Mechanisms of Biotherapy Effect of Shenfoweikang Herbs on Gastric Carcinoma Cells

Abstract

To explore the biotherapy effect of Shenfoweikang herbs in treatment of gastric cancer, BALB/C mice were grafted with a mouse gastric adenocarcinoma cell line MFC as the experimental model. The mice were divided into four groups. Mice in the experimental groups received different doses of Shenfoweikang herbs over a 60-day period starting at the first day after grafting. Mice received saline as controls. All the mice were sacrificed at 61 days after being grafted. Tumor size was periodically measured during the life of the mice and tumor weight was determined by an electron balance immediately after the animals killed. Serum cytokines, granzyme B and perforin were examined by the ELISA method. The anti-tumor effect was detected by the cytotoxic T-lymphocyte (CTL) assays. Our results demonstrated that Shenfoweikang herbs could inhibit the growth of gastric cancer by activating the CTLs and inducing the secretion of cytokines, perforin and granzyme B. Our study suggests that Shenfoweikang herbs inhibited the proliferation of gastric carcinoma by activating the effects of immune cells, which may lay a better basis for further study on gastric cancer biotherapy.