Mechanisms of antibiotic resistance to enrofloxacin in uropathogenic Escherichia coli in dog

Piras, Cristian; Soggiu, Alessio; Greco, Viviana; Martino, Piera Anna; Chierico, Federica Del; Putignani, Lorenza; Urbani, Andrea; Nally, Jarlath E.; Bonizzi, L.; Roncada, Paola

doi:10.1016/j.jprot.2015.05.040

Cited by 36 publications

(41 citation statements)

References 76 publications

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“…The Peptide Mass Fingerprinting Analysis was performed according to Piras et al 30 Briefly, the MS analysis was performed on an Ultraflex III MALDI-TOF/TOF spectrometer (BrukerDaltonics) in positive reflectron mode and MS spectra were analyzed by FlexAnalysis 3.3 software (Bruker-Daltonics) to select monoisotopic peptide masses. …”

Section: Maldi Tof Tof Ms Analysismentioning

confidence: 99%

“…Label-free nanoflow liquid chromatography mass spectrometry (nLC-MS E ) was performed as previously described in [30][31][32] . Briefly, the protein extracts of nine biological samples (3 dosages LPS from cell culture from 3 cows) were precipitated with a cold mix of Ethanol, Methanol, and Acetone (ratio 2:1:1, v/v), then dissolved in 6 M Urea, 100 mM Tris pH 7.5 and digested 50:1 (w⁄w) with sequence grade trypsin (Promega, Madison, WI, USA) at 37°C overnight after reduction with 10 mM DTT and alkylation with 20 mM IAA.…”

Section: Expression Analysis By Nlc-msmentioning

confidence: 99%

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Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E. coli LPS challenge

et al. 2017

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Section: Maldi Tof Tof Ms Analysismentioning

confidence: 99%

Section: Expression Analysis By Nlc-msmentioning

confidence: 99%

Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E. coli LPS challenge

et al. 2017

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“…Quorum sensing can regulate multidrug resistance by upregulation of biofilm-associated genes and efflux pump genes [64,65]. Using an integrated proteomics study, Piras et al [66,67] identified many quorum sensing-related proteins (e.g., LuxS) differentially expressed in multi-drug resistant E. coli compared to a control E. coli group, suggesting the association between quorum sensing and drug resistance. In A. baumannii , the quorum sensing system has also been identified and proved to play a key role in the regulation of bacterial virulence and biofilm formation [68].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Determinant-Focused Acinetobacter baumannii Vaccine Designed Using Reverse Vaccinology

Chen

Ong

et al. 2017

IJMS

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As one of the most influential and troublesome human pathogens, Acinetobacter baumannii (A. baumannii) has emerged with many multidrug-resistant strains. After collecting 33 complete A. baumannii genomes and 84 representative antibiotic resistance determinants, we used the Vaxign reverse vaccinology approach to predict classical type vaccine candidates against A. baumannii infections and new type vaccine candidates against antibiotic resistance. Our genome analysis identified 35 outer membrane or extracellular adhesins that are conserved among all 33 genomes, have no human protein homology, and have less than 2 transmembrane helices. These 35 antigens include 11 TonB dependent receptors, 8 porins, 7 efflux pump proteins, and 2 fimbrial proteins (FilF and CAM87009.1). CAM86003.1 was predicted to be an adhesin outer membrane protein absent from 3 antibiotic-sensitive strains and conserved in 21 antibiotic-resistant strains. Feasible anti-resistance vaccine candidates also include one extracellular protein (QnrA), 3 RND type outer membrane efflux pump proteins, and 3 CTX-M type β-lactamases. Among 39 β-lactamases, A. baumannii CTX-M-2, -5, and -43 enzymes are predicted as adhesins and better vaccine candidates than other β-lactamases to induce preventive immunity and enhance antibiotic treatments. This report represents the first reverse vaccinology study to systematically predict vaccine antigen candidates against antibiotic resistance for a microbial pathogen.

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“…faecalis in the gastrointestinal tract, will be spared should be taken into account [118]. Therefore, transcriptomic and proteomic analyses of quorum-sensing inhibitors seems an elegant way to determine the mechanisms used by different inhibitors with respect to virulence and biofilm formation in the pathogenic bacteria [119,120,121]. …”

Section: Perspectivesmentioning

confidence: 99%

Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches

Ali

Goraya

Ajmal

et al. 2017

IJMS

118

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Quorum-sensing systems control major virulence determinants in Enterococcus faecalis, which causes nosocomial infections. The E. faecalis quorum-sensing systems include several virulence factors that are regulated by the cytolysin operon, which encodes the cytolysin toxin. In addition, the E. faecalis Fsr regulator system controls the expression of gelatinase, serine protease, and enterocin O16. The cytolysin and Fsr virulence factor systems are linked to enterococcal diseases that affect the health of humans and other host models. Therefore, there is substantial interest in understanding and targeting these regulatory pathways to develop novel therapies for enterococcal infection control. Quorum-sensing inhibitors could be potential therapeutic agents for attenuating the pathogenic effects of E. faecalis. Here, we discuss the regulation of cytolysin, the LuxS system, and the Fsr system, their role in E. faecalis-mediated infections, and possible therapeutic approaches to prevent E. faecalis infection.

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Mechanisms of antibiotic resistance to enrofloxacin in uropathogenic Escherichia coli in dog

Cited by 36 publications

References 76 publications

Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E. coli LPS challenge

Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E. coli LPS challenge

Antibiotic Resistance Determinant-Focused Acinetobacter baumannii Vaccine Designed Using Reverse Vaccinology

Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches

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