005). Tissues were fixed in 2.5% formaldehyde and 0.5% glutaraldehyde and then embedded in paraffin.…”

Section: In Situ Localization Of Mrnasmentioning

confidence: 99%

Arabidopsis irregular xylem8andirregular xylem9: Implications for the Complexity of Glucuronoxylan Biosynthesis

et al. 2007

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Mutations of Arabidopsis thaliana IRREGULAR XYLEM8 (IRX8) and IRX9 were previously shown to cause a collapsed xylem phenotype and decreases in xylose and cellulose in cell walls. In this study, we characterized IRX8 and IRX9 and performed chemical and structural analyses of glucuronoxylan (GX) from irx8 and irx9 plants. IRX8 and IRX9 are expressed specifically in cells undergoing secondary wall thickening, and their encoded proteins are targeted to the Golgi, where GX is synthesized. 1 H-NMR spectroscopy showed that the reducing end of Arabidopsis GX contains the glycosyl sequence 4-b-D-, which was previously identified in birch (Betula verrucosa) and spruce (Picea abies) GX. This indicates that the reducing end structure of GXs is evolutionarily conserved in woody and herbaceous plants. This sequence is more abundant in irx9 GX than in the wild type, whereas irx8 and fragile fiber8 (fra8) plants are nearly devoid of it. The number of GX chains increased and the GX chain length decreased in irx9 plants. Conversely, the number of GX chains decreased and the chain length heterodispersity increased in irx8 and fra8 plants. Our results suggest that IRX9 is required for normal GX elongation and indicate roles for IRX8 and FRA8 in the synthesis of the glycosyl sequence at the GX reducing end.

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“…Young wild-type poplar (Populus trichocarpa) stems were fixed in 2.5% formaldehyde and 0.5% glutaraldehyde, embedded in paraffin, and sectioned (12 mm thick) for in situ mRNA localization according to McAbee et al (2005) and Zhou et al (2007). The 200-bp 3# untranslated sequences of poplar transcription factor cDNAs were used for the synthesis of digoxigenin-labeled antisense and sense RNA probes with the DIG RNA Labeling Mix (Roche).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Dissection of the Transcriptional Program Regulating Secondary Wall Biosynthesis during Wood Formation in Poplar

et al. 2011

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Wood biomass is mainly made of secondary cell walls; hence, elucidation of the molecular mechanisms underlying the transcriptional regulation of secondary wall biosynthesis during wood formation will be instrumental to design strategies for genetic improvement of wood biomass. Here, we provide direct evidence demonstrating that the poplar (Populus trichocarpa) wood-associated NAC domain transcription factors (PtrWNDs) are master switches activating a suite of downstream transcription factors, and together, they are involved in the coordinated regulation of secondary wall biosynthesis during wood formation. We show that transgenic poplar plants with dominant repression of PtrWNDs functions exhibit a drastic reduction in secondary wall thickening in woody cells, and those with PtrWND overexpression result in ectopic deposition of secondary walls. Analysis of PtrWND2B overexpressors revealed up-regulation of the expression of a number of wood-associated transcription factors, the promoters of which were also activated by PtrWND6B and the Eucalyptus EgWND1. Transactivation analysis and electrophoretic mobility shift assay demonstrated that PtrWNDs and EgWND1 activated gene expression through direct binding to the secondary wall NAC-binding elements, which are present in the promoters of several wood-associated transcription factors and a number of genes involved in secondary wall biosynthesis and modification. The WND-regulated transcription factors PtrNAC150, PtrNAC156, PtrNAC157, PtrMYB18, PtrMYB74, PtrMYB75, PtrMYB121, PtrMYB128, PtrZF1, and PtrGATA8 were able to activate the promoter activities of the biosynthetic genes for all three major wood components. Our study has uncovered that the WND master switches together with a battery of their downstream transcription factors form a transcriptional network controlling secondary wall biosynthesis during wood formation.Wood, composed of cellulose, hemicelluloses, and lignin, is the most abundant biomass produced by plants. It is an important raw forest product that has traditionally been used for myriad applications, including construction, pulping, paper making, direct burning for energy, and so on. Recently, wood from tree species, such as poplar (Populus spp.), has been proposed to be a renewable source for biofuel production (Carroll and Somerville, 2009). Therefore, there is a surge of interest in elucidating the molecular mechanisms underlying the process of wood formation in the hope of developing strategies for increasing wood biomass production and/or modifying wood composition tailored for biofuel production. Since wood biomass is mainly made of secondary walls, elucidation of the mechanisms underlying the coordinated activation of secondary wall biosynthetic genes will undoubtedly contribute to our understanding of the molecular control of wood formation.Molecular and genomic studies in tree species have uncovered a number of wood-associated transcription factors that might be involved in the coordinated regulation of wood formation (Patzlaff et al

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Mechanisms of Derived Unitegmy among Impatiens Species

Cited by 49 publications

References 32 publications

The reduction in proline buildup in mycorrhizal plants affected by nematodes

The reduction in proline buildup in mycorrhizal plants affected by nematodes

Arabidopsis irregular xylem8andirregular xylem9: Implications for the Complexity of Glucuronoxylan Biosynthesis

Dissection of the Transcriptional Program Regulating Secondary Wall Biosynthesis during Wood Formation in Poplar

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