Mechanisms of arm regeneration in the feather star <i>Antedon mediterranea</i>: Healing of wound and early stages of development

Carnevali, M. Daniela Candia; Lucca, Elisa; Bonasoro, F.

doi:10.1002/jez.1402670308

Cited by 53 publications

(83 citation statements)

References 12 publications

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“…LM stained sections were observed and photographed with a Jenaval light microscope. TEM sections were observed and photographed in a Jeol 100SX electron microscope (for details see [13]). …”

Section: Methodsmentioning

confidence: 99%

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Visceral regeneration in the crinoid Antedon mediterranea: basic mechanisms, tissues and cells involved in gut regrowth

et al. 2006

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Abstract:Crinoids are able to regenerate completely many body parts, namely arms, pinnules, cirri, and also viscera, including the whole gut, lost after self-induced or traumatic mutilations. In contrast to the regenerative processes related to external appendages, those related to internal organs have been poorly investigated. In order to provide a comprehensive view of these processes, and of their main events, timing and mechanisms, the present work is exploring visceral regeneration in the feather star Antedon meditteranea. The histological and cellular aspects of visceral regeneration were monitored at predetermined times (from 24 hours to 3 weeks post evisceration) using microscopy and immunocytochemistry. The overall regeneration process can be divided into three main phases, leading in 3 weeks to the reconstruction of a complete functional gut. After a brief wound healing phase, new tissues and organs develop as a result of extensive cell migration and transdifferentiation. The cells involved in these processes are mainly coelothelial cells, which after trans-differentiating into progenitor cells form clusters of enterocytic precursors. The advanced phase is then characterized by the growth and differentiation of the gut rudiment. In general, our results confirm the striking potential for repair (wound healing) and regeneration displayed by crinoids at the organ, tissue and cellular levels.

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Section: Methodsmentioning

confidence: 99%

“…They are able to completely regenerate arms [2,[11][12][13][14][15][16][17][18][19][20][21], pinnules, cirri, and also viscera, including the whole gut, after self-induced or traumatic mutilation [1,2].…”

Section: Introductionmentioning

confidence: 99%

Visceral regeneration in the crinoid Antedon mediterranea: basic mechanisms, tissues and cells involved in gut regrowth

et al. 2006

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“…Regeneration was induced following the natural strategies as far as possible, i.e. amputating the arm at the level of the autotomy plane (for details see Candia Carnevali et al 1993). Three arms were removed from each experimental animal immediately before introducing the specimens in the respective aquaria.…”

Section: Methodsmentioning

confidence: 99%

“…The regenerating arms sampled were analysed by light microscope (SM, stereomicroscope, and LM, standard light microscope) and electron microscope (TEM, trasmission electron microscope, and SEM, scanning electron microscope) as described in detail in previous papers (see Candia Carnevali et al 1993). Additional methods were used for monitoring cell proliferation, employing 5-bromodeoxyuridine (BrdU) and related immunocytochemistry (ICC) protocols (Candia Carnevali et al 1995, 1997, and other ICC methods were used for detecting specific molecules.…”

Section: Methodsmentioning

confidence: 99%

“…It is characterised by a frequent and spontaneous arm autotomy followed by prompt and complete regeneration of the arm (Reichensperger 1912, Clark 1921. Arm regeneration in A. mediterranea involves an epimorphic blastemal process which has been extensively investigated and reconstructed in detail, particularly with respect to its cellular and molecular aspects (Candia Carnevali et al 1993, 1997. For this reason it provides an ideal experimental model to test tissue and cellular dysfunctions related to regenerative development and growth .…”

Section: Introductionmentioning

confidence: 99%

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PCB exposure and regeneration in crinoids (Echinodermata)

Carnevali¹,

Bonasoro²,

Patruno³

et al. 2001

Mar. Ecol. Prog. Ser.

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Echinoderm regeneration provides a convenient and tractable test to monitor the effects of persistent micropollutants on the developmental physiology of marine animals. Regeneration involves crucial and sensitive biological processes (cell proliferation, morphogenesis, differentiation, tissue renewal) that make it an ideal tool to assess micropollutants at the tissue and cellular level. Our results provide evidence that the regenerative response in the crinoid Antedon mediterranea is especially sensitive to PCBs and that exposure to these chemicals induces significant variations in timing and modality of arm regeneration. Prolonged exposure to low concentrations of Aroclor ® 1260 (14 ng l -1 of total PCBs), typical of moderately polluted coastal zones, results in abnormal arm growth in terms of both gross morphology and microscopic anatomy. In terms of tissue/cellular aspects the main modifications are: accelerated growth of the regenerate; massive cell migration/proliferation; hypertrophic development of the coelomic canals; and, extensive rearrangement of differentiated tissues of the stump. The anomalies observed in the developmental regenerative processes appear to be compatible with a pattern of pseudo-endocrine activities.

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Regeneration of the enteric nervous system in the sea cucumberHolothuria glaberrima

et al. 1999

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Among higher metazoans, echinoderms exhibit the most impressive capacity for regeneration. Holothurians, or sea cucumbers, respond to adverse stimuli by autotomizing and ejecting their visceral organs, which are then regenerated. Neuronal fibers and cell bodies are present within the viscera, but previous regeneration studies have not accounted for the nervous component. We used light microscopic immunocytochemistry and ultrastructural studies to describe the regeneration of the enteric nervous system in the sea cucumber Holothuria glaberrima. This study provides evidence that the enteric nervous system of this echinoderm regenerates after evisceration and that in 3–5 weeks the regenerated system is virtually identical to that of noneviscerated animals. The regeneration of the enteric nervous system occurs parallel to the regeneration of other organ components. Nerve fibers and cells are observed within the mesenterial thickenings that give rise to the new intestine and within the internal connective tissue prior to lumen formation. We also used bromodeoxyuridine incorporation to show that proliferation of the neuronal population occurs in the regenerating intestine. The regeneration of the nervous system commands high interest because members of the closely related phylum Chordata either lack or have a very limited capacity to regenerate their nervous system. Thus, holothurians provide a model system to study enteric nervous system regeneration in deuterostomes. J. Comp. Neurol. 406:461–475, 1999. © 1999 Wiley‐Liss, Inc.

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Mechanisms of arm regeneration in the feather star Antedon mediterranea: Healing of wound and early stages of development

Cited by 53 publications

References 12 publications

Visceral regeneration in the crinoid Antedon mediterranea: basic mechanisms, tissues and cells involved in gut regrowth

Visceral regeneration in the crinoid Antedon mediterranea: basic mechanisms, tissues and cells involved in gut regrowth

PCB exposure and regeneration in crinoids (Echinodermata)

Regeneration of the enteric nervous system in the sea cucumberHolothuria glaberrima

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