Mechanisms of Asbestos-induced Nitric Oxide Production by Rat Alveolar Macrophages in Inhalation and in vitro Models 11Supported by grants from NHLBI (RO1 HL39469) and NIEHS (RO1 03878).

Quinlan, Timothy R.; Bérubé, Kelly Ann; Hacker, Miles P.; Taatjes, Douglas J.; Timblin, Cynthia R.; Goldberg, Jonathan; Kimberley, Priscilla; O’Shaughnessy, Patrick T.; Hemenway, David R.; Torino, Jennifer; Jiménez, Luis A.; Mossman, Brooke T.

doi:10.1016/s0891-5849(97)00357-2

Cited by 73 publications

(45 citation statements)

References 31 publications

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“…The roles of inflammatory responses and nitrative stress in asbestos-related diseases have been examined. iNOS expression and RNS generation have been observed in asbestostreated cultured human alveolar macrophages 20) , lung epithelial cells 21) and mesothelial cells 22) , and in the bronchiolar epithelium of asbestos-exposed animals 23,24) . In clinical studies, the alveolar NO concentration was increased in asbestos-exposed subjects compared with control subjects 25) .…”

Section: Discussionmentioning

confidence: 99%

Formation of the Nitrative DNA Lesion 8‐Nitroguanine is Associated with Asbestos Contents in Human Lung Tissues: A Pilot Study

Hiraku

Saito²,

Shibata

et al. 2014

Journal of Occupational Health

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Formation of the Nitrative DNA Lesion 8‐Nitroguanine is Associated with Asbestos Contents in Human Lung Tissues: A Pilot Study: Yusuke HIRAKU, et al. Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine— Objectives Asbestos causes lung cancer and malignant mesothelioma, and chronic inflammation is considered to participate in carcinogenesis. However, biomarkers to evaluate its carcinogenic risk have not been established. Reactive oxygen/nitrogen species are generated in biological systems under inflammatory conditions and may contribute to carcinogenesis by causing DNA damage. In this study, we examined the relationship between the formation of 8nitroguanine (8‐nitroG), a mutagenic DNA lesion formed during inflammation, and asbestos contents in human lung tissues. Methods We obtained non‐tumor lung tissues from patients with (n=15) and without mesothelioma (n=21). The expression of 8‐nitroG and related molecules was examined by immunohistochemistry, and their staining intensities were semiquantitatively evaluated. Asbestos contents in lung tissues were analyzed by analytical transmission electron microscopy. Results In subjects without mesothelioma, staining intensities of 8‐nitroG and apurinic/apyrimidinic endonuclease 1 (APE1) were significantly correlated with total asbestos and amphibole contents (p<0.05), but not with chrysotile content. In mesothelioma patients, their staining intensities were not correlated with asbestos contents. The double immunofluorescence technique revealed that APE1 was expressed in 8‐nitroGpositive cells, suggesting that abasic sites were formed possibly due to the removal of 8‐nitroG. The staining intensities of 8‐oxo‐7,8‐dihydro‐2’‐deoxyguanosine, an oxidative DNA lesion, and its repair enzyme 8‐oxoguanine DNA‐glycosylase were correlated with age (p<0.05), but not with asbestos contents in subjects without mesothelioma. Conclusions This is the first study to demonstrate that 8‐nitroG formation is associated with asbestos contents in human lung tissues. This finding raises a possibility that 8‐nitroG serves as a biomarker that can be used to evaluate asbestos exposure and carcinogenic risk.

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Section: Discussionmentioning

confidence: 99%

Formation of the Nitrative DNA Lesion 8‐Nitroguanine is Associated with Asbestos Contents in Human Lung Tissues: A Pilot Study

Hiraku

Saito²,

Shibata

et al. 2014

Journal of Occupational Health

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“…In light of this, an effort is now being made to develop a specific NOS inhibitor effective in macrophages. [11][12][13] We have previously reported that the aqueous extract of Tinospora tuberculata inhibited NO production in macrophages in a dose-dependent manner. 3) The effects of Tinospora tuberculata were also evident in a cell-free system; Tinospora tuberculata inhibited the production of NO from L-Arg and hydrogen peroxide, and dose-dependently suppressed the release of NO donors from sodium nitroprusside.…”

Section: Discussionmentioning

confidence: 99%

Examination of the Nitric Oxide Production-Suppressing Component in Tinospora tuberculata.

Yokozawa

Tanaka

Kimura

2001

Biological & Pharmaceutical Bulletin

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“…The NO synthesis is mainly regulated by a variety of mechanisms at the transcrip tional and posttranslational levels by inducible NOS (iNOS) in activated macrophages. In the light of this evi dence, efforts are now being made to develop an agent that specifically inhibits NOS in macrophages (16)(17)(18). LPS, a Gram-negative bacterial outer membrane component, has been identified as one of the critical fac tors involved in the pathogenesis of sepsis (19).…”

Section: Methodsmentioning

confidence: 99%

Mustard Leaf Suppresses Nitric Oxide Synthesis by Mouse Macrophages

Kim

Yokozawa

Cho

2005

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThe effect of mustard leaf on nitric oxide (NO) production was investigated using lipopolysaccharide (LPS)-stimulated peritoneal macrophages . LPS induced the pro duction of a large amount of nitrite, an indicator of NO . However, the addition of the ethyl acetate (EtOAc) or n-butanol (BuOH) fraction of mustard leaf to LPS-stimulated peritoneal macrophages inhibited excessive production of nitrite significantly . Moreover, compared with no treatment, LPS impaired cell viability significantly . However, the EtOAc fraction of mustard leaf significantly protected cells that had been exposed to LPS . In addition, the result of nitrite production per cell indicated that the mustard leaf fractions significantly suppressed nitrite synthesis by macrophages. In particular , the EtOAc fraction was a stron ger inhibitor of nitrite synthesis than the BuOH fraction. As NO is one of the critical media tors in various disorders, these results may help to explain certain pharmacological activities of mustard leaf.

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Mechanisms of Asbestos-induced Nitric Oxide Production by Rat Alveolar Macrophages in Inhalation and in vitro Models 11Supported by grants from NHLBI (RO1 HL39469) and NIEHS (RO1 03878).

Cited by 73 publications

References 31 publications

Formation of the Nitrative DNA Lesion 8‐Nitroguanine is Associated with Asbestos Contents in Human Lung Tissues: A Pilot Study

Formation of the Nitrative DNA Lesion 8‐Nitroguanine is Associated with Asbestos Contents in Human Lung Tissues: A Pilot Study

Examination of the Nitric Oxide Production-Suppressing Component in Tinospora tuberculata.

Mustard Leaf Suppresses Nitric Oxide Synthesis by Mouse Macrophages

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