Mechanisms of astrocyte development

β-catenin signaling via astrocyte-encoded TCF7L2 regulates neuronal excitability and social behavior

“…including Wnt/β-catenin signaling, coordinate astrocyte specification 9 , the roles of β-catenin cascade in the regulation of astrocyte maturation are not well understood.…”

“…During that period astrocytes increase in volume and process complexity, and start expressing markers of functional maturity including gap junction connexins and potassium channels 78 . While multiple developmental pathways, including Wnt/β-catenin signaling, coordinate astrocyte specification 9 , the roles of β-catenin cascade in the regulation of astrocyte maturation are not well understood.…”

Time series scRNAseq analysis in mouse and human informs optimization of rapid astrocyte differentiation protocols

“…Using our scRNAseq data we optimized previous mouse astrocyte diff erentiation protocols by highlighting and removing non-required transition states and decreasing the overall protocol from 3 weeks to less than 12 days. Our data will be useful for researchers interested in optimizing glial diff erentiations in either species, and provide a window into human glial diff erentiation, which is diffi cult to study given its lateness in development.trolled by a combination of various transcription factors including Nfi a 16,17 , Nfi b 18,19 , Sox9 [20][21][22] , and signaling pathways like Notch 23,24 (reviewed elsewhere [25][26][27][28] ). Studies have begun to uncover regional specifi cations to this process [29][30][31] in line with a recent focus on identifying brain-area specifi c astrocyte subtypes [32][33][34][35] .In humans, much less is understood about the mechanisms directing gliogenesis 27,36 , and neurogenesis more broadly.…”

“…Our data will be useful for researchers interested in optimizing glial diff erentiations in either species, and provide a window into human glial diff erentiation, which is diffi cult to study given its lateness in development.trolled by a combination of various transcription factors including Nfi a 16,17 , Nfi b 18,19 , Sox9 [20][21][22] , and signaling pathways like Notch 23,24 (reviewed elsewhere [25][26][27][28] ). Studies have begun to uncover regional specifi cations to this process [29][30][31] in line with a recent focus on identifying brain-area specifi c astrocyte subtypes [32][33][34][35] .In humans, much less is understood about the mechanisms directing gliogenesis 27,36 , and neurogenesis more broadly. Even though we understand less about human glial development, there are protocols for human astrocyte diff erentiation that have been verifi ed at the scRNAseq level by comparing gene expression to acutely isolated human astrocytes 37 .…”

“…trolled by a combination of various transcription factors including Nfi a 16,17 , Nfi b 18,19 , Sox9 [20][21][22] , and signaling pathways like Notch 23,24 (reviewed elsewhere [25][26][27][28] ). Studies have begun to uncover regional specifi cations to this process [29][30][31] in line with a recent focus on identifying brain-area specifi c astrocyte subtypes [32][33][34][35] .…”